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Phenotype, genotype, and mating type determination in oral Candida albicans isolates from pediatric patients with neutropenia
BACKGROUND: The most frequent species of Candida to infect and colonize patients with neutropenia is still Candida albicans. This study aimed to provide detailed information on the phenotype, genotype, and mating type of oral C. albicans isolated from neutropenic pediatric patients, and to investiga...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459309/ https://www.ncbi.nlm.nih.gov/pubmed/36082467 http://dx.doi.org/10.1002/jcla.24664 |
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author | Jafarian, Hadis Gharaghani, Maral Asnafi, Ali Amin Hardani, Amir Kamal Zarei‐Mahmoudabadi, Ali |
author_facet | Jafarian, Hadis Gharaghani, Maral Asnafi, Ali Amin Hardani, Amir Kamal Zarei‐Mahmoudabadi, Ali |
author_sort | Jafarian, Hadis |
collection | PubMed |
description | BACKGROUND: The most frequent species of Candida to infect and colonize patients with neutropenia is still Candida albicans. This study aimed to provide detailed information on the phenotype, genotype, and mating type of oral C. albicans isolated from neutropenic pediatric patients, and to investigate how these characteristics are related. METHODS: Two hundred fifty‐four oral samples from patients under 18 years old with neutropenia and malignancies were collected from January to October 2021. Samples were cultured on CHROMagar Candida. Isolates of C. albicans were identified with the germ tube test, chlamydospore production on cornmeal agar, and PCR‐RFLP. Genotyping of C. albicans isolates was carried out by amplifying the 25S rDNA gene with specific CAINT‐L and CA‐INT‐R primers. MTLa1 and MTLα1 primers were used to identify each mating type. Yeast peptone dextrose supplemented with phloxine B was used to identify different phenotypes. RESULTS: Ninety‐two (36%) patients were positive for C. albicans. The mean age of patients was 7.85. Fifty‐three (58.9%) isolates demonstrated type A, 15 (16.7%) type B, 15 (16.7%) types D/E, and 7 (7.7%) type C. Three isolates each (3.3%) were homozygous for MTLa or homozygous for MTLα. All of the MTL‐homozygous isolates were genotype A. There was a significant correlation between patients' underlying disease and genotype (p = 0.036). There was a significant correlation between mating type and genotype (p = 0.000). CONCLUSION: Most of the isolates exhibited a white phenotype, noted in the literature as the most virulent. Moreover, heterozygous strains were frequent and may play a role in Candida colonization. |
format | Online Article Text |
id | pubmed-9459309 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94593092022-09-12 Phenotype, genotype, and mating type determination in oral Candida albicans isolates from pediatric patients with neutropenia Jafarian, Hadis Gharaghani, Maral Asnafi, Ali Amin Hardani, Amir Kamal Zarei‐Mahmoudabadi, Ali J Clin Lab Anal Research Articles BACKGROUND: The most frequent species of Candida to infect and colonize patients with neutropenia is still Candida albicans. This study aimed to provide detailed information on the phenotype, genotype, and mating type of oral C. albicans isolated from neutropenic pediatric patients, and to investigate how these characteristics are related. METHODS: Two hundred fifty‐four oral samples from patients under 18 years old with neutropenia and malignancies were collected from January to October 2021. Samples were cultured on CHROMagar Candida. Isolates of C. albicans were identified with the germ tube test, chlamydospore production on cornmeal agar, and PCR‐RFLP. Genotyping of C. albicans isolates was carried out by amplifying the 25S rDNA gene with specific CAINT‐L and CA‐INT‐R primers. MTLa1 and MTLα1 primers were used to identify each mating type. Yeast peptone dextrose supplemented with phloxine B was used to identify different phenotypes. RESULTS: Ninety‐two (36%) patients were positive for C. albicans. The mean age of patients was 7.85. Fifty‐three (58.9%) isolates demonstrated type A, 15 (16.7%) type B, 15 (16.7%) types D/E, and 7 (7.7%) type C. Three isolates each (3.3%) were homozygous for MTLa or homozygous for MTLα. All of the MTL‐homozygous isolates were genotype A. There was a significant correlation between patients' underlying disease and genotype (p = 0.036). There was a significant correlation between mating type and genotype (p = 0.000). CONCLUSION: Most of the isolates exhibited a white phenotype, noted in the literature as the most virulent. Moreover, heterozygous strains were frequent and may play a role in Candida colonization. John Wiley and Sons Inc. 2022-08-16 /pmc/articles/PMC9459309/ /pubmed/36082467 http://dx.doi.org/10.1002/jcla.24664 Text en © 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Jafarian, Hadis Gharaghani, Maral Asnafi, Ali Amin Hardani, Amir Kamal Zarei‐Mahmoudabadi, Ali Phenotype, genotype, and mating type determination in oral Candida albicans isolates from pediatric patients with neutropenia |
title | Phenotype, genotype, and mating type determination in oral Candida albicans isolates from pediatric patients with neutropenia |
title_full | Phenotype, genotype, and mating type determination in oral Candida albicans isolates from pediatric patients with neutropenia |
title_fullStr | Phenotype, genotype, and mating type determination in oral Candida albicans isolates from pediatric patients with neutropenia |
title_full_unstemmed | Phenotype, genotype, and mating type determination in oral Candida albicans isolates from pediatric patients with neutropenia |
title_short | Phenotype, genotype, and mating type determination in oral Candida albicans isolates from pediatric patients with neutropenia |
title_sort | phenotype, genotype, and mating type determination in oral candida albicans isolates from pediatric patients with neutropenia |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459309/ https://www.ncbi.nlm.nih.gov/pubmed/36082467 http://dx.doi.org/10.1002/jcla.24664 |
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