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Consistency analysis of the Sysmex UF‐5000 and Atellica UAS 800 urine sedimentation analyzers

OBJECTIVE: To evaluate the consistency between the results of Sysmex UF‐5000 system and Atellica® UAS 800 Urine Sediment Analyzer. METHODS: A total of 636 random urine samples were collected from inpatients and outpatients from March to September 2021. Urine was collected for analysis by the Sysmex...

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Autores principales: Liu, Hai, Li, Qing, Zhang, Yiduo, Huang, Dongyue, Yu, Fan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459251/
https://www.ncbi.nlm.nih.gov/pubmed/35971777
http://dx.doi.org/10.1002/jcla.24659
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author Liu, Hai
Li, Qing
Zhang, Yiduo
Huang, Dongyue
Yu, Fan
author_facet Liu, Hai
Li, Qing
Zhang, Yiduo
Huang, Dongyue
Yu, Fan
author_sort Liu, Hai
collection PubMed
description OBJECTIVE: To evaluate the consistency between the results of Sysmex UF‐5000 system and Atellica® UAS 800 Urine Sediment Analyzer. METHODS: A total of 636 random urine samples were collected from inpatients and outpatients from March to September 2021. Urine was collected for analysis by the Sysmex UF‐5000, Atellica UAS 800 systems, and manual microscopic examination. The results of manual microscopy as the gold standard, the coincidence rate and false‐negative rate of Sysmex UF‐5000 and Atellica UAS 800 systems in the detection of red blood cells, white blood cells, and casts were calculated. RESULTS: The coincidence rates of red blood cells, white blood cells, and cast, crystals, and other sediment components for the Sysmex UF‐5000 system were 85.37%, 87.89%, 91.67%, 88.36%, and 71.86%. The false‐negative rates were 28.47%, 3.75%, 68.97%, 37.25%, and 30.63%. The coincidence rates of red blood cells, white blood cells, and cast, crystals, and other sediment components for the Atellica UAS 800 system were 85.06%, 90.25%, 59.12%, 91.67%, and 67.45% and the false‐negative rates were 60.42%, 21.25%, 36.21%, 19.64%, and 35.80%. CONCLUSION: Two instruments are superior in the detection of red blood cells and white blood cells. The Atellica UAS 800 system with image review has a good coincidence rate in the identification of crystals and casts. The identification of various sediment components in urine by both instruments meets the laboratory requirements. Two instruments with different methodologies have their own characteristics, and we should reasonably use them according to the conditions of the laboratory.
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spelling pubmed-94592512022-09-12 Consistency analysis of the Sysmex UF‐5000 and Atellica UAS 800 urine sedimentation analyzers Liu, Hai Li, Qing Zhang, Yiduo Huang, Dongyue Yu, Fan J Clin Lab Anal Research Articles OBJECTIVE: To evaluate the consistency between the results of Sysmex UF‐5000 system and Atellica® UAS 800 Urine Sediment Analyzer. METHODS: A total of 636 random urine samples were collected from inpatients and outpatients from March to September 2021. Urine was collected for analysis by the Sysmex UF‐5000, Atellica UAS 800 systems, and manual microscopic examination. The results of manual microscopy as the gold standard, the coincidence rate and false‐negative rate of Sysmex UF‐5000 and Atellica UAS 800 systems in the detection of red blood cells, white blood cells, and casts were calculated. RESULTS: The coincidence rates of red blood cells, white blood cells, and cast, crystals, and other sediment components for the Sysmex UF‐5000 system were 85.37%, 87.89%, 91.67%, 88.36%, and 71.86%. The false‐negative rates were 28.47%, 3.75%, 68.97%, 37.25%, and 30.63%. The coincidence rates of red blood cells, white blood cells, and cast, crystals, and other sediment components for the Atellica UAS 800 system were 85.06%, 90.25%, 59.12%, 91.67%, and 67.45% and the false‐negative rates were 60.42%, 21.25%, 36.21%, 19.64%, and 35.80%. CONCLUSION: Two instruments are superior in the detection of red blood cells and white blood cells. The Atellica UAS 800 system with image review has a good coincidence rate in the identification of crystals and casts. The identification of various sediment components in urine by both instruments meets the laboratory requirements. Two instruments with different methodologies have their own characteristics, and we should reasonably use them according to the conditions of the laboratory. John Wiley and Sons Inc. 2022-08-16 /pmc/articles/PMC9459251/ /pubmed/35971777 http://dx.doi.org/10.1002/jcla.24659 Text en © 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Liu, Hai
Li, Qing
Zhang, Yiduo
Huang, Dongyue
Yu, Fan
Consistency analysis of the Sysmex UF‐5000 and Atellica UAS 800 urine sedimentation analyzers
title Consistency analysis of the Sysmex UF‐5000 and Atellica UAS 800 urine sedimentation analyzers
title_full Consistency analysis of the Sysmex UF‐5000 and Atellica UAS 800 urine sedimentation analyzers
title_fullStr Consistency analysis of the Sysmex UF‐5000 and Atellica UAS 800 urine sedimentation analyzers
title_full_unstemmed Consistency analysis of the Sysmex UF‐5000 and Atellica UAS 800 urine sedimentation analyzers
title_short Consistency analysis of the Sysmex UF‐5000 and Atellica UAS 800 urine sedimentation analyzers
title_sort consistency analysis of the sysmex uf‐5000 and atellica uas 800 urine sedimentation analyzers
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459251/
https://www.ncbi.nlm.nih.gov/pubmed/35971777
http://dx.doi.org/10.1002/jcla.24659
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