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Degradative Effect of Nattokinase on Spike Protein of SARS-CoV-2

The coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged as a pandemic and has inflicted enormous damage on the lives of the people and economy of many countries worldwide. However, therapeutic agents against SARS-CoV-2 remain uncle...

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Autores principales: Tanikawa, Takashi, Kiba, Yuka, Yu, James, Hsu, Kate, Chen, Shinder, Ishii, Ayako, Yokogawa, Takami, Suzuki, Ryuichiro, Inoue, Yutaka, Kitamura, Masashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9458005/
https://www.ncbi.nlm.nih.gov/pubmed/36080170
http://dx.doi.org/10.3390/molecules27175405
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author Tanikawa, Takashi
Kiba, Yuka
Yu, James
Hsu, Kate
Chen, Shinder
Ishii, Ayako
Yokogawa, Takami
Suzuki, Ryuichiro
Inoue, Yutaka
Kitamura, Masashi
author_facet Tanikawa, Takashi
Kiba, Yuka
Yu, James
Hsu, Kate
Chen, Shinder
Ishii, Ayako
Yokogawa, Takami
Suzuki, Ryuichiro
Inoue, Yutaka
Kitamura, Masashi
author_sort Tanikawa, Takashi
collection PubMed
description The coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged as a pandemic and has inflicted enormous damage on the lives of the people and economy of many countries worldwide. However, therapeutic agents against SARS-CoV-2 remain unclear. SARS-CoV-2 has a spike protein (S protein), and cleavage of the S protein is essential for viral entry. Nattokinase is produced by Bacillus subtilis var. natto and is beneficial to human health. In this study, we examined the effect of nattokinase on the S protein of SARS-CoV-2. When cell lysates transfected with S protein were incubated with nattokinase, the S protein was degraded in a dose- and time-dependent manner. Immunofluorescence analysis showed that S protein on the cell surface was degraded when nattokinase was added to the culture medium. Thus, our findings suggest that nattokinase exhibits potential for the inhibition of SARS-CoV-2 infection via S protein degradation.
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spelling pubmed-94580052022-09-09 Degradative Effect of Nattokinase on Spike Protein of SARS-CoV-2 Tanikawa, Takashi Kiba, Yuka Yu, James Hsu, Kate Chen, Shinder Ishii, Ayako Yokogawa, Takami Suzuki, Ryuichiro Inoue, Yutaka Kitamura, Masashi Molecules Article The coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged as a pandemic and has inflicted enormous damage on the lives of the people and economy of many countries worldwide. However, therapeutic agents against SARS-CoV-2 remain unclear. SARS-CoV-2 has a spike protein (S protein), and cleavage of the S protein is essential for viral entry. Nattokinase is produced by Bacillus subtilis var. natto and is beneficial to human health. In this study, we examined the effect of nattokinase on the S protein of SARS-CoV-2. When cell lysates transfected with S protein were incubated with nattokinase, the S protein was degraded in a dose- and time-dependent manner. Immunofluorescence analysis showed that S protein on the cell surface was degraded when nattokinase was added to the culture medium. Thus, our findings suggest that nattokinase exhibits potential for the inhibition of SARS-CoV-2 infection via S protein degradation. MDPI 2022-08-24 /pmc/articles/PMC9458005/ /pubmed/36080170 http://dx.doi.org/10.3390/molecules27175405 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tanikawa, Takashi
Kiba, Yuka
Yu, James
Hsu, Kate
Chen, Shinder
Ishii, Ayako
Yokogawa, Takami
Suzuki, Ryuichiro
Inoue, Yutaka
Kitamura, Masashi
Degradative Effect of Nattokinase on Spike Protein of SARS-CoV-2
title Degradative Effect of Nattokinase on Spike Protein of SARS-CoV-2
title_full Degradative Effect of Nattokinase on Spike Protein of SARS-CoV-2
title_fullStr Degradative Effect of Nattokinase on Spike Protein of SARS-CoV-2
title_full_unstemmed Degradative Effect of Nattokinase on Spike Protein of SARS-CoV-2
title_short Degradative Effect of Nattokinase on Spike Protein of SARS-CoV-2
title_sort degradative effect of nattokinase on spike protein of sars-cov-2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9458005/
https://www.ncbi.nlm.nih.gov/pubmed/36080170
http://dx.doi.org/10.3390/molecules27175405
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