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Optical bio-sensing of DNA methylation analysis: an overview of recent progress and future prospects

DNA methylation as one of the most important epigenetic modifications has a critical role in regulating gene expression and drug resistance in treating diseases such as cancer. Therefore, the detection of DNA methylation in the early stages of cancer plays an essential role in disease diagnosis. The...

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Autores principales: Adampourezare, Mina, Hasanzadeh, Mohammad, Seidi, Farzad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9460980/
https://www.ncbi.nlm.nih.gov/pubmed/36199327
http://dx.doi.org/10.1039/d2ra03630d
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author Adampourezare, Mina
Hasanzadeh, Mohammad
Seidi, Farzad
author_facet Adampourezare, Mina
Hasanzadeh, Mohammad
Seidi, Farzad
author_sort Adampourezare, Mina
collection PubMed
description DNA methylation as one of the most important epigenetic modifications has a critical role in regulating gene expression and drug resistance in treating diseases such as cancer. Therefore, the detection of DNA methylation in the early stages of cancer plays an essential role in disease diagnosis. The majority of routine methods to detect DNA methylation are very tedious and costly. Therefore, designing easy and sensitive methods to detect DNA methylation directly and without the need for molecular methods is a hot topic issue in bioscience. Here we provide an overview on the optical biosensors (including fluorescence, FRET, SERs, colorimetric) that have been applied to detect the DNA methylation. In addition, various types of labeled and label-free reactions along with the application of molecular methods and optical biosensors have been surveyed. Also, the effect of nanomaterials on the sensitivity of detection methods is discussed. Furthermore, a comprehensive overview of the advantages and disadvantages of each method are provided. Finally, the use of microfluidic devices in the evaluation of DNA methylation and DNA damage analysis based on smartphone detection has been discussed.
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spelling pubmed-94609802022-10-04 Optical bio-sensing of DNA methylation analysis: an overview of recent progress and future prospects Adampourezare, Mina Hasanzadeh, Mohammad Seidi, Farzad RSC Adv Chemistry DNA methylation as one of the most important epigenetic modifications has a critical role in regulating gene expression and drug resistance in treating diseases such as cancer. Therefore, the detection of DNA methylation in the early stages of cancer plays an essential role in disease diagnosis. The majority of routine methods to detect DNA methylation are very tedious and costly. Therefore, designing easy and sensitive methods to detect DNA methylation directly and without the need for molecular methods is a hot topic issue in bioscience. Here we provide an overview on the optical biosensors (including fluorescence, FRET, SERs, colorimetric) that have been applied to detect the DNA methylation. In addition, various types of labeled and label-free reactions along with the application of molecular methods and optical biosensors have been surveyed. Also, the effect of nanomaterials on the sensitivity of detection methods is discussed. Furthermore, a comprehensive overview of the advantages and disadvantages of each method are provided. Finally, the use of microfluidic devices in the evaluation of DNA methylation and DNA damage analysis based on smartphone detection has been discussed. The Royal Society of Chemistry 2022-09-09 /pmc/articles/PMC9460980/ /pubmed/36199327 http://dx.doi.org/10.1039/d2ra03630d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Adampourezare, Mina
Hasanzadeh, Mohammad
Seidi, Farzad
Optical bio-sensing of DNA methylation analysis: an overview of recent progress and future prospects
title Optical bio-sensing of DNA methylation analysis: an overview of recent progress and future prospects
title_full Optical bio-sensing of DNA methylation analysis: an overview of recent progress and future prospects
title_fullStr Optical bio-sensing of DNA methylation analysis: an overview of recent progress and future prospects
title_full_unstemmed Optical bio-sensing of DNA methylation analysis: an overview of recent progress and future prospects
title_short Optical bio-sensing of DNA methylation analysis: an overview of recent progress and future prospects
title_sort optical bio-sensing of dna methylation analysis: an overview of recent progress and future prospects
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9460980/
https://www.ncbi.nlm.nih.gov/pubmed/36199327
http://dx.doi.org/10.1039/d2ra03630d
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