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TIFA promotes colorectal cancer cell proliferation in an RSK‐ and PRAS40‐dependent manner

Previous studies have reported that TIFA plays different roles in various tumor types. However, the function of TIFA in colorectal cancer (CRC) remains unclear. Here, we showed that the expression of TIFA was markedly increased in CRC versus normal tissue, and positively correlated with CRC TNM stag...

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Autores principales: Shen, Wenzhi, Du, Wenfei, Li, Yanping, Huang, Yongming, Jiang, Xinyu, Yang, Chenglong, Tang, Jiaping, Liu, Huan, Luo, Na, Zhang, Xiaoyuan, Zhang, Zhixin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459298/
https://www.ncbi.nlm.nih.gov/pubmed/35635239
http://dx.doi.org/10.1111/cas.15432
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author Shen, Wenzhi
Du, Wenfei
Li, Yanping
Huang, Yongming
Jiang, Xinyu
Yang, Chenglong
Tang, Jiaping
Liu, Huan
Luo, Na
Zhang, Xiaoyuan
Zhang, Zhixin
author_facet Shen, Wenzhi
Du, Wenfei
Li, Yanping
Huang, Yongming
Jiang, Xinyu
Yang, Chenglong
Tang, Jiaping
Liu, Huan
Luo, Na
Zhang, Xiaoyuan
Zhang, Zhixin
author_sort Shen, Wenzhi
collection PubMed
description Previous studies have reported that TIFA plays different roles in various tumor types. However, the function of TIFA in colorectal cancer (CRC) remains unclear. Here, we showed that the expression of TIFA was markedly increased in CRC versus normal tissue, and positively correlated with CRC TNM stages. In agreement, we found that the CRC cell lines show increased TIFA expression levels versus normal control. The knockdown of TIFA inhibited cell proliferation but had no effect on cell apoptosis in vitro or in vivo. Moreover, the ectopic expression of TIFA enhanced cell proliferation ability in vitro and in vivo. In contrast, the expression of mutant TIFA (T9A, oligomerization site mutation; D6, TRAF6 binding site deletion) abolished TIFA‐mediated cell proliferation enhancement. Exploration of the underlying mechanism revealed that the protein synthesis‐associated kinase RSK and PRAS40 activation were responsible for TIFA‐mediated CRC progression. In summary, these findings suggest that TIFA plays a role in mediating CRC progression. This could provide a promising target for CRC therapy.
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spelling pubmed-94592982022-09-12 TIFA promotes colorectal cancer cell proliferation in an RSK‐ and PRAS40‐dependent manner Shen, Wenzhi Du, Wenfei Li, Yanping Huang, Yongming Jiang, Xinyu Yang, Chenglong Tang, Jiaping Liu, Huan Luo, Na Zhang, Xiaoyuan Zhang, Zhixin Cancer Sci Original Articles Previous studies have reported that TIFA plays different roles in various tumor types. However, the function of TIFA in colorectal cancer (CRC) remains unclear. Here, we showed that the expression of TIFA was markedly increased in CRC versus normal tissue, and positively correlated with CRC TNM stages. In agreement, we found that the CRC cell lines show increased TIFA expression levels versus normal control. The knockdown of TIFA inhibited cell proliferation but had no effect on cell apoptosis in vitro or in vivo. Moreover, the ectopic expression of TIFA enhanced cell proliferation ability in vitro and in vivo. In contrast, the expression of mutant TIFA (T9A, oligomerization site mutation; D6, TRAF6 binding site deletion) abolished TIFA‐mediated cell proliferation enhancement. Exploration of the underlying mechanism revealed that the protein synthesis‐associated kinase RSK and PRAS40 activation were responsible for TIFA‐mediated CRC progression. In summary, these findings suggest that TIFA plays a role in mediating CRC progression. This could provide a promising target for CRC therapy. John Wiley and Sons Inc. 2022-06-14 2022-09 /pmc/articles/PMC9459298/ /pubmed/35635239 http://dx.doi.org/10.1111/cas.15432 Text en © 2022 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Shen, Wenzhi
Du, Wenfei
Li, Yanping
Huang, Yongming
Jiang, Xinyu
Yang, Chenglong
Tang, Jiaping
Liu, Huan
Luo, Na
Zhang, Xiaoyuan
Zhang, Zhixin
TIFA promotes colorectal cancer cell proliferation in an RSK‐ and PRAS40‐dependent manner
title TIFA promotes colorectal cancer cell proliferation in an RSK‐ and PRAS40‐dependent manner
title_full TIFA promotes colorectal cancer cell proliferation in an RSK‐ and PRAS40‐dependent manner
title_fullStr TIFA promotes colorectal cancer cell proliferation in an RSK‐ and PRAS40‐dependent manner
title_full_unstemmed TIFA promotes colorectal cancer cell proliferation in an RSK‐ and PRAS40‐dependent manner
title_short TIFA promotes colorectal cancer cell proliferation in an RSK‐ and PRAS40‐dependent manner
title_sort tifa promotes colorectal cancer cell proliferation in an rsk‐ and pras40‐dependent manner
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459298/
https://www.ncbi.nlm.nih.gov/pubmed/35635239
http://dx.doi.org/10.1111/cas.15432
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