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Exosome Proteomics Reveals the Deregulation of Coagulation, Complement and Lipid Metabolism Proteins in Gestational Diabetes Mellitus

Exosomes are small extracellular vesicles with a variable protein cargo in consonance with cell origin and pathophysiological conditions. Gestational diabetes mellitus (GDM) is characterized by different levels of chronic low-grade inflammation and vascular dysfunction; however, there are few data c...

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Autores principales: Bernea, Elena G., Suica, Viorel I., Uyy, Elena, Cerveanu-Hogas, Aurel, Boteanu, Raluca M., Ivan, Luminita, Ceausu, Iuliana, Mihai, Doina A., Ionescu-Tîrgoviște, Constantin, Antohe, Felicia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9457917/
https://www.ncbi.nlm.nih.gov/pubmed/36080270
http://dx.doi.org/10.3390/molecules27175502
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author Bernea, Elena G.
Suica, Viorel I.
Uyy, Elena
Cerveanu-Hogas, Aurel
Boteanu, Raluca M.
Ivan, Luminita
Ceausu, Iuliana
Mihai, Doina A.
Ionescu-Tîrgoviște, Constantin
Antohe, Felicia
author_facet Bernea, Elena G.
Suica, Viorel I.
Uyy, Elena
Cerveanu-Hogas, Aurel
Boteanu, Raluca M.
Ivan, Luminita
Ceausu, Iuliana
Mihai, Doina A.
Ionescu-Tîrgoviște, Constantin
Antohe, Felicia
author_sort Bernea, Elena G.
collection PubMed
description Exosomes are small extracellular vesicles with a variable protein cargo in consonance with cell origin and pathophysiological conditions. Gestational diabetes mellitus (GDM) is characterized by different levels of chronic low-grade inflammation and vascular dysfunction; however, there are few data characterizing the serum exosomal protein cargo of GDM patients and associated signaling pathways. Eighteen pregnant women were enrolled in the study: 8 controls (CG) and 10 patients with GDM. Blood samples were collected from patients, for exosomes’ concentration. Protein abundance alterations were demonstrated by relative mass spectrometric analysis and their association with clinical parameters in GDM patients was performed using Pearson’s correlation analysis. The proteomics analysis revealed 78 significantly altered proteins when comparing GDM to CG, related to complement and coagulation cascades, platelet activation, prothrombotic factors and cholesterol metabolism. Down-regulation of Complement C3 (C3), Complement C5 (C5), C4-B (C4B), C4b-binding protein beta chain (C4BPB) and C4b-binding protein alpha chain (C4BPA), and up-regulation of C7, C9 and F12 were found in GDM. Our data indicated significant correlations between factors involved in the pathogenesis of GDM and clinical parameters that may improve the understanding of GDM pathophysiology. Data are available via ProteomeXchange with identifier PXD035673.
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spelling pubmed-94579172022-09-09 Exosome Proteomics Reveals the Deregulation of Coagulation, Complement and Lipid Metabolism Proteins in Gestational Diabetes Mellitus Bernea, Elena G. Suica, Viorel I. Uyy, Elena Cerveanu-Hogas, Aurel Boteanu, Raluca M. Ivan, Luminita Ceausu, Iuliana Mihai, Doina A. Ionescu-Tîrgoviște, Constantin Antohe, Felicia Molecules Article Exosomes are small extracellular vesicles with a variable protein cargo in consonance with cell origin and pathophysiological conditions. Gestational diabetes mellitus (GDM) is characterized by different levels of chronic low-grade inflammation and vascular dysfunction; however, there are few data characterizing the serum exosomal protein cargo of GDM patients and associated signaling pathways. Eighteen pregnant women were enrolled in the study: 8 controls (CG) and 10 patients with GDM. Blood samples were collected from patients, for exosomes’ concentration. Protein abundance alterations were demonstrated by relative mass spectrometric analysis and their association with clinical parameters in GDM patients was performed using Pearson’s correlation analysis. The proteomics analysis revealed 78 significantly altered proteins when comparing GDM to CG, related to complement and coagulation cascades, platelet activation, prothrombotic factors and cholesterol metabolism. Down-regulation of Complement C3 (C3), Complement C5 (C5), C4-B (C4B), C4b-binding protein beta chain (C4BPB) and C4b-binding protein alpha chain (C4BPA), and up-regulation of C7, C9 and F12 were found in GDM. Our data indicated significant correlations between factors involved in the pathogenesis of GDM and clinical parameters that may improve the understanding of GDM pathophysiology. Data are available via ProteomeXchange with identifier PXD035673. MDPI 2022-08-26 /pmc/articles/PMC9457917/ /pubmed/36080270 http://dx.doi.org/10.3390/molecules27175502 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bernea, Elena G.
Suica, Viorel I.
Uyy, Elena
Cerveanu-Hogas, Aurel
Boteanu, Raluca M.
Ivan, Luminita
Ceausu, Iuliana
Mihai, Doina A.
Ionescu-Tîrgoviște, Constantin
Antohe, Felicia
Exosome Proteomics Reveals the Deregulation of Coagulation, Complement and Lipid Metabolism Proteins in Gestational Diabetes Mellitus
title Exosome Proteomics Reveals the Deregulation of Coagulation, Complement and Lipid Metabolism Proteins in Gestational Diabetes Mellitus
title_full Exosome Proteomics Reveals the Deregulation of Coagulation, Complement and Lipid Metabolism Proteins in Gestational Diabetes Mellitus
title_fullStr Exosome Proteomics Reveals the Deregulation of Coagulation, Complement and Lipid Metabolism Proteins in Gestational Diabetes Mellitus
title_full_unstemmed Exosome Proteomics Reveals the Deregulation of Coagulation, Complement and Lipid Metabolism Proteins in Gestational Diabetes Mellitus
title_short Exosome Proteomics Reveals the Deregulation of Coagulation, Complement and Lipid Metabolism Proteins in Gestational Diabetes Mellitus
title_sort exosome proteomics reveals the deregulation of coagulation, complement and lipid metabolism proteins in gestational diabetes mellitus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9457917/
https://www.ncbi.nlm.nih.gov/pubmed/36080270
http://dx.doi.org/10.3390/molecules27175502
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