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Intensification of resveratrol cytotoxicity, pro-apoptosis, oxidant potentials in human colorectal carcinoma HCT-116 cells using zein nanoparticles

Resveratrol (RSV), a non-flavonoid stilbene polyphenol, possesses anti-carcinogenic activities against all the major stages of cancer. Zein nanoparticles (ZN NPs) have been utilized successfully in delivery of variant therapeuticals by virtue of their histocompatible nature. The goal of this work wa...

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Autores principales: Khayat, Maan T., Zarka, Mohamed A., El-Telbany, Dalia Farag. A., El-Halawany, Ali M., Kutbi, Hussam Ibrahim, Elkhatib, Walid F., Noreddin, Ayman M., Khayyat, Ahdab N., El-Telbany, Rania Farag A., Hammad, Sherif F., Abdel-Naim, Ashraf B., Alolayan, Ebtesam M., Al-Sawahli, Majid Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9458658/
https://www.ncbi.nlm.nih.gov/pubmed/36075939
http://dx.doi.org/10.1038/s41598-022-18557-2
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author Khayat, Maan T.
Zarka, Mohamed A.
El-Telbany, Dalia Farag. A.
El-Halawany, Ali M.
Kutbi, Hussam Ibrahim
Elkhatib, Walid F.
Noreddin, Ayman M.
Khayyat, Ahdab N.
El-Telbany, Rania Farag A.
Hammad, Sherif F.
Abdel-Naim, Ashraf B.
Alolayan, Ebtesam M.
Al-Sawahli, Majid Mohammad
author_facet Khayat, Maan T.
Zarka, Mohamed A.
El-Telbany, Dalia Farag. A.
El-Halawany, Ali M.
Kutbi, Hussam Ibrahim
Elkhatib, Walid F.
Noreddin, Ayman M.
Khayyat, Ahdab N.
El-Telbany, Rania Farag A.
Hammad, Sherif F.
Abdel-Naim, Ashraf B.
Alolayan, Ebtesam M.
Al-Sawahli, Majid Mohammad
author_sort Khayat, Maan T.
collection PubMed
description Resveratrol (RSV), a non-flavonoid stilbene polyphenol, possesses anti-carcinogenic activities against all the major stages of cancer. Zein nanoparticles (ZN NPs) have been utilized successfully in delivery of variant therapeuticals by virtue of their histocompatible nature. The goal of this work was to comparatively explore the antiproliferative, pro-apoptotic and oxidative stress potentials of RSV-ZN NPs versus RSV against human colorectal carcinoma HCT-116 cells. ZN-RSV NPs were developed and assayed for particle size analysis and RSV diffusion. The selected formula obtained 137.6 ± 8.3 nm as mean particle size, 29.4 ± 1.8 mV zeta potential, 92.3 ± 3.6% encapsulation efficiency. IC(50) of the selected formula was significantly lower against HCT-116 cells versus Caco-2 cells. Also, significantly enhanced cellular uptake was generated from RSV-ZN NPs versus free RSV. Enhanced apoptosis was concluded due to increased percentage cells in G2-M and pre-G1 phases. The pro-apoptotic potential was explained by caspase-3 and cleaved caspase-3 increased mRNA expression in addition to NF-κB and miRNA125b decreased expression. Biochemically, ZN-RSV NPs induced oxidative stress as demonstrated by enhanced reactive oxygen species (ROS) generation and endothelial nitric oxide synthase (eNOS) isoenzyme increased levels. Conclusively, ZN-RSV NPs obtained cell cycle inhibition supported with augmented cytotoxicity, uptake and oxidative stress markers levels in HCT-116 tumor cells in comparison with free RSV. These results indicated intensified chemopreventive profile of RSV due to effective delivery utilizing ZN nano-dispersion against colorectal carcinoma HCT-116 cells.
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spelling pubmed-94586582022-09-10 Intensification of resveratrol cytotoxicity, pro-apoptosis, oxidant potentials in human colorectal carcinoma HCT-116 cells using zein nanoparticles Khayat, Maan T. Zarka, Mohamed A. El-Telbany, Dalia Farag. A. El-Halawany, Ali M. Kutbi, Hussam Ibrahim Elkhatib, Walid F. Noreddin, Ayman M. Khayyat, Ahdab N. El-Telbany, Rania Farag A. Hammad, Sherif F. Abdel-Naim, Ashraf B. Alolayan, Ebtesam M. Al-Sawahli, Majid Mohammad Sci Rep Article Resveratrol (RSV), a non-flavonoid stilbene polyphenol, possesses anti-carcinogenic activities against all the major stages of cancer. Zein nanoparticles (ZN NPs) have been utilized successfully in delivery of variant therapeuticals by virtue of their histocompatible nature. The goal of this work was to comparatively explore the antiproliferative, pro-apoptotic and oxidative stress potentials of RSV-ZN NPs versus RSV against human colorectal carcinoma HCT-116 cells. ZN-RSV NPs were developed and assayed for particle size analysis and RSV diffusion. The selected formula obtained 137.6 ± 8.3 nm as mean particle size, 29.4 ± 1.8 mV zeta potential, 92.3 ± 3.6% encapsulation efficiency. IC(50) of the selected formula was significantly lower against HCT-116 cells versus Caco-2 cells. Also, significantly enhanced cellular uptake was generated from RSV-ZN NPs versus free RSV. Enhanced apoptosis was concluded due to increased percentage cells in G2-M and pre-G1 phases. The pro-apoptotic potential was explained by caspase-3 and cleaved caspase-3 increased mRNA expression in addition to NF-κB and miRNA125b decreased expression. Biochemically, ZN-RSV NPs induced oxidative stress as demonstrated by enhanced reactive oxygen species (ROS) generation and endothelial nitric oxide synthase (eNOS) isoenzyme increased levels. Conclusively, ZN-RSV NPs obtained cell cycle inhibition supported with augmented cytotoxicity, uptake and oxidative stress markers levels in HCT-116 tumor cells in comparison with free RSV. These results indicated intensified chemopreventive profile of RSV due to effective delivery utilizing ZN nano-dispersion against colorectal carcinoma HCT-116 cells. Nature Publishing Group UK 2022-09-08 /pmc/articles/PMC9458658/ /pubmed/36075939 http://dx.doi.org/10.1038/s41598-022-18557-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Khayat, Maan T.
Zarka, Mohamed A.
El-Telbany, Dalia Farag. A.
El-Halawany, Ali M.
Kutbi, Hussam Ibrahim
Elkhatib, Walid F.
Noreddin, Ayman M.
Khayyat, Ahdab N.
El-Telbany, Rania Farag A.
Hammad, Sherif F.
Abdel-Naim, Ashraf B.
Alolayan, Ebtesam M.
Al-Sawahli, Majid Mohammad
Intensification of resveratrol cytotoxicity, pro-apoptosis, oxidant potentials in human colorectal carcinoma HCT-116 cells using zein nanoparticles
title Intensification of resveratrol cytotoxicity, pro-apoptosis, oxidant potentials in human colorectal carcinoma HCT-116 cells using zein nanoparticles
title_full Intensification of resveratrol cytotoxicity, pro-apoptosis, oxidant potentials in human colorectal carcinoma HCT-116 cells using zein nanoparticles
title_fullStr Intensification of resveratrol cytotoxicity, pro-apoptosis, oxidant potentials in human colorectal carcinoma HCT-116 cells using zein nanoparticles
title_full_unstemmed Intensification of resveratrol cytotoxicity, pro-apoptosis, oxidant potentials in human colorectal carcinoma HCT-116 cells using zein nanoparticles
title_short Intensification of resveratrol cytotoxicity, pro-apoptosis, oxidant potentials in human colorectal carcinoma HCT-116 cells using zein nanoparticles
title_sort intensification of resveratrol cytotoxicity, pro-apoptosis, oxidant potentials in human colorectal carcinoma hct-116 cells using zein nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9458658/
https://www.ncbi.nlm.nih.gov/pubmed/36075939
http://dx.doi.org/10.1038/s41598-022-18557-2
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