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Effects of the Long-Term Consumption of a High-Sucrose Diet on microRNA Expression in Visceral Adipose Tissue of Rats

Noncoding microRNAs are involved in lipid and carbohydrate metabolism pathways and are powerful regulators of gene expression. The goals of this study were to evaluate the temporal expression profiles of miRNAs in rat adipose tissue and predict mRNA–microRNA interactions. Newly weaned Wistar rats we...

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Autores principales: Fernandes, Isabela Costa, Santos, Talita Adriana Pereira, Oliveira, Daiane Teixeira, Oliveira, Victor Fernandes, Sousa, Graziele Galdino, Pereira, Luciene Santos, Barboza, Natália Rocha, Evangelista, Elísio Alberto, Guerra-Sá, Renata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9460050/
https://www.ncbi.nlm.nih.gov/pubmed/36079722
http://dx.doi.org/10.3390/nu14173465
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author Fernandes, Isabela Costa
Santos, Talita Adriana Pereira
Oliveira, Daiane Teixeira
Oliveira, Victor Fernandes
Sousa, Graziele Galdino
Pereira, Luciene Santos
Barboza, Natália Rocha
Evangelista, Elísio Alberto
Guerra-Sá, Renata
author_facet Fernandes, Isabela Costa
Santos, Talita Adriana Pereira
Oliveira, Daiane Teixeira
Oliveira, Victor Fernandes
Sousa, Graziele Galdino
Pereira, Luciene Santos
Barboza, Natália Rocha
Evangelista, Elísio Alberto
Guerra-Sá, Renata
author_sort Fernandes, Isabela Costa
collection PubMed
description Noncoding microRNAs are involved in lipid and carbohydrate metabolism pathways and are powerful regulators of gene expression. The goals of this study were to evaluate the temporal expression profiles of miRNAs in rat adipose tissue and predict mRNA–microRNA interactions. Newly weaned Wistar rats were divided into groups fed a standard diet and high-sucrose diet (HSD). The HSD contains 66.86% carbohydrates (40.45% standard diet, 40.45% condensed milk, and 8.58% crystal sugar), and the HSD was provided for 4, 8 and 15-week periods to investigate the expression levels of miRNAs in visceral adipose tissue using RT–qPCR. Target selection, enriched pathways and networks were analyzed in silico. The factor consumption time significantly was associated to decreases (p < 0.05) in the expression levels of the following miRNAs: 124-5p, 125-5p, 126-5p, 200c-3p, and 212-3p in all experimental groups. The factor diet significantly influenced rno-miR-124-5p, 200c-3p, and 212-3p expression (p < 0.05). A significant reduction (p < 0.05) in rno-miR-27a-3p expression was observed. The biological processes involved key pathways regulating fat deposition. Our findings provide important insights into downregulated miRNA expression patterns in visceral adipose tissue, adiposity level, hyperinsulinemia and increased VLDL-c and triglyceride levels.
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spelling pubmed-94600502022-09-10 Effects of the Long-Term Consumption of a High-Sucrose Diet on microRNA Expression in Visceral Adipose Tissue of Rats Fernandes, Isabela Costa Santos, Talita Adriana Pereira Oliveira, Daiane Teixeira Oliveira, Victor Fernandes Sousa, Graziele Galdino Pereira, Luciene Santos Barboza, Natália Rocha Evangelista, Elísio Alberto Guerra-Sá, Renata Nutrients Article Noncoding microRNAs are involved in lipid and carbohydrate metabolism pathways and are powerful regulators of gene expression. The goals of this study were to evaluate the temporal expression profiles of miRNAs in rat adipose tissue and predict mRNA–microRNA interactions. Newly weaned Wistar rats were divided into groups fed a standard diet and high-sucrose diet (HSD). The HSD contains 66.86% carbohydrates (40.45% standard diet, 40.45% condensed milk, and 8.58% crystal sugar), and the HSD was provided for 4, 8 and 15-week periods to investigate the expression levels of miRNAs in visceral adipose tissue using RT–qPCR. Target selection, enriched pathways and networks were analyzed in silico. The factor consumption time significantly was associated to decreases (p < 0.05) in the expression levels of the following miRNAs: 124-5p, 125-5p, 126-5p, 200c-3p, and 212-3p in all experimental groups. The factor diet significantly influenced rno-miR-124-5p, 200c-3p, and 212-3p expression (p < 0.05). A significant reduction (p < 0.05) in rno-miR-27a-3p expression was observed. The biological processes involved key pathways regulating fat deposition. Our findings provide important insights into downregulated miRNA expression patterns in visceral adipose tissue, adiposity level, hyperinsulinemia and increased VLDL-c and triglyceride levels. MDPI 2022-08-24 /pmc/articles/PMC9460050/ /pubmed/36079722 http://dx.doi.org/10.3390/nu14173465 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fernandes, Isabela Costa
Santos, Talita Adriana Pereira
Oliveira, Daiane Teixeira
Oliveira, Victor Fernandes
Sousa, Graziele Galdino
Pereira, Luciene Santos
Barboza, Natália Rocha
Evangelista, Elísio Alberto
Guerra-Sá, Renata
Effects of the Long-Term Consumption of a High-Sucrose Diet on microRNA Expression in Visceral Adipose Tissue of Rats
title Effects of the Long-Term Consumption of a High-Sucrose Diet on microRNA Expression in Visceral Adipose Tissue of Rats
title_full Effects of the Long-Term Consumption of a High-Sucrose Diet on microRNA Expression in Visceral Adipose Tissue of Rats
title_fullStr Effects of the Long-Term Consumption of a High-Sucrose Diet on microRNA Expression in Visceral Adipose Tissue of Rats
title_full_unstemmed Effects of the Long-Term Consumption of a High-Sucrose Diet on microRNA Expression in Visceral Adipose Tissue of Rats
title_short Effects of the Long-Term Consumption of a High-Sucrose Diet on microRNA Expression in Visceral Adipose Tissue of Rats
title_sort effects of the long-term consumption of a high-sucrose diet on microrna expression in visceral adipose tissue of rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9460050/
https://www.ncbi.nlm.nih.gov/pubmed/36079722
http://dx.doi.org/10.3390/nu14173465
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