Cargando…

TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheat

NAC proteins constitute one of the largest plant-specific transcription factor (TF) families and play significant roles in plant growth and development. In the present study, three TaNAC020 homoeologous genes located on chromosomes 7A, 7B, and 7D were isolated from wheat (Triticum aestivum L.). TaNA...

Descripción completa

Detalles Bibliográficos
Autores principales: Majeed, Uzma, Hou, Jian, Hao, Chenyang, Zhang, Xueyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9458977/
https://www.ncbi.nlm.nih.gov/pubmed/36092915
http://dx.doi.org/10.3389/fgene.2022.956921
_version_ 1784786399419957248
author Majeed, Uzma
Hou, Jian
Hao, Chenyang
Zhang, Xueyong
author_facet Majeed, Uzma
Hou, Jian
Hao, Chenyang
Zhang, Xueyong
author_sort Majeed, Uzma
collection PubMed
description NAC proteins constitute one of the largest plant-specific transcription factor (TF) families and play significant roles in plant growth and development. In the present study, three TaNAC020 homoeologous genes located on chromosomes 7A, 7B, and 7D were isolated from wheat (Triticum aestivum L.). TaNAC020s were predominantly expressed in developing grains. The developed transgenic rice lines for TaNAC020-B showed higher starch density and lower amylose contents than those of the wild type (WT). Sequence polymorphism studies showed seven and eight SNPs in TaNAC020-A/B, making three and two haplotypes, respectively. No sequence polymorphism was identified in TaNAC020-D. Association analysis revealed that HAP-2 of TaNAC020-A and TaNAC020-B was the favored haplotype for higher thousand kernel weight and length. Geographic distribution and allelic frequency showed that our favored haplotype experienced strong selection in China, and likewise, diversity increased in TaNAC020s during wheat polyploidization. The results obtained in this study demonstrate that TaNAC020s positively influence starch synthesis and accumulation and are one of the key regulators of the kernel (seed) size and kernel number and have the potential for utilization in wheat breeding to improve grain yield. Molecular markers developed in this study stand instrumental in marker-assisted selection for genetic improvement and germplasm enhancement in wheat.
format Online
Article
Text
id pubmed-9458977
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-94589772022-09-10 TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheat Majeed, Uzma Hou, Jian Hao, Chenyang Zhang, Xueyong Front Genet Genetics NAC proteins constitute one of the largest plant-specific transcription factor (TF) families and play significant roles in plant growth and development. In the present study, three TaNAC020 homoeologous genes located on chromosomes 7A, 7B, and 7D were isolated from wheat (Triticum aestivum L.). TaNAC020s were predominantly expressed in developing grains. The developed transgenic rice lines for TaNAC020-B showed higher starch density and lower amylose contents than those of the wild type (WT). Sequence polymorphism studies showed seven and eight SNPs in TaNAC020-A/B, making three and two haplotypes, respectively. No sequence polymorphism was identified in TaNAC020-D. Association analysis revealed that HAP-2 of TaNAC020-A and TaNAC020-B was the favored haplotype for higher thousand kernel weight and length. Geographic distribution and allelic frequency showed that our favored haplotype experienced strong selection in China, and likewise, diversity increased in TaNAC020s during wheat polyploidization. The results obtained in this study demonstrate that TaNAC020s positively influence starch synthesis and accumulation and are one of the key regulators of the kernel (seed) size and kernel number and have the potential for utilization in wheat breeding to improve grain yield. Molecular markers developed in this study stand instrumental in marker-assisted selection for genetic improvement and germplasm enhancement in wheat. Frontiers Media S.A. 2022-08-26 /pmc/articles/PMC9458977/ /pubmed/36092915 http://dx.doi.org/10.3389/fgene.2022.956921 Text en Copyright © 2022 Majeed, Hou, Hao and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Majeed, Uzma
Hou, Jian
Hao, Chenyang
Zhang, Xueyong
TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheat
title TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheat
title_full TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheat
title_fullStr TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheat
title_full_unstemmed TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheat
title_short TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheat
title_sort tanac020 homoeologous genes are associated with higher thousand kernel weight and kernel length in chinese wheat
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9458977/
https://www.ncbi.nlm.nih.gov/pubmed/36092915
http://dx.doi.org/10.3389/fgene.2022.956921
work_keys_str_mv AT majeeduzma tanac020homoeologousgenesareassociatedwithhigherthousandkernelweightandkernellengthinchinesewheat
AT houjian tanac020homoeologousgenesareassociatedwithhigherthousandkernelweightandkernellengthinchinesewheat
AT haochenyang tanac020homoeologousgenesareassociatedwithhigherthousandkernelweightandkernellengthinchinesewheat
AT zhangxueyong tanac020homoeologousgenesareassociatedwithhigherthousandkernelweightandkernellengthinchinesewheat