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Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheep

Silage is rich in nutrients, which can make up for the lack of seasonal roughage, and has a certain promotion effect on the intensive feeding of ruminants. In addition, silage can maintain the rumen function of ruminants to a certain extent and reduce the risk of rumen acidosis and abomasum transloc...

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Autores principales: Li, Chuang, Chen, Ning, Zhang, Xingxing, Shahzad, Khuram, Qi, Ruxin, Zhang, Zhenbin, Lu, Zhiqi, Lu, Yue, Yu, Xiang, Zafar, Muhammad Hammad, Wang, Mengzhi, Liu, Wujun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459383/
https://www.ncbi.nlm.nih.gov/pubmed/36090065
http://dx.doi.org/10.3389/fmicb.2022.978940
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author Li, Chuang
Chen, Ning
Zhang, Xingxing
Shahzad, Khuram
Qi, Ruxin
Zhang, Zhenbin
Lu, Zhiqi
Lu, Yue
Yu, Xiang
Zafar, Muhammad Hammad
Wang, Mengzhi
Liu, Wujun
author_facet Li, Chuang
Chen, Ning
Zhang, Xingxing
Shahzad, Khuram
Qi, Ruxin
Zhang, Zhenbin
Lu, Zhiqi
Lu, Yue
Yu, Xiang
Zafar, Muhammad Hammad
Wang, Mengzhi
Liu, Wujun
author_sort Li, Chuang
collection PubMed
description Silage is rich in nutrients, which can make up for the lack of seasonal roughage, and has a certain promotion effect on the intensive feeding of ruminants. In addition, silage can maintain the rumen function of ruminants to a certain extent and reduce the risk of rumen acidosis and abomasum translocation. The purpose of this study was to investigate the effects of the mixed silage of Chinese cabbage waste and rice straw (mixed silage) on antioxidant performance, rumen microbial population, and fermentation metabolism of Hu sheep. The 16 healthy Hu sheep (eight rams and eight ewes, 39.11 ± 1.16 kg, 5.5 months) were randomly divided into two groups (the control group and the mixed silage group) with eight animals (four rams and four ewes) in each group. The control group was fed with farm roughage (peanut seedlings, corn husk, and high grain shell) as forage, and the mixed silage group was fed with the mixed silage as forage. The results showed that the mixed silage had no effect on the growth performance of Hu sheep (p > 0.05). Ruminal butyric acid, total volatile fatty acids (TVFA), and ammonia nitrogen (NH(3)-N) concentration in the mixed silage group were increased, whereas the pH was decreased (p < 0.05). The blood and rumen total antioxidants capacity (T-AOC) concentration in the mixed silage group was higher, and the malondialdehyde (MDA) content in rumen, serum, liver, and kidney was lower than that in the control group (p < 0.05). PCoA and ANOSIM results of Illumina sequencing indicated that the mixed silage affected the bacterial composition of the rumen microbes. The mixed silage increased the proportion of Prevotellaceae UCG-004 which was in a positive correlation with Vitamin C (Vc). In addition, PICRUSt functional prediction analysis showed that ascorbate and aldarate metabolism were up-regulated in the mixed silage group (p < 0.05). In conclusion, higher contents of V(C) and acid detergent fiber (ADF) in the mixed silage were beneficial to the growth and reproduction of Prevotellaceae UCG-004, resulting in increased production of the butyric acid significantly upregulated the metabolism of ascorbate and aldarate metabolism, thereby improving the antioxidant properties of Hu sheep.
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spelling pubmed-94593832022-09-10 Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheep Li, Chuang Chen, Ning Zhang, Xingxing Shahzad, Khuram Qi, Ruxin Zhang, Zhenbin Lu, Zhiqi Lu, Yue Yu, Xiang Zafar, Muhammad Hammad Wang, Mengzhi Liu, Wujun Front Microbiol Microbiology Silage is rich in nutrients, which can make up for the lack of seasonal roughage, and has a certain promotion effect on the intensive feeding of ruminants. In addition, silage can maintain the rumen function of ruminants to a certain extent and reduce the risk of rumen acidosis and abomasum translocation. The purpose of this study was to investigate the effects of the mixed silage of Chinese cabbage waste and rice straw (mixed silage) on antioxidant performance, rumen microbial population, and fermentation metabolism of Hu sheep. The 16 healthy Hu sheep (eight rams and eight ewes, 39.11 ± 1.16 kg, 5.5 months) were randomly divided into two groups (the control group and the mixed silage group) with eight animals (four rams and four ewes) in each group. The control group was fed with farm roughage (peanut seedlings, corn husk, and high grain shell) as forage, and the mixed silage group was fed with the mixed silage as forage. The results showed that the mixed silage had no effect on the growth performance of Hu sheep (p > 0.05). Ruminal butyric acid, total volatile fatty acids (TVFA), and ammonia nitrogen (NH(3)-N) concentration in the mixed silage group were increased, whereas the pH was decreased (p < 0.05). The blood and rumen total antioxidants capacity (T-AOC) concentration in the mixed silage group was higher, and the malondialdehyde (MDA) content in rumen, serum, liver, and kidney was lower than that in the control group (p < 0.05). PCoA and ANOSIM results of Illumina sequencing indicated that the mixed silage affected the bacterial composition of the rumen microbes. The mixed silage increased the proportion of Prevotellaceae UCG-004 which was in a positive correlation with Vitamin C (Vc). In addition, PICRUSt functional prediction analysis showed that ascorbate and aldarate metabolism were up-regulated in the mixed silage group (p < 0.05). In conclusion, higher contents of V(C) and acid detergent fiber (ADF) in the mixed silage were beneficial to the growth and reproduction of Prevotellaceae UCG-004, resulting in increased production of the butyric acid significantly upregulated the metabolism of ascorbate and aldarate metabolism, thereby improving the antioxidant properties of Hu sheep. Frontiers Media S.A. 2022-08-26 /pmc/articles/PMC9459383/ /pubmed/36090065 http://dx.doi.org/10.3389/fmicb.2022.978940 Text en Copyright © 2022 Li, Chen, Zhang, Shahzad, Qi, Zhang, Lu, Lu, Yu, Zafar, Wang and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Chuang
Chen, Ning
Zhang, Xingxing
Shahzad, Khuram
Qi, Ruxin
Zhang, Zhenbin
Lu, Zhiqi
Lu, Yue
Yu, Xiang
Zafar, Muhammad Hammad
Wang, Mengzhi
Liu, Wujun
Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheep
title Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheep
title_full Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheep
title_fullStr Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheep
title_full_unstemmed Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheep
title_short Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheep
title_sort mixed silage with chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen prevotellaceae ucg-004 in hu sheep
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459383/
https://www.ncbi.nlm.nih.gov/pubmed/36090065
http://dx.doi.org/10.3389/fmicb.2022.978940
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