Cargando…

Effects of gold nanoparticles on normal hepatocytes in radiation therapy

BACKGROUND: Gold nanoparticles (GNP, AuNPs) have received much attention as a tool to improve the therapeutic index of radiation therapy. This study aimed to evaluate the normal in vitro toxicity of AuNPs at kilovoltage energies on hepatocytes to provide scientific support for using AuNPs with radio...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Jeong-Yub, Lee, Won Seok, Seo, Seung-Jun, Jung, Chan-Woong, Kim, Eun Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459506/
https://www.ncbi.nlm.nih.gov/pubmed/36093516
http://dx.doi.org/10.21037/tcr-21-1855
_version_ 1784786526096326656
author Kim, Jeong-Yub
Lee, Won Seok
Seo, Seung-Jun
Jung, Chan-Woong
Kim, Eun Ho
author_facet Kim, Jeong-Yub
Lee, Won Seok
Seo, Seung-Jun
Jung, Chan-Woong
Kim, Eun Ho
author_sort Kim, Jeong-Yub
collection PubMed
description BACKGROUND: Gold nanoparticles (GNP, AuNPs) have received much attention as a tool to improve the therapeutic index of radiation therapy. This study aimed to evaluate the normal in vitro toxicity of AuNPs at kilovoltage energies on hepatocytes to provide scientific support for using AuNPs with radiotherapy. METHODS: Using the same treatment protocol applied to tumor cell lines, hepatocytes were exposed to AuNPs and/or radiation at various time points. RESULTS: The combination of X-ray irradiation and AuNPs did not have any significant effect on cell survival and apoptosis in normal hepatocytes. Furthermore, the combination treatment resulted in no or little change in the level of gamma-H2A histone family member X (γ-H2AX), a marker for DNA double-strand breaks (DSB), nor on the proportion of cells in the G2/M phase. Additionally, interleukin-8 (IL-8) secretion was measured using an enzyme-linked immunosorbent assay (ELISA) to assess its role in tumor progression and angiogenesis. The combination of irradiation and AuNP treatment revealed no significant reduction in hepatocyte viability, proliferation, or secretory capacity compared to cells receiving either treatment alone. According to this study, AuNPs in combination with radiation do have potentially in the treatment of hepatocellular carcinoma (HCC) with no critical cytotoxicity on normal tissue. CONCLUSIONS: Therefore, it is postulated that radiation and AuNPs are an effective combination therapy against HCC with no little cytotoxic effects on normal tissue, a hypothesis which warrants further investigation in in vivo, as well as in in vitro.
format Online
Article
Text
id pubmed-9459506
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher AME Publishing Company
record_format MEDLINE/PubMed
spelling pubmed-94595062022-09-10 Effects of gold nanoparticles on normal hepatocytes in radiation therapy Kim, Jeong-Yub Lee, Won Seok Seo, Seung-Jun Jung, Chan-Woong Kim, Eun Ho Transl Cancer Res Original Article BACKGROUND: Gold nanoparticles (GNP, AuNPs) have received much attention as a tool to improve the therapeutic index of radiation therapy. This study aimed to evaluate the normal in vitro toxicity of AuNPs at kilovoltage energies on hepatocytes to provide scientific support for using AuNPs with radiotherapy. METHODS: Using the same treatment protocol applied to tumor cell lines, hepatocytes were exposed to AuNPs and/or radiation at various time points. RESULTS: The combination of X-ray irradiation and AuNPs did not have any significant effect on cell survival and apoptosis in normal hepatocytes. Furthermore, the combination treatment resulted in no or little change in the level of gamma-H2A histone family member X (γ-H2AX), a marker for DNA double-strand breaks (DSB), nor on the proportion of cells in the G2/M phase. Additionally, interleukin-8 (IL-8) secretion was measured using an enzyme-linked immunosorbent assay (ELISA) to assess its role in tumor progression and angiogenesis. The combination of irradiation and AuNP treatment revealed no significant reduction in hepatocyte viability, proliferation, or secretory capacity compared to cells receiving either treatment alone. According to this study, AuNPs in combination with radiation do have potentially in the treatment of hepatocellular carcinoma (HCC) with no critical cytotoxicity on normal tissue. CONCLUSIONS: Therefore, it is postulated that radiation and AuNPs are an effective combination therapy against HCC with no little cytotoxic effects on normal tissue, a hypothesis which warrants further investigation in in vivo, as well as in in vitro. AME Publishing Company 2022-08 /pmc/articles/PMC9459506/ /pubmed/36093516 http://dx.doi.org/10.21037/tcr-21-1855 Text en 2022 Translational Cancer Research. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Kim, Jeong-Yub
Lee, Won Seok
Seo, Seung-Jun
Jung, Chan-Woong
Kim, Eun Ho
Effects of gold nanoparticles on normal hepatocytes in radiation therapy
title Effects of gold nanoparticles on normal hepatocytes in radiation therapy
title_full Effects of gold nanoparticles on normal hepatocytes in radiation therapy
title_fullStr Effects of gold nanoparticles on normal hepatocytes in radiation therapy
title_full_unstemmed Effects of gold nanoparticles on normal hepatocytes in radiation therapy
title_short Effects of gold nanoparticles on normal hepatocytes in radiation therapy
title_sort effects of gold nanoparticles on normal hepatocytes in radiation therapy
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9459506/
https://www.ncbi.nlm.nih.gov/pubmed/36093516
http://dx.doi.org/10.21037/tcr-21-1855
work_keys_str_mv AT kimjeongyub effectsofgoldnanoparticlesonnormalhepatocytesinradiationtherapy
AT leewonseok effectsofgoldnanoparticlesonnormalhepatocytesinradiationtherapy
AT seoseungjun effectsofgoldnanoparticlesonnormalhepatocytesinradiationtherapy
AT jungchanwoong effectsofgoldnanoparticlesonnormalhepatocytesinradiationtherapy
AT kimeunho effectsofgoldnanoparticlesonnormalhepatocytesinradiationtherapy