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Validity of serum and urinary hepcidin as biomarkers for late-onset sepsis in premature infants

BACKGROUND: Sepsis remains one of the leading causes of neonatal morbidity and mortality, particularly among premature infants. Blood culture is the ‘gold standard’ for the diagnosis of neonatal sepsis but is associated with several pitfalls. AIM OF THE WORK: We aim to evaluate the validity of measu...

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Autores principales: Sherbiny, Hanan Sakr, Mostafa, Hanaa Abdel-el Fattah, Sherief, Laila M., Kamal, Naglaa M., El-Shal, Amal Saeed, Abdel-el Halm, Mahmoud Mohamed, Khan, Hekmat Yaqoub, Ali, Al Shaymaa Ahmed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459492/
https://www.ncbi.nlm.nih.gov/pubmed/36093263
http://dx.doi.org/10.1177/20406223221122527
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author Sherbiny, Hanan Sakr
Mostafa, Hanaa Abdel-el Fattah
Sherief, Laila M.
Kamal, Naglaa M.
El-Shal, Amal Saeed
Abdel-el Halm, Mahmoud Mohamed
Khan, Hekmat Yaqoub
Ali, Al Shaymaa Ahmed
author_facet Sherbiny, Hanan Sakr
Mostafa, Hanaa Abdel-el Fattah
Sherief, Laila M.
Kamal, Naglaa M.
El-Shal, Amal Saeed
Abdel-el Halm, Mahmoud Mohamed
Khan, Hekmat Yaqoub
Ali, Al Shaymaa Ahmed
author_sort Sherbiny, Hanan Sakr
collection PubMed
description BACKGROUND: Sepsis remains one of the leading causes of neonatal morbidity and mortality, particularly among premature infants. Blood culture is the ‘gold standard’ for the diagnosis of neonatal sepsis but is associated with several pitfalls. AIM OF THE WORK: We aim to evaluate the validity of measuring serum (S.Hep) and urinary hepcidin (U.Hep) concentrations as diagnostic biomarkers for late-onset sepsis (LOS) in preterm infants. PATIENTS AND METHODS: The current case-control study included 73 cases of clinically and laboratory confirmed late-onset sepsis as the ‘case group’ and 50 nonseptic premature infants of comparable age and sex as the ‘control group’. S.Hep and U.Hep concentrations were evaluated at enrollment (acute sample) and after 1 week of treatment (convalescent sample). RESULTS: Patients had a statistically significant higher concentration of both S.Hep and U.Hep as compared with nonseptic controls (p = 0.0001). Similarly, a significant reduction of both S.Hep and U.Hep was detected after 1 week of treatment (p = 0.001). A cut-off value of ⩾ 94.8 ng/ml of S.Hep and ⩾ 264 ng/mg of U.Hep/urinary creatinine showed high sensitivity, specificity, and positive predictive value in the diagnosis of neonatal LOS. CONCLUSIONS: Both S.Hep and U.Hep can function as promising accurate and rapid surrogate tests for the diagnosis of LOS. U.Hep measurement has the advantage of being noninvasive, with no hazards of phlebotomy, and is less variable throughout the day.
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spelling pubmed-94594922022-09-10 Validity of serum and urinary hepcidin as biomarkers for late-onset sepsis in premature infants Sherbiny, Hanan Sakr Mostafa, Hanaa Abdel-el Fattah Sherief, Laila M. Kamal, Naglaa M. El-Shal, Amal Saeed Abdel-el Halm, Mahmoud Mohamed Khan, Hekmat Yaqoub Ali, Al Shaymaa Ahmed Ther Adv Chronic Dis Original Research BACKGROUND: Sepsis remains one of the leading causes of neonatal morbidity and mortality, particularly among premature infants. Blood culture is the ‘gold standard’ for the diagnosis of neonatal sepsis but is associated with several pitfalls. AIM OF THE WORK: We aim to evaluate the validity of measuring serum (S.Hep) and urinary hepcidin (U.Hep) concentrations as diagnostic biomarkers for late-onset sepsis (LOS) in preterm infants. PATIENTS AND METHODS: The current case-control study included 73 cases of clinically and laboratory confirmed late-onset sepsis as the ‘case group’ and 50 nonseptic premature infants of comparable age and sex as the ‘control group’. S.Hep and U.Hep concentrations were evaluated at enrollment (acute sample) and after 1 week of treatment (convalescent sample). RESULTS: Patients had a statistically significant higher concentration of both S.Hep and U.Hep as compared with nonseptic controls (p = 0.0001). Similarly, a significant reduction of both S.Hep and U.Hep was detected after 1 week of treatment (p = 0.001). A cut-off value of ⩾ 94.8 ng/ml of S.Hep and ⩾ 264 ng/mg of U.Hep/urinary creatinine showed high sensitivity, specificity, and positive predictive value in the diagnosis of neonatal LOS. CONCLUSIONS: Both S.Hep and U.Hep can function as promising accurate and rapid surrogate tests for the diagnosis of LOS. U.Hep measurement has the advantage of being noninvasive, with no hazards of phlebotomy, and is less variable throughout the day. SAGE Publications 2022-09-06 /pmc/articles/PMC9459492/ /pubmed/36093263 http://dx.doi.org/10.1177/20406223221122527 Text en © The Author(s), 2022 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Research
Sherbiny, Hanan Sakr
Mostafa, Hanaa Abdel-el Fattah
Sherief, Laila M.
Kamal, Naglaa M.
El-Shal, Amal Saeed
Abdel-el Halm, Mahmoud Mohamed
Khan, Hekmat Yaqoub
Ali, Al Shaymaa Ahmed
Validity of serum and urinary hepcidin as biomarkers for late-onset sepsis in premature infants
title Validity of serum and urinary hepcidin as biomarkers for late-onset sepsis in premature infants
title_full Validity of serum and urinary hepcidin as biomarkers for late-onset sepsis in premature infants
title_fullStr Validity of serum and urinary hepcidin as biomarkers for late-onset sepsis in premature infants
title_full_unstemmed Validity of serum and urinary hepcidin as biomarkers for late-onset sepsis in premature infants
title_short Validity of serum and urinary hepcidin as biomarkers for late-onset sepsis in premature infants
title_sort validity of serum and urinary hepcidin as biomarkers for late-onset sepsis in premature infants
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459492/
https://www.ncbi.nlm.nih.gov/pubmed/36093263
http://dx.doi.org/10.1177/20406223221122527
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