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Degradative Effect of Nattokinase on Spike Protein of SARS-CoV-2
The coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged as a pandemic and has inflicted enormous damage on the lives of the people and economy of many countries worldwide. However, therapeutic agents against SARS-CoV-2 remain uncle...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9458005/ https://www.ncbi.nlm.nih.gov/pubmed/36080170 http://dx.doi.org/10.3390/molecules27175405 |
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author | Tanikawa, Takashi Kiba, Yuka Yu, James Hsu, Kate Chen, Shinder Ishii, Ayako Yokogawa, Takami Suzuki, Ryuichiro Inoue, Yutaka Kitamura, Masashi |
author_facet | Tanikawa, Takashi Kiba, Yuka Yu, James Hsu, Kate Chen, Shinder Ishii, Ayako Yokogawa, Takami Suzuki, Ryuichiro Inoue, Yutaka Kitamura, Masashi |
author_sort | Tanikawa, Takashi |
collection | PubMed |
description | The coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged as a pandemic and has inflicted enormous damage on the lives of the people and economy of many countries worldwide. However, therapeutic agents against SARS-CoV-2 remain unclear. SARS-CoV-2 has a spike protein (S protein), and cleavage of the S protein is essential for viral entry. Nattokinase is produced by Bacillus subtilis var. natto and is beneficial to human health. In this study, we examined the effect of nattokinase on the S protein of SARS-CoV-2. When cell lysates transfected with S protein were incubated with nattokinase, the S protein was degraded in a dose- and time-dependent manner. Immunofluorescence analysis showed that S protein on the cell surface was degraded when nattokinase was added to the culture medium. Thus, our findings suggest that nattokinase exhibits potential for the inhibition of SARS-CoV-2 infection via S protein degradation. |
format | Online Article Text |
id | pubmed-9458005 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94580052022-09-09 Degradative Effect of Nattokinase on Spike Protein of SARS-CoV-2 Tanikawa, Takashi Kiba, Yuka Yu, James Hsu, Kate Chen, Shinder Ishii, Ayako Yokogawa, Takami Suzuki, Ryuichiro Inoue, Yutaka Kitamura, Masashi Molecules Article The coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged as a pandemic and has inflicted enormous damage on the lives of the people and economy of many countries worldwide. However, therapeutic agents against SARS-CoV-2 remain unclear. SARS-CoV-2 has a spike protein (S protein), and cleavage of the S protein is essential for viral entry. Nattokinase is produced by Bacillus subtilis var. natto and is beneficial to human health. In this study, we examined the effect of nattokinase on the S protein of SARS-CoV-2. When cell lysates transfected with S protein were incubated with nattokinase, the S protein was degraded in a dose- and time-dependent manner. Immunofluorescence analysis showed that S protein on the cell surface was degraded when nattokinase was added to the culture medium. Thus, our findings suggest that nattokinase exhibits potential for the inhibition of SARS-CoV-2 infection via S protein degradation. MDPI 2022-08-24 /pmc/articles/PMC9458005/ /pubmed/36080170 http://dx.doi.org/10.3390/molecules27175405 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tanikawa, Takashi Kiba, Yuka Yu, James Hsu, Kate Chen, Shinder Ishii, Ayako Yokogawa, Takami Suzuki, Ryuichiro Inoue, Yutaka Kitamura, Masashi Degradative Effect of Nattokinase on Spike Protein of SARS-CoV-2 |
title | Degradative Effect of Nattokinase on Spike Protein of SARS-CoV-2 |
title_full | Degradative Effect of Nattokinase on Spike Protein of SARS-CoV-2 |
title_fullStr | Degradative Effect of Nattokinase on Spike Protein of SARS-CoV-2 |
title_full_unstemmed | Degradative Effect of Nattokinase on Spike Protein of SARS-CoV-2 |
title_short | Degradative Effect of Nattokinase on Spike Protein of SARS-CoV-2 |
title_sort | degradative effect of nattokinase on spike protein of sars-cov-2 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9458005/ https://www.ncbi.nlm.nih.gov/pubmed/36080170 http://dx.doi.org/10.3390/molecules27175405 |
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