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Supramolecular Binding with Lectins: A New Route for Non-Covalent Functionalization of Polysaccharide Matrices
The chemical functionalization of polysaccharides to obtain functional materials has been of great interest in the last decades. This traditional synthetic approach has drawbacks, such as changing the crystallinity of the material or altering its morphology or texture. These modifications are crucia...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9457544/ https://www.ncbi.nlm.nih.gov/pubmed/36080399 http://dx.doi.org/10.3390/molecules27175633 |
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author | Montroni, Devis Di Giosia, Matteo Calvaresi, Matteo Falini, Giuseppe |
author_facet | Montroni, Devis Di Giosia, Matteo Calvaresi, Matteo Falini, Giuseppe |
author_sort | Montroni, Devis |
collection | PubMed |
description | The chemical functionalization of polysaccharides to obtain functional materials has been of great interest in the last decades. This traditional synthetic approach has drawbacks, such as changing the crystallinity of the material or altering its morphology or texture. These modifications are crucial when a biogenic matrix is exploited for its hierarchical structure. In this work, the use of lectins and carbohydrate-binding proteins as supramolecular linkers for polysaccharide functionalization is proposed. As proof of concept, a deproteinized squid pen, a hierarchically-organized β-chitin matrix, was functionalized using a dye (FITC) labeled lectin; the lectin used was the wheat germ agglutinin (WGA). It has been observed that the binding of this functionalized protein homogenously introduces a new property (fluorescence) into the β-chitin matrix without altering its crystallographic and hierarchical structure. The supramolecular functionalization of polysaccharides with protein/lectin molecules opens up new routes for the chemical modification of polysaccharides. This novel approach can be of interest in various scientific fields, overcoming the synthetic limits that have hitherto hindered the technological exploitation of polysaccharides-based materials. |
format | Online Article Text |
id | pubmed-9457544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94575442022-09-09 Supramolecular Binding with Lectins: A New Route for Non-Covalent Functionalization of Polysaccharide Matrices Montroni, Devis Di Giosia, Matteo Calvaresi, Matteo Falini, Giuseppe Molecules Communication The chemical functionalization of polysaccharides to obtain functional materials has been of great interest in the last decades. This traditional synthetic approach has drawbacks, such as changing the crystallinity of the material or altering its morphology or texture. These modifications are crucial when a biogenic matrix is exploited for its hierarchical structure. In this work, the use of lectins and carbohydrate-binding proteins as supramolecular linkers for polysaccharide functionalization is proposed. As proof of concept, a deproteinized squid pen, a hierarchically-organized β-chitin matrix, was functionalized using a dye (FITC) labeled lectin; the lectin used was the wheat germ agglutinin (WGA). It has been observed that the binding of this functionalized protein homogenously introduces a new property (fluorescence) into the β-chitin matrix without altering its crystallographic and hierarchical structure. The supramolecular functionalization of polysaccharides with protein/lectin molecules opens up new routes for the chemical modification of polysaccharides. This novel approach can be of interest in various scientific fields, overcoming the synthetic limits that have hitherto hindered the technological exploitation of polysaccharides-based materials. MDPI 2022-09-01 /pmc/articles/PMC9457544/ /pubmed/36080399 http://dx.doi.org/10.3390/molecules27175633 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Montroni, Devis Di Giosia, Matteo Calvaresi, Matteo Falini, Giuseppe Supramolecular Binding with Lectins: A New Route for Non-Covalent Functionalization of Polysaccharide Matrices |
title | Supramolecular Binding with Lectins: A New Route for Non-Covalent Functionalization of Polysaccharide Matrices |
title_full | Supramolecular Binding with Lectins: A New Route for Non-Covalent Functionalization of Polysaccharide Matrices |
title_fullStr | Supramolecular Binding with Lectins: A New Route for Non-Covalent Functionalization of Polysaccharide Matrices |
title_full_unstemmed | Supramolecular Binding with Lectins: A New Route for Non-Covalent Functionalization of Polysaccharide Matrices |
title_short | Supramolecular Binding with Lectins: A New Route for Non-Covalent Functionalization of Polysaccharide Matrices |
title_sort | supramolecular binding with lectins: a new route for non-covalent functionalization of polysaccharide matrices |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9457544/ https://www.ncbi.nlm.nih.gov/pubmed/36080399 http://dx.doi.org/10.3390/molecules27175633 |
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