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PagDET2 promotes cambium cell division and xylem differentiation in poplar stem

Secondary growth of the woody tree stem is governed by meristematic cell division and differentiation in the vascular cambium. Multiple hormonal signals and endogenous developmental programs regulate vascular cambium activity. Brassinosteroids (BRs) significantly promote secondary stem growth and wo...

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Autores principales: Wang, Yao, Hao, Yi, Guo, Yakun, Shou, Huixia, Du, Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459238/
https://www.ncbi.nlm.nih.gov/pubmed/36092441
http://dx.doi.org/10.3389/fpls.2022.923530
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author Wang, Yao
Hao, Yi
Guo, Yakun
Shou, Huixia
Du, Juan
author_facet Wang, Yao
Hao, Yi
Guo, Yakun
Shou, Huixia
Du, Juan
author_sort Wang, Yao
collection PubMed
description Secondary growth of the woody tree stem is governed by meristematic cell division and differentiation in the vascular cambium. Multiple hormonal signals and endogenous developmental programs regulate vascular cambium activity. Brassinosteroids (BRs) significantly promote secondary stem growth and wood formation in poplar trees. However, the underlying regulatory mechanisms of BRs within the vascular tissue remain unclear. Genetic and anatomical approaches were used here to elucidate the role of PagDET2, the rate-limiting enzyme for BRs biosynthesis, in regulating secondary vascular cambium activity in Populus. This study showed that the elevated endogenous castasterone (CS) levels in tree stems through overexpressing PagDET2 could enhance cambium meristem cell activity and xylem (XY) differentiation to promote secondary stem growth. RNA-seq analysis revealed that genes involved in BRs response, vascular cambium cell division, XY differentiation, and secondary cell wall synthesis were up-regulated.
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spelling pubmed-94592382022-09-10 PagDET2 promotes cambium cell division and xylem differentiation in poplar stem Wang, Yao Hao, Yi Guo, Yakun Shou, Huixia Du, Juan Front Plant Sci Plant Science Secondary growth of the woody tree stem is governed by meristematic cell division and differentiation in the vascular cambium. Multiple hormonal signals and endogenous developmental programs regulate vascular cambium activity. Brassinosteroids (BRs) significantly promote secondary stem growth and wood formation in poplar trees. However, the underlying regulatory mechanisms of BRs within the vascular tissue remain unclear. Genetic and anatomical approaches were used here to elucidate the role of PagDET2, the rate-limiting enzyme for BRs biosynthesis, in regulating secondary vascular cambium activity in Populus. This study showed that the elevated endogenous castasterone (CS) levels in tree stems through overexpressing PagDET2 could enhance cambium meristem cell activity and xylem (XY) differentiation to promote secondary stem growth. RNA-seq analysis revealed that genes involved in BRs response, vascular cambium cell division, XY differentiation, and secondary cell wall synthesis were up-regulated. Frontiers Media S.A. 2022-08-26 /pmc/articles/PMC9459238/ /pubmed/36092441 http://dx.doi.org/10.3389/fpls.2022.923530 Text en Copyright © 2022 Wang, Hao, Guo, Shou and Du. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wang, Yao
Hao, Yi
Guo, Yakun
Shou, Huixia
Du, Juan
PagDET2 promotes cambium cell division and xylem differentiation in poplar stem
title PagDET2 promotes cambium cell division and xylem differentiation in poplar stem
title_full PagDET2 promotes cambium cell division and xylem differentiation in poplar stem
title_fullStr PagDET2 promotes cambium cell division and xylem differentiation in poplar stem
title_full_unstemmed PagDET2 promotes cambium cell division and xylem differentiation in poplar stem
title_short PagDET2 promotes cambium cell division and xylem differentiation in poplar stem
title_sort pagdet2 promotes cambium cell division and xylem differentiation in poplar stem
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459238/
https://www.ncbi.nlm.nih.gov/pubmed/36092441
http://dx.doi.org/10.3389/fpls.2022.923530
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