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Genome-Wide Identification and Expression Analysis of Isopentenyl transferase Family Genes during Development and Resistance to Abiotic Stresses in Tea Plant (Camellia sinensis)

The tea plant is an important economic crop and is widely cultivated. Isopentenyl transferase (IPT) is the first and rate-limiting enzyme of cytokinin (CK) signaling, which plays key roles in plant development and abiotic stress. However, the IPT gene family in tea plants has not been systematically...

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Autores principales: Zhang, Liping, Li, Min, Fu, Jianyu, Huang, Xiaoqin, Yan, Peng, Ge, Shibei, Li, Zhengzhen, Bai, Peixian, Zhang, Lan, Han, Wenyan, Li, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9460862/
https://www.ncbi.nlm.nih.gov/pubmed/36079621
http://dx.doi.org/10.3390/plants11172243
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author Zhang, Liping
Li, Min
Fu, Jianyu
Huang, Xiaoqin
Yan, Peng
Ge, Shibei
Li, Zhengzhen
Bai, Peixian
Zhang, Lan
Han, Wenyan
Li, Xin
author_facet Zhang, Liping
Li, Min
Fu, Jianyu
Huang, Xiaoqin
Yan, Peng
Ge, Shibei
Li, Zhengzhen
Bai, Peixian
Zhang, Lan
Han, Wenyan
Li, Xin
author_sort Zhang, Liping
collection PubMed
description The tea plant is an important economic crop and is widely cultivated. Isopentenyl transferase (IPT) is the first and rate-limiting enzyme of cytokinin (CK) signaling, which plays key roles in plant development and abiotic stress. However, the IPT gene family in tea plants has not been systematically investigated until now. The phylogenetic analyses, gene structures, and conserved domains were predicted here. The results showed that a total of 13 CsIPT members were identified from a tea plant genome database and phylogenetically classified into four groups. Furthermore, 10 CsIPT members belonged to plant ADP/ATP-IPT genes, and 3 CsIPTs were tRNA-IPT genes. There is a conserved putative ATP/GTP-binding site (P-loop motif) in all the CsIPT sequences. Based on publicly available transcriptome data as well as through RNA-seq and qRT-PCR analysis, the CsIPT genes which play key roles in the development of different tissues were identified, respectively. Furthermore, CsIPT6.2 may be involved in the response to different light treatments. CsIPT6.4 may play a key role during the dormancy and flush of the lateral buds. CsIPT5.1 may play important regulatory roles during the development of the lateral bud, leaf, and flower. CsIPT5.2 and CsIPT6.2 may both play key roles for increased resistance to cold-stress, whereas CsIPT3.2 may play a key role in improving resistance to high-temperature stress as well as drought-stress and rewatering. This study could provide a reference for further studies of CsIPT family’s functions and could contribute to tea molecular breeding.
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spelling pubmed-94608622022-09-10 Genome-Wide Identification and Expression Analysis of Isopentenyl transferase Family Genes during Development and Resistance to Abiotic Stresses in Tea Plant (Camellia sinensis) Zhang, Liping Li, Min Fu, Jianyu Huang, Xiaoqin Yan, Peng Ge, Shibei Li, Zhengzhen Bai, Peixian Zhang, Lan Han, Wenyan Li, Xin Plants (Basel) Article The tea plant is an important economic crop and is widely cultivated. Isopentenyl transferase (IPT) is the first and rate-limiting enzyme of cytokinin (CK) signaling, which plays key roles in plant development and abiotic stress. However, the IPT gene family in tea plants has not been systematically investigated until now. The phylogenetic analyses, gene structures, and conserved domains were predicted here. The results showed that a total of 13 CsIPT members were identified from a tea plant genome database and phylogenetically classified into four groups. Furthermore, 10 CsIPT members belonged to plant ADP/ATP-IPT genes, and 3 CsIPTs were tRNA-IPT genes. There is a conserved putative ATP/GTP-binding site (P-loop motif) in all the CsIPT sequences. Based on publicly available transcriptome data as well as through RNA-seq and qRT-PCR analysis, the CsIPT genes which play key roles in the development of different tissues were identified, respectively. Furthermore, CsIPT6.2 may be involved in the response to different light treatments. CsIPT6.4 may play a key role during the dormancy and flush of the lateral buds. CsIPT5.1 may play important regulatory roles during the development of the lateral bud, leaf, and flower. CsIPT5.2 and CsIPT6.2 may both play key roles for increased resistance to cold-stress, whereas CsIPT3.2 may play a key role in improving resistance to high-temperature stress as well as drought-stress and rewatering. This study could provide a reference for further studies of CsIPT family’s functions and could contribute to tea molecular breeding. MDPI 2022-08-29 /pmc/articles/PMC9460862/ /pubmed/36079621 http://dx.doi.org/10.3390/plants11172243 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Liping
Li, Min
Fu, Jianyu
Huang, Xiaoqin
Yan, Peng
Ge, Shibei
Li, Zhengzhen
Bai, Peixian
Zhang, Lan
Han, Wenyan
Li, Xin
Genome-Wide Identification and Expression Analysis of Isopentenyl transferase Family Genes during Development and Resistance to Abiotic Stresses in Tea Plant (Camellia sinensis)
title Genome-Wide Identification and Expression Analysis of Isopentenyl transferase Family Genes during Development and Resistance to Abiotic Stresses in Tea Plant (Camellia sinensis)
title_full Genome-Wide Identification and Expression Analysis of Isopentenyl transferase Family Genes during Development and Resistance to Abiotic Stresses in Tea Plant (Camellia sinensis)
title_fullStr Genome-Wide Identification and Expression Analysis of Isopentenyl transferase Family Genes during Development and Resistance to Abiotic Stresses in Tea Plant (Camellia sinensis)
title_full_unstemmed Genome-Wide Identification and Expression Analysis of Isopentenyl transferase Family Genes during Development and Resistance to Abiotic Stresses in Tea Plant (Camellia sinensis)
title_short Genome-Wide Identification and Expression Analysis of Isopentenyl transferase Family Genes during Development and Resistance to Abiotic Stresses in Tea Plant (Camellia sinensis)
title_sort genome-wide identification and expression analysis of isopentenyl transferase family genes during development and resistance to abiotic stresses in tea plant (camellia sinensis)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9460862/
https://www.ncbi.nlm.nih.gov/pubmed/36079621
http://dx.doi.org/10.3390/plants11172243
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