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Effect of temperature change on the performance of the hybrid linear flow channel reactor and its implications on sulphate-reducing and sulphide-oxidising microbial community dynamics

Semi-passive bioremediation is a promising strategy to mitigate persistent low volume mine-impacted wastewater containing high sulphate concentrations. Building on the proof of concept demonstration of the hybrid linear flow channel reactor (LFCR), capable of simultaneous biological sulphate reducti...

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Autores principales: Marais, T. S., Huddy, R. J., Van Hille, R. P., Harrison, S. T. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9458953/
https://www.ncbi.nlm.nih.gov/pubmed/36091461
http://dx.doi.org/10.3389/fbioe.2022.908463
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author Marais, T. S.
Huddy, R. J.
Van Hille, R. P.
Harrison, S. T. L.
author_facet Marais, T. S.
Huddy, R. J.
Van Hille, R. P.
Harrison, S. T. L.
author_sort Marais, T. S.
collection PubMed
description Semi-passive bioremediation is a promising strategy to mitigate persistent low volume mine-impacted wastewater containing high sulphate concentrations. Building on the proof of concept demonstration of the hybrid linear flow channel reactor (LFCR), capable of simultaneous biological sulphate reduction and partial sulphide oxidation with elemental sulphur recovery, the impact of key operating parameters, such as temperature, on process performance is critical to real-world application. Temperature fluctuates seasonally and across the diurnal cycle, impacting biological sulphate reduction (BSR) and partial sulphide oxidation. The process is reliant on the metabolic activity and synergistic interactions between sulphate-reducing (SRB) and sulphide-oxidising (SOB) microbial communities that develop within discrete oxic and anoxic microenvironments within the hybrid LFCR. In this study, the impact of operating temperature on process performance was evaluated by decreasing temperature with time from 30 to 10°C in each of three laboratory-scaled hybrid LFCR units operating in pseudo-steady state at 1 g/L sulphate. Using lactate as a carbon source, two reactor sizes (2 and 8 L) were considered, while the impact of lactate vs. acetate as carbon source was evaluated in the 2 L reactors. On incremental decrease in temperature from 30 to 10°C, a decrease in volumetric sulphate reduction rate was observed: from 0.144 to 0.059 mmol/L.h in the 2 L lactate-fed reactor; from 0.128 to 0.042 mmol/L.h in the 8 L lactate-fed reactor; and from 0.127 to 0.010 mmol/L.h in the 2 L acetate-fed reactor. Similarly, sulphate conversion efficiency decreased (2 L lactate-fed: 66% to 27%; 8 L lactate-fed: 61% to 20%; 2 L acetate-fed: 61% to 5%). A decrease in temperature below the critical value (15°C) led to considerable loss in metabolic activity and overall BSR performance. Sessile and planktonic microbial communities were represented by bacterial phyla including Proteobacteria, Synergistetes, Bacteroidetes, and Firmicutes. A diverse group of putative SRB (Deltaproteobacteria) and SOB, including Alpha, Beta, Gamma, and Epsilonproteobacteria phylotypes, were prevalent and shifted in relative abundance and community composition in response to decreasing temperature. Specifically, the decrease in the relative abundance of Deltaproteobacteria with decreasing temperature below 15°C corresponded with a loss of BSR performance across all three reactors. This study demonstrated the impact of low temperature on the physiological selection and ecological differentiation of SRB and SOB communities within the hybrid LFCR and its implications for real-world process performance.
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spelling pubmed-94589532022-09-10 Effect of temperature change on the performance of the hybrid linear flow channel reactor and its implications on sulphate-reducing and sulphide-oxidising microbial community dynamics Marais, T. S. Huddy, R. J. Van Hille, R. P. Harrison, S. T. L. Front Bioeng Biotechnol Bioengineering and Biotechnology Semi-passive bioremediation is a promising strategy to mitigate persistent low volume mine-impacted wastewater containing high sulphate concentrations. Building on the proof of concept demonstration of the hybrid linear flow channel reactor (LFCR), capable of simultaneous biological sulphate reduction and partial sulphide oxidation with elemental sulphur recovery, the impact of key operating parameters, such as temperature, on process performance is critical to real-world application. Temperature fluctuates seasonally and across the diurnal cycle, impacting biological sulphate reduction (BSR) and partial sulphide oxidation. The process is reliant on the metabolic activity and synergistic interactions between sulphate-reducing (SRB) and sulphide-oxidising (SOB) microbial communities that develop within discrete oxic and anoxic microenvironments within the hybrid LFCR. In this study, the impact of operating temperature on process performance was evaluated by decreasing temperature with time from 30 to 10°C in each of three laboratory-scaled hybrid LFCR units operating in pseudo-steady state at 1 g/L sulphate. Using lactate as a carbon source, two reactor sizes (2 and 8 L) were considered, while the impact of lactate vs. acetate as carbon source was evaluated in the 2 L reactors. On incremental decrease in temperature from 30 to 10°C, a decrease in volumetric sulphate reduction rate was observed: from 0.144 to 0.059 mmol/L.h in the 2 L lactate-fed reactor; from 0.128 to 0.042 mmol/L.h in the 8 L lactate-fed reactor; and from 0.127 to 0.010 mmol/L.h in the 2 L acetate-fed reactor. Similarly, sulphate conversion efficiency decreased (2 L lactate-fed: 66% to 27%; 8 L lactate-fed: 61% to 20%; 2 L acetate-fed: 61% to 5%). A decrease in temperature below the critical value (15°C) led to considerable loss in metabolic activity and overall BSR performance. Sessile and planktonic microbial communities were represented by bacterial phyla including Proteobacteria, Synergistetes, Bacteroidetes, and Firmicutes. A diverse group of putative SRB (Deltaproteobacteria) and SOB, including Alpha, Beta, Gamma, and Epsilonproteobacteria phylotypes, were prevalent and shifted in relative abundance and community composition in response to decreasing temperature. Specifically, the decrease in the relative abundance of Deltaproteobacteria with decreasing temperature below 15°C corresponded with a loss of BSR performance across all three reactors. This study demonstrated the impact of low temperature on the physiological selection and ecological differentiation of SRB and SOB communities within the hybrid LFCR and its implications for real-world process performance. Frontiers Media S.A. 2022-08-26 /pmc/articles/PMC9458953/ /pubmed/36091461 http://dx.doi.org/10.3389/fbioe.2022.908463 Text en Copyright © 2022 Marais, Huddy, Van Hille and Harrison. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Marais, T. S.
Huddy, R. J.
Van Hille, R. P.
Harrison, S. T. L.
Effect of temperature change on the performance of the hybrid linear flow channel reactor and its implications on sulphate-reducing and sulphide-oxidising microbial community dynamics
title Effect of temperature change on the performance of the hybrid linear flow channel reactor and its implications on sulphate-reducing and sulphide-oxidising microbial community dynamics
title_full Effect of temperature change on the performance of the hybrid linear flow channel reactor and its implications on sulphate-reducing and sulphide-oxidising microbial community dynamics
title_fullStr Effect of temperature change on the performance of the hybrid linear flow channel reactor and its implications on sulphate-reducing and sulphide-oxidising microbial community dynamics
title_full_unstemmed Effect of temperature change on the performance of the hybrid linear flow channel reactor and its implications on sulphate-reducing and sulphide-oxidising microbial community dynamics
title_short Effect of temperature change on the performance of the hybrid linear flow channel reactor and its implications on sulphate-reducing and sulphide-oxidising microbial community dynamics
title_sort effect of temperature change on the performance of the hybrid linear flow channel reactor and its implications on sulphate-reducing and sulphide-oxidising microbial community dynamics
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9458953/
https://www.ncbi.nlm.nih.gov/pubmed/36091461
http://dx.doi.org/10.3389/fbioe.2022.908463
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