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Interleukin 13 participates in terminal differentiation of esophageal squamous cell carcinoma cells

BACKGROUND: In China, esophageal squamous cell carcinoma (ESCC) accounts for more than 90% of all esophageal cancer cases. Interleukin 13 (IL-13) was widely reported to play a key role in tumor progression. Our previous study reported that IL-13 was a favorable predictive marker for the overall surv...

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Autores principales: Li, Jian, Wang, Wenjian, Wang, Ke, Ma, Guangwei, Shao, Jing, Fang, Weizhen, Zhou, Yiting, Lin, Jiatong, Guo, Yabin, Guan, Xinyuan, Duan, Chaohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459211/
https://www.ncbi.nlm.nih.gov/pubmed/36092316
http://dx.doi.org/10.21037/jgo-22-559
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author Li, Jian
Wang, Wenjian
Wang, Ke
Ma, Guangwei
Shao, Jing
Fang, Weizhen
Zhou, Yiting
Lin, Jiatong
Guo, Yabin
Guan, Xinyuan
Duan, Chaohui
author_facet Li, Jian
Wang, Wenjian
Wang, Ke
Ma, Guangwei
Shao, Jing
Fang, Weizhen
Zhou, Yiting
Lin, Jiatong
Guo, Yabin
Guan, Xinyuan
Duan, Chaohui
author_sort Li, Jian
collection PubMed
description BACKGROUND: In China, esophageal squamous cell carcinoma (ESCC) accounts for more than 90% of all esophageal cancer cases. Interleukin 13 (IL-13) was widely reported to play a key role in tumor progression. Our previous study reported that IL-13 was a favorable predictive marker for the overall survival of esophageal squamous cell carcinoma (ESCC) patients, but how IL-13 contributes to ESCC progression remains unknown. This study aims to explore the role of IL-13 and its underlying downstream molecular mechanisms in ESCC progression. METHODS: Tissue microarrays including 262 primary ESCC tumor tissues were collected and analyzed. The expression of IL-13 in ESCC tumor tissue was detected with immunohistochemistry staining (IHC). Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to qualify the expressions of KRT13, KRT4 and 15-lipoxygenase-1 (15-LOX-1) in cultured ESCC cell lines with recombinant IL-13 treatment. RESULTS: IL-13 was expressed in the esophageal epithelium cells and ESCC tumor cells. High IL-13 expression in ESCC tumor cells predicted a good prognosis for patients. Recombinant human IL-13 raised KRT13 and 15-LOX-1 mRNA levels, but lowered KRT4 mRNA level 15-LOX-1 in ESCC cells in vitro. CONCLUSIONS: In summary, our study suggests that IL-13 might improve the prognosis of ESCC by promoting the terminal differentiation of ESCC cells. This may offer potential new therapeutic target for early treatment of ESCC.
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spelling pubmed-94592112022-09-10 Interleukin 13 participates in terminal differentiation of esophageal squamous cell carcinoma cells Li, Jian Wang, Wenjian Wang, Ke Ma, Guangwei Shao, Jing Fang, Weizhen Zhou, Yiting Lin, Jiatong Guo, Yabin Guan, Xinyuan Duan, Chaohui J Gastrointest Oncol Original Article BACKGROUND: In China, esophageal squamous cell carcinoma (ESCC) accounts for more than 90% of all esophageal cancer cases. Interleukin 13 (IL-13) was widely reported to play a key role in tumor progression. Our previous study reported that IL-13 was a favorable predictive marker for the overall survival of esophageal squamous cell carcinoma (ESCC) patients, but how IL-13 contributes to ESCC progression remains unknown. This study aims to explore the role of IL-13 and its underlying downstream molecular mechanisms in ESCC progression. METHODS: Tissue microarrays including 262 primary ESCC tumor tissues were collected and analyzed. The expression of IL-13 in ESCC tumor tissue was detected with immunohistochemistry staining (IHC). Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to qualify the expressions of KRT13, KRT4 and 15-lipoxygenase-1 (15-LOX-1) in cultured ESCC cell lines with recombinant IL-13 treatment. RESULTS: IL-13 was expressed in the esophageal epithelium cells and ESCC tumor cells. High IL-13 expression in ESCC tumor cells predicted a good prognosis for patients. Recombinant human IL-13 raised KRT13 and 15-LOX-1 mRNA levels, but lowered KRT4 mRNA level 15-LOX-1 in ESCC cells in vitro. CONCLUSIONS: In summary, our study suggests that IL-13 might improve the prognosis of ESCC by promoting the terminal differentiation of ESCC cells. This may offer potential new therapeutic target for early treatment of ESCC. AME Publishing Company 2022-08 /pmc/articles/PMC9459211/ /pubmed/36092316 http://dx.doi.org/10.21037/jgo-22-559 Text en 2022 Journal of Gastrointestinal Oncology. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Li, Jian
Wang, Wenjian
Wang, Ke
Ma, Guangwei
Shao, Jing
Fang, Weizhen
Zhou, Yiting
Lin, Jiatong
Guo, Yabin
Guan, Xinyuan
Duan, Chaohui
Interleukin 13 participates in terminal differentiation of esophageal squamous cell carcinoma cells
title Interleukin 13 participates in terminal differentiation of esophageal squamous cell carcinoma cells
title_full Interleukin 13 participates in terminal differentiation of esophageal squamous cell carcinoma cells
title_fullStr Interleukin 13 participates in terminal differentiation of esophageal squamous cell carcinoma cells
title_full_unstemmed Interleukin 13 participates in terminal differentiation of esophageal squamous cell carcinoma cells
title_short Interleukin 13 participates in terminal differentiation of esophageal squamous cell carcinoma cells
title_sort interleukin 13 participates in terminal differentiation of esophageal squamous cell carcinoma cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459211/
https://www.ncbi.nlm.nih.gov/pubmed/36092316
http://dx.doi.org/10.21037/jgo-22-559
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