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Supramolecular Binding with Lectins: A New Route for Non-Covalent Functionalization of Polysaccharide Matrices

The chemical functionalization of polysaccharides to obtain functional materials has been of great interest in the last decades. This traditional synthetic approach has drawbacks, such as changing the crystallinity of the material or altering its morphology or texture. These modifications are crucia...

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Autores principales: Montroni, Devis, Di Giosia, Matteo, Calvaresi, Matteo, Falini, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9457544/
https://www.ncbi.nlm.nih.gov/pubmed/36080399
http://dx.doi.org/10.3390/molecules27175633
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author Montroni, Devis
Di Giosia, Matteo
Calvaresi, Matteo
Falini, Giuseppe
author_facet Montroni, Devis
Di Giosia, Matteo
Calvaresi, Matteo
Falini, Giuseppe
author_sort Montroni, Devis
collection PubMed
description The chemical functionalization of polysaccharides to obtain functional materials has been of great interest in the last decades. This traditional synthetic approach has drawbacks, such as changing the crystallinity of the material or altering its morphology or texture. These modifications are crucial when a biogenic matrix is exploited for its hierarchical structure. In this work, the use of lectins and carbohydrate-binding proteins as supramolecular linkers for polysaccharide functionalization is proposed. As proof of concept, a deproteinized squid pen, a hierarchically-organized β-chitin matrix, was functionalized using a dye (FITC) labeled lectin; the lectin used was the wheat germ agglutinin (WGA). It has been observed that the binding of this functionalized protein homogenously introduces a new property (fluorescence) into the β-chitin matrix without altering its crystallographic and hierarchical structure. The supramolecular functionalization of polysaccharides with protein/lectin molecules opens up new routes for the chemical modification of polysaccharides. This novel approach can be of interest in various scientific fields, overcoming the synthetic limits that have hitherto hindered the technological exploitation of polysaccharides-based materials.
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spelling pubmed-94575442022-09-09 Supramolecular Binding with Lectins: A New Route for Non-Covalent Functionalization of Polysaccharide Matrices Montroni, Devis Di Giosia, Matteo Calvaresi, Matteo Falini, Giuseppe Molecules Communication The chemical functionalization of polysaccharides to obtain functional materials has been of great interest in the last decades. This traditional synthetic approach has drawbacks, such as changing the crystallinity of the material or altering its morphology or texture. These modifications are crucial when a biogenic matrix is exploited for its hierarchical structure. In this work, the use of lectins and carbohydrate-binding proteins as supramolecular linkers for polysaccharide functionalization is proposed. As proof of concept, a deproteinized squid pen, a hierarchically-organized β-chitin matrix, was functionalized using a dye (FITC) labeled lectin; the lectin used was the wheat germ agglutinin (WGA). It has been observed that the binding of this functionalized protein homogenously introduces a new property (fluorescence) into the β-chitin matrix without altering its crystallographic and hierarchical structure. The supramolecular functionalization of polysaccharides with protein/lectin molecules opens up new routes for the chemical modification of polysaccharides. This novel approach can be of interest in various scientific fields, overcoming the synthetic limits that have hitherto hindered the technological exploitation of polysaccharides-based materials. MDPI 2022-09-01 /pmc/articles/PMC9457544/ /pubmed/36080399 http://dx.doi.org/10.3390/molecules27175633 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Montroni, Devis
Di Giosia, Matteo
Calvaresi, Matteo
Falini, Giuseppe
Supramolecular Binding with Lectins: A New Route for Non-Covalent Functionalization of Polysaccharide Matrices
title Supramolecular Binding with Lectins: A New Route for Non-Covalent Functionalization of Polysaccharide Matrices
title_full Supramolecular Binding with Lectins: A New Route for Non-Covalent Functionalization of Polysaccharide Matrices
title_fullStr Supramolecular Binding with Lectins: A New Route for Non-Covalent Functionalization of Polysaccharide Matrices
title_full_unstemmed Supramolecular Binding with Lectins: A New Route for Non-Covalent Functionalization of Polysaccharide Matrices
title_short Supramolecular Binding with Lectins: A New Route for Non-Covalent Functionalization of Polysaccharide Matrices
title_sort supramolecular binding with lectins: a new route for non-covalent functionalization of polysaccharide matrices
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9457544/
https://www.ncbi.nlm.nih.gov/pubmed/36080399
http://dx.doi.org/10.3390/molecules27175633
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