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Biologically Active Preparations from the Leaves of Wild Plant Species of the Genus Rubus

The plants of the genus Rubus (R.) are applied as antiseptic agents in the treatment of skin diseases. Despite the great interest in plants of this genus, there are few reports on the antioxidant and biological activities of preparations obtained from the leaves of these plants. Therefore, we decide...

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Autores principales: Kucharski, Łukasz, Cybulska, Krystyna, Kucharska, Edyta, Nowak, Anna, Pełech, Robert, Klimowicz, Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9457741/
https://www.ncbi.nlm.nih.gov/pubmed/36080251
http://dx.doi.org/10.3390/molecules27175486
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author Kucharski, Łukasz
Cybulska, Krystyna
Kucharska, Edyta
Nowak, Anna
Pełech, Robert
Klimowicz, Adam
author_facet Kucharski, Łukasz
Cybulska, Krystyna
Kucharska, Edyta
Nowak, Anna
Pełech, Robert
Klimowicz, Adam
author_sort Kucharski, Łukasz
collection PubMed
description The plants of the genus Rubus (R.) are applied as antiseptic agents in the treatment of skin diseases. Despite the great interest in plants of this genus, there are few reports on the antioxidant and biological activities of preparations obtained from the leaves of these plants. Therefore, we decided to evaluate the antioxidant activity of preparations from leaves of wild plant species of the genus Rubus using the frequently applied DPPH, ABTS, and FRAP methods, as well as to determine the total polyphenol content using the Folin–Ciocalteau method and perform qualitative evaluation by gas chromatography–mass spectrometry (GC-MS). The bactericidal and fungicidal activities of the obtained preparations were evaluated by applying laboratory tests: using the disc and the well methods based on the standards EN 13697:2019, EN 13697:2015, and EN 1500:2013. Microbiological tests of the plant preparations against bacteria, fungi, and yeasts isolated from the environment and against reference strains were performed. Moreover, antimicrobial testing of antibiotics against the tested strains was performed for comparison. The n-octanol/water partition coefficient of the obtained preparations was determined by the shake-flask method to determine their lipophilicity. According to the results, a high content of polyphenols and other antioxidant and biologically active compounds can be thought of as the parameter responsible for the effective activity of plant preparations obtained from wild plant species of the genus Rubus. The methods for determining bactericidal and fungicidal activity clearly demonstrates that preparations with reduced ethanol content exhibit bactericidal and fungicidal activity on surfaces. Testing of hand disinfection by means of rubbing with the preparations confirmed their antimicrobial activity against Escherichia coli K12 NCTC 10538. The obtained results show that the tested preparations exhibit on average two times lower activity against the reference bacterial strains than comparable antibiotics. The preparations obtained from the leaves of R. idaeus L. and R. fruticosus L. could complement classical antibiotics. While environmental bacteria showed a similar response to the preparations and antibiotics, their sensitivity was about one-third less than that of the reference strains. Our studies have shown that the obtained preparations are highly hydrophilic (logP < 0). Thus, these preparations can only be used in lipid bilayers in the aqueous core of liposomes, not in the lipid envelope.
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spelling pubmed-94577412022-09-09 Biologically Active Preparations from the Leaves of Wild Plant Species of the Genus Rubus Kucharski, Łukasz Cybulska, Krystyna Kucharska, Edyta Nowak, Anna Pełech, Robert Klimowicz, Adam Molecules Article The plants of the genus Rubus (R.) are applied as antiseptic agents in the treatment of skin diseases. Despite the great interest in plants of this genus, there are few reports on the antioxidant and biological activities of preparations obtained from the leaves of these plants. Therefore, we decided to evaluate the antioxidant activity of preparations from leaves of wild plant species of the genus Rubus using the frequently applied DPPH, ABTS, and FRAP methods, as well as to determine the total polyphenol content using the Folin–Ciocalteau method and perform qualitative evaluation by gas chromatography–mass spectrometry (GC-MS). The bactericidal and fungicidal activities of the obtained preparations were evaluated by applying laboratory tests: using the disc and the well methods based on the standards EN 13697:2019, EN 13697:2015, and EN 1500:2013. Microbiological tests of the plant preparations against bacteria, fungi, and yeasts isolated from the environment and against reference strains were performed. Moreover, antimicrobial testing of antibiotics against the tested strains was performed for comparison. The n-octanol/water partition coefficient of the obtained preparations was determined by the shake-flask method to determine their lipophilicity. According to the results, a high content of polyphenols and other antioxidant and biologically active compounds can be thought of as the parameter responsible for the effective activity of plant preparations obtained from wild plant species of the genus Rubus. The methods for determining bactericidal and fungicidal activity clearly demonstrates that preparations with reduced ethanol content exhibit bactericidal and fungicidal activity on surfaces. Testing of hand disinfection by means of rubbing with the preparations confirmed their antimicrobial activity against Escherichia coli K12 NCTC 10538. The obtained results show that the tested preparations exhibit on average two times lower activity against the reference bacterial strains than comparable antibiotics. The preparations obtained from the leaves of R. idaeus L. and R. fruticosus L. could complement classical antibiotics. While environmental bacteria showed a similar response to the preparations and antibiotics, their sensitivity was about one-third less than that of the reference strains. Our studies have shown that the obtained preparations are highly hydrophilic (logP < 0). Thus, these preparations can only be used in lipid bilayers in the aqueous core of liposomes, not in the lipid envelope. MDPI 2022-08-26 /pmc/articles/PMC9457741/ /pubmed/36080251 http://dx.doi.org/10.3390/molecules27175486 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kucharski, Łukasz
Cybulska, Krystyna
Kucharska, Edyta
Nowak, Anna
Pełech, Robert
Klimowicz, Adam
Biologically Active Preparations from the Leaves of Wild Plant Species of the Genus Rubus
title Biologically Active Preparations from the Leaves of Wild Plant Species of the Genus Rubus
title_full Biologically Active Preparations from the Leaves of Wild Plant Species of the Genus Rubus
title_fullStr Biologically Active Preparations from the Leaves of Wild Plant Species of the Genus Rubus
title_full_unstemmed Biologically Active Preparations from the Leaves of Wild Plant Species of the Genus Rubus
title_short Biologically Active Preparations from the Leaves of Wild Plant Species of the Genus Rubus
title_sort biologically active preparations from the leaves of wild plant species of the genus rubus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9457741/
https://www.ncbi.nlm.nih.gov/pubmed/36080251
http://dx.doi.org/10.3390/molecules27175486
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