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Bacterial Cellulose Membranes as Carriers for Nisin: Incorporation, Antimicrobial Activity, Cytotoxicity and Morphology

Based on the previous study, in which nisin and bacterial cellulose were utilized, this new experiment loads nisin into bacterial cellulose (N–BC) and evaluates the morphological characteristics, cytotoxicity, antimicrobial activity and stability of the developed system. The load efficiency of nisin...

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Autores principales: dos Santos, Gabriela Ribeiro, Soeiro, Victória Soares, Talarico, Carolina Fernanda, Ataide, Janaína Artem, Lopes, André Moreni, Mazzola, Priscila Gava, Oliveira, Thais Jardim, Oliveira Junior, José Martins, Grotto, Denise, Jozala, Angela F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9460746/
https://www.ncbi.nlm.nih.gov/pubmed/36080572
http://dx.doi.org/10.3390/polym14173497
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author dos Santos, Gabriela Ribeiro
Soeiro, Victória Soares
Talarico, Carolina Fernanda
Ataide, Janaína Artem
Lopes, André Moreni
Mazzola, Priscila Gava
Oliveira, Thais Jardim
Oliveira Junior, José Martins
Grotto, Denise
Jozala, Angela F.
author_facet dos Santos, Gabriela Ribeiro
Soeiro, Victória Soares
Talarico, Carolina Fernanda
Ataide, Janaína Artem
Lopes, André Moreni
Mazzola, Priscila Gava
Oliveira, Thais Jardim
Oliveira Junior, José Martins
Grotto, Denise
Jozala, Angela F.
author_sort dos Santos, Gabriela Ribeiro
collection PubMed
description Based on the previous study, in which nisin and bacterial cellulose were utilized, this new experiment loads nisin into bacterial cellulose (N–BC) and evaluates the morphological characteristics, cytotoxicity, antimicrobial activity and stability of the developed system. The load efficiency of nisin in BC was evaluated by an agar diffusion assay, utilizing Lactobacillus sakei, and total proteins. After having found the ideal time and concentration for the loading process, the system stability was evaluated for 100 days at 4, 25 and 37 °C against Staphylococcus aureus and L. sakei. Thus, in this study, there is a system that proves to be efficient, once BC has enhanced the antimicrobial activity of nisin, acting as a selective barrier for other compounds present in the standard solution and protecting the peptide. After 4 h, with 45% of proteins, this activity was almost 2 log(10) higher than that of the initial solution. Once the nisin solution was not pure, it is possible to suggest that the BC may have acted as a filter. This barrier enhanced the nisin activity and, as a consequence of the nisin loading, a stable N–BC system formed. The N–BC could create meaningful material for pharmaceutical and food applications.
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spelling pubmed-94607462022-09-10 Bacterial Cellulose Membranes as Carriers for Nisin: Incorporation, Antimicrobial Activity, Cytotoxicity and Morphology dos Santos, Gabriela Ribeiro Soeiro, Victória Soares Talarico, Carolina Fernanda Ataide, Janaína Artem Lopes, André Moreni Mazzola, Priscila Gava Oliveira, Thais Jardim Oliveira Junior, José Martins Grotto, Denise Jozala, Angela F. Polymers (Basel) Article Based on the previous study, in which nisin and bacterial cellulose were utilized, this new experiment loads nisin into bacterial cellulose (N–BC) and evaluates the morphological characteristics, cytotoxicity, antimicrobial activity and stability of the developed system. The load efficiency of nisin in BC was evaluated by an agar diffusion assay, utilizing Lactobacillus sakei, and total proteins. After having found the ideal time and concentration for the loading process, the system stability was evaluated for 100 days at 4, 25 and 37 °C against Staphylococcus aureus and L. sakei. Thus, in this study, there is a system that proves to be efficient, once BC has enhanced the antimicrobial activity of nisin, acting as a selective barrier for other compounds present in the standard solution and protecting the peptide. After 4 h, with 45% of proteins, this activity was almost 2 log(10) higher than that of the initial solution. Once the nisin solution was not pure, it is possible to suggest that the BC may have acted as a filter. This barrier enhanced the nisin activity and, as a consequence of the nisin loading, a stable N–BC system formed. The N–BC could create meaningful material for pharmaceutical and food applications. MDPI 2022-08-26 /pmc/articles/PMC9460746/ /pubmed/36080572 http://dx.doi.org/10.3390/polym14173497 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
dos Santos, Gabriela Ribeiro
Soeiro, Victória Soares
Talarico, Carolina Fernanda
Ataide, Janaína Artem
Lopes, André Moreni
Mazzola, Priscila Gava
Oliveira, Thais Jardim
Oliveira Junior, José Martins
Grotto, Denise
Jozala, Angela F.
Bacterial Cellulose Membranes as Carriers for Nisin: Incorporation, Antimicrobial Activity, Cytotoxicity and Morphology
title Bacterial Cellulose Membranes as Carriers for Nisin: Incorporation, Antimicrobial Activity, Cytotoxicity and Morphology
title_full Bacterial Cellulose Membranes as Carriers for Nisin: Incorporation, Antimicrobial Activity, Cytotoxicity and Morphology
title_fullStr Bacterial Cellulose Membranes as Carriers for Nisin: Incorporation, Antimicrobial Activity, Cytotoxicity and Morphology
title_full_unstemmed Bacterial Cellulose Membranes as Carriers for Nisin: Incorporation, Antimicrobial Activity, Cytotoxicity and Morphology
title_short Bacterial Cellulose Membranes as Carriers for Nisin: Incorporation, Antimicrobial Activity, Cytotoxicity and Morphology
title_sort bacterial cellulose membranes as carriers for nisin: incorporation, antimicrobial activity, cytotoxicity and morphology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9460746/
https://www.ncbi.nlm.nih.gov/pubmed/36080572
http://dx.doi.org/10.3390/polym14173497
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