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An insight into the diagnostic and prognostic value of HOX A13’s expression in non‐muscle invasive bladder cancer
BACKGROUND: Several studies have interrogated the molecular pathways and their interacting genes underlying bladder cancer (BCa) tumorigenesis, yet, the role of homeobox genes is still poorly understood. Specifically, HOXA13, which plays an important role as a major actor in the urogenital tract...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459288/ https://www.ncbi.nlm.nih.gov/pubmed/35853090 http://dx.doi.org/10.1002/jcla.24606 |
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author | Setti Boubaker, Nouha Gurtner, Aymone Trabelsi, Nesrine Manni, Isabella Blel, Ahlem Saadi, Ahmed Chakroun, Marouene Naimi, Zeineb Zaghbib, Selim Ksontini, Meriam Meddeb, Khedija Rammeh, Soumaya Ayed, Haroun Chebil, Mohamed Piaggio, Giulia Ouerhani, Slah |
author_facet | Setti Boubaker, Nouha Gurtner, Aymone Trabelsi, Nesrine Manni, Isabella Blel, Ahlem Saadi, Ahmed Chakroun, Marouene Naimi, Zeineb Zaghbib, Selim Ksontini, Meriam Meddeb, Khedija Rammeh, Soumaya Ayed, Haroun Chebil, Mohamed Piaggio, Giulia Ouerhani, Slah |
author_sort | Setti Boubaker, Nouha |
collection | PubMed |
description | BACKGROUND: Several studies have interrogated the molecular pathways and their interacting genes underlying bladder cancer (BCa) tumorigenesis, yet, the role of homeobox genes is still poorly understood. Specifically, HOXA13, which plays an important role as a major actor in the urogenital tract's development. METHODS: Immunohistochemical (IHC) staining was performed to inspect the differential expression of HOXA13 protein in non‐muscle‐invasive bladder cancer (NMIBC) and non‐tumoral tissues. A semiquantitative scoring system was adopted to evaluate the IHC labeling. Correlation to clinical parameters was performed by descriptive statistics. Overall survival was estimated by the Kaplan–Meier method and Cox regression model. The functional HOX A13 protein association networks (PPI) were obtained using String 11.0 database. RESULTS: HOX A13 exhibited cytoplasmic and nuclear staining. Its expression levels were lower in high‐grade NMIBC (HG NMIBC) compared to low‐grade ones (LG NMIBC). The expression of HOX A13 was correlated to tumor grade (LG/HG) (p = 0.036) and stage (TA/T1) (p = 0.036). Nevertheless, its expression was not correlated to clinical parameters and was not able to predict the overall survival of patients with HG NMIBC. Finally, PPI analysis revealed that HOX A13 seems to be a part of a molecular network holding mainly PBX1, MEIS, ALDH1A2, HOX A10, and HOX A11. CONCLUSION: The deregulation of HOX A13 is not associated with the prognosis of BCa. It seems to be rather implicated in the early initiation of urothelial tumorigenesis and thus may serve as a diagnostic marker in patients with NMIBC. Further experimentations on larger validation sets are mandatory. |
format | Online Article Text |
id | pubmed-9459288 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94592882022-09-12 An insight into the diagnostic and prognostic value of HOX A13’s expression in non‐muscle invasive bladder cancer Setti Boubaker, Nouha Gurtner, Aymone Trabelsi, Nesrine Manni, Isabella Blel, Ahlem Saadi, Ahmed Chakroun, Marouene Naimi, Zeineb Zaghbib, Selim Ksontini, Meriam Meddeb, Khedija Rammeh, Soumaya Ayed, Haroun Chebil, Mohamed Piaggio, Giulia Ouerhani, Slah J Clin Lab Anal Research Articles BACKGROUND: Several studies have interrogated the molecular pathways and their interacting genes underlying bladder cancer (BCa) tumorigenesis, yet, the role of homeobox genes is still poorly understood. Specifically, HOXA13, which plays an important role as a major actor in the urogenital tract's development. METHODS: Immunohistochemical (IHC) staining was performed to inspect the differential expression of HOXA13 protein in non‐muscle‐invasive bladder cancer (NMIBC) and non‐tumoral tissues. A semiquantitative scoring system was adopted to evaluate the IHC labeling. Correlation to clinical parameters was performed by descriptive statistics. Overall survival was estimated by the Kaplan–Meier method and Cox regression model. The functional HOX A13 protein association networks (PPI) were obtained using String 11.0 database. RESULTS: HOX A13 exhibited cytoplasmic and nuclear staining. Its expression levels were lower in high‐grade NMIBC (HG NMIBC) compared to low‐grade ones (LG NMIBC). The expression of HOX A13 was correlated to tumor grade (LG/HG) (p = 0.036) and stage (TA/T1) (p = 0.036). Nevertheless, its expression was not correlated to clinical parameters and was not able to predict the overall survival of patients with HG NMIBC. Finally, PPI analysis revealed that HOX A13 seems to be a part of a molecular network holding mainly PBX1, MEIS, ALDH1A2, HOX A10, and HOX A11. CONCLUSION: The deregulation of HOX A13 is not associated with the prognosis of BCa. It seems to be rather implicated in the early initiation of urothelial tumorigenesis and thus may serve as a diagnostic marker in patients with NMIBC. Further experimentations on larger validation sets are mandatory. John Wiley and Sons Inc. 2022-07-19 /pmc/articles/PMC9459288/ /pubmed/35853090 http://dx.doi.org/10.1002/jcla.24606 Text en © 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Setti Boubaker, Nouha Gurtner, Aymone Trabelsi, Nesrine Manni, Isabella Blel, Ahlem Saadi, Ahmed Chakroun, Marouene Naimi, Zeineb Zaghbib, Selim Ksontini, Meriam Meddeb, Khedija Rammeh, Soumaya Ayed, Haroun Chebil, Mohamed Piaggio, Giulia Ouerhani, Slah An insight into the diagnostic and prognostic value of HOX A13’s expression in non‐muscle invasive bladder cancer |
title | An insight into the diagnostic and prognostic value of HOX A13’s expression in non‐muscle invasive bladder cancer |
title_full | An insight into the diagnostic and prognostic value of HOX A13’s expression in non‐muscle invasive bladder cancer |
title_fullStr | An insight into the diagnostic and prognostic value of HOX A13’s expression in non‐muscle invasive bladder cancer |
title_full_unstemmed | An insight into the diagnostic and prognostic value of HOX A13’s expression in non‐muscle invasive bladder cancer |
title_short | An insight into the diagnostic and prognostic value of HOX A13’s expression in non‐muscle invasive bladder cancer |
title_sort | insight into the diagnostic and prognostic value of hox a13’s expression in non‐muscle invasive bladder cancer |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459288/ https://www.ncbi.nlm.nih.gov/pubmed/35853090 http://dx.doi.org/10.1002/jcla.24606 |
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