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Embryonic Hyperglycemia Delays the Development of Retinal Synapses in a Zebrafish Model
Embryonic hyperglycemia negatively impacts retinal development, leading to abnormal visual behavior, altered timing of retinal progenitor differentiation, decreased numbers of retinal ganglion cells and Müller glia, and vascular leakage. Because synaptic disorganization is a prominent feature of man...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456524/ https://www.ncbi.nlm.nih.gov/pubmed/36077087 http://dx.doi.org/10.3390/ijms23179693 |
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author | Shrestha, Abhishek P. Saravanakumar, Ambalavanan Konadu, Bridget Madireddy, Saivikram Gibert, Yann Vaithianathan, Thirumalini |
author_facet | Shrestha, Abhishek P. Saravanakumar, Ambalavanan Konadu, Bridget Madireddy, Saivikram Gibert, Yann Vaithianathan, Thirumalini |
author_sort | Shrestha, Abhishek P. |
collection | PubMed |
description | Embryonic hyperglycemia negatively impacts retinal development, leading to abnormal visual behavior, altered timing of retinal progenitor differentiation, decreased numbers of retinal ganglion cells and Müller glia, and vascular leakage. Because synaptic disorganization is a prominent feature of many neurological diseases, the goal of the current work was to study the potential impact of hyperglycemia on retinal ribbon synapses during embryonic development. Our approach utilized reverse transcription quantitative PCR (RT-qPCR) and immunofluorescence labeling to compare the transcription of synaptic proteins and their localization in hyperglycemic zebrafish embryos, respectively. Our data revealed that the maturity of synaptic ribbons was compromised in hyperglycemic zebrafish larvae, where altered ribeye expression coincided with the delay in establishing retinal ribbon synapses and an increase in the immature synaptic ribbons. Our results suggested that embryonic hyperglycemia disrupts retinal synapses by altering the development of the synaptic ribbon, which can lead to visual defects. Future studies using zebrafish models of hyperglycemia will allow us to study the underlying mechanisms of retinal synapse development. |
format | Online Article Text |
id | pubmed-9456524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94565242022-09-09 Embryonic Hyperglycemia Delays the Development of Retinal Synapses in a Zebrafish Model Shrestha, Abhishek P. Saravanakumar, Ambalavanan Konadu, Bridget Madireddy, Saivikram Gibert, Yann Vaithianathan, Thirumalini Int J Mol Sci Article Embryonic hyperglycemia negatively impacts retinal development, leading to abnormal visual behavior, altered timing of retinal progenitor differentiation, decreased numbers of retinal ganglion cells and Müller glia, and vascular leakage. Because synaptic disorganization is a prominent feature of many neurological diseases, the goal of the current work was to study the potential impact of hyperglycemia on retinal ribbon synapses during embryonic development. Our approach utilized reverse transcription quantitative PCR (RT-qPCR) and immunofluorescence labeling to compare the transcription of synaptic proteins and their localization in hyperglycemic zebrafish embryos, respectively. Our data revealed that the maturity of synaptic ribbons was compromised in hyperglycemic zebrafish larvae, where altered ribeye expression coincided with the delay in establishing retinal ribbon synapses and an increase in the immature synaptic ribbons. Our results suggested that embryonic hyperglycemia disrupts retinal synapses by altering the development of the synaptic ribbon, which can lead to visual defects. Future studies using zebrafish models of hyperglycemia will allow us to study the underlying mechanisms of retinal synapse development. MDPI 2022-08-26 /pmc/articles/PMC9456524/ /pubmed/36077087 http://dx.doi.org/10.3390/ijms23179693 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shrestha, Abhishek P. Saravanakumar, Ambalavanan Konadu, Bridget Madireddy, Saivikram Gibert, Yann Vaithianathan, Thirumalini Embryonic Hyperglycemia Delays the Development of Retinal Synapses in a Zebrafish Model |
title | Embryonic Hyperglycemia Delays the Development of Retinal Synapses in a Zebrafish Model |
title_full | Embryonic Hyperglycemia Delays the Development of Retinal Synapses in a Zebrafish Model |
title_fullStr | Embryonic Hyperglycemia Delays the Development of Retinal Synapses in a Zebrafish Model |
title_full_unstemmed | Embryonic Hyperglycemia Delays the Development of Retinal Synapses in a Zebrafish Model |
title_short | Embryonic Hyperglycemia Delays the Development of Retinal Synapses in a Zebrafish Model |
title_sort | embryonic hyperglycemia delays the development of retinal synapses in a zebrafish model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456524/ https://www.ncbi.nlm.nih.gov/pubmed/36077087 http://dx.doi.org/10.3390/ijms23179693 |
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