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Embryonic Hyperglycemia Delays the Development of Retinal Synapses in a Zebrafish Model

Embryonic hyperglycemia negatively impacts retinal development, leading to abnormal visual behavior, altered timing of retinal progenitor differentiation, decreased numbers of retinal ganglion cells and Müller glia, and vascular leakage. Because synaptic disorganization is a prominent feature of man...

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Autores principales: Shrestha, Abhishek P., Saravanakumar, Ambalavanan, Konadu, Bridget, Madireddy, Saivikram, Gibert, Yann, Vaithianathan, Thirumalini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456524/
https://www.ncbi.nlm.nih.gov/pubmed/36077087
http://dx.doi.org/10.3390/ijms23179693
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author Shrestha, Abhishek P.
Saravanakumar, Ambalavanan
Konadu, Bridget
Madireddy, Saivikram
Gibert, Yann
Vaithianathan, Thirumalini
author_facet Shrestha, Abhishek P.
Saravanakumar, Ambalavanan
Konadu, Bridget
Madireddy, Saivikram
Gibert, Yann
Vaithianathan, Thirumalini
author_sort Shrestha, Abhishek P.
collection PubMed
description Embryonic hyperglycemia negatively impacts retinal development, leading to abnormal visual behavior, altered timing of retinal progenitor differentiation, decreased numbers of retinal ganglion cells and Müller glia, and vascular leakage. Because synaptic disorganization is a prominent feature of many neurological diseases, the goal of the current work was to study the potential impact of hyperglycemia on retinal ribbon synapses during embryonic development. Our approach utilized reverse transcription quantitative PCR (RT-qPCR) and immunofluorescence labeling to compare the transcription of synaptic proteins and their localization in hyperglycemic zebrafish embryos, respectively. Our data revealed that the maturity of synaptic ribbons was compromised in hyperglycemic zebrafish larvae, where altered ribeye expression coincided with the delay in establishing retinal ribbon synapses and an increase in the immature synaptic ribbons. Our results suggested that embryonic hyperglycemia disrupts retinal synapses by altering the development of the synaptic ribbon, which can lead to visual defects. Future studies using zebrafish models of hyperglycemia will allow us to study the underlying mechanisms of retinal synapse development.
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spelling pubmed-94565242022-09-09 Embryonic Hyperglycemia Delays the Development of Retinal Synapses in a Zebrafish Model Shrestha, Abhishek P. Saravanakumar, Ambalavanan Konadu, Bridget Madireddy, Saivikram Gibert, Yann Vaithianathan, Thirumalini Int J Mol Sci Article Embryonic hyperglycemia negatively impacts retinal development, leading to abnormal visual behavior, altered timing of retinal progenitor differentiation, decreased numbers of retinal ganglion cells and Müller glia, and vascular leakage. Because synaptic disorganization is a prominent feature of many neurological diseases, the goal of the current work was to study the potential impact of hyperglycemia on retinal ribbon synapses during embryonic development. Our approach utilized reverse transcription quantitative PCR (RT-qPCR) and immunofluorescence labeling to compare the transcription of synaptic proteins and their localization in hyperglycemic zebrafish embryos, respectively. Our data revealed that the maturity of synaptic ribbons was compromised in hyperglycemic zebrafish larvae, where altered ribeye expression coincided with the delay in establishing retinal ribbon synapses and an increase in the immature synaptic ribbons. Our results suggested that embryonic hyperglycemia disrupts retinal synapses by altering the development of the synaptic ribbon, which can lead to visual defects. Future studies using zebrafish models of hyperglycemia will allow us to study the underlying mechanisms of retinal synapse development. MDPI 2022-08-26 /pmc/articles/PMC9456524/ /pubmed/36077087 http://dx.doi.org/10.3390/ijms23179693 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shrestha, Abhishek P.
Saravanakumar, Ambalavanan
Konadu, Bridget
Madireddy, Saivikram
Gibert, Yann
Vaithianathan, Thirumalini
Embryonic Hyperglycemia Delays the Development of Retinal Synapses in a Zebrafish Model
title Embryonic Hyperglycemia Delays the Development of Retinal Synapses in a Zebrafish Model
title_full Embryonic Hyperglycemia Delays the Development of Retinal Synapses in a Zebrafish Model
title_fullStr Embryonic Hyperglycemia Delays the Development of Retinal Synapses in a Zebrafish Model
title_full_unstemmed Embryonic Hyperglycemia Delays the Development of Retinal Synapses in a Zebrafish Model
title_short Embryonic Hyperglycemia Delays the Development of Retinal Synapses in a Zebrafish Model
title_sort embryonic hyperglycemia delays the development of retinal synapses in a zebrafish model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456524/
https://www.ncbi.nlm.nih.gov/pubmed/36077087
http://dx.doi.org/10.3390/ijms23179693
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