Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022

Anal and urethral samples from confirmed cases of monkeypox were screened for monkeypox virus (MPXV) by real-time PCR. Isolation of the virus was subsequently attempted in cell culture. Actively-replicating virus was demonstrated in 13 of 18 and 11 of 15 PCR-positive anal and urethral swabs, respect...

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Detalles Bibliográficos
Autores principales: Moschese, Davide, Pozza, Giacomo, Mileto, Davide, Giacomelli, Andrea, Cutrera, Miriam, Cossu, Maria Vittoria, Matone, Maddalena, Beltrami, Martina, Salari, Federica, Antinori, Spinello, Lombardi, Alessandra, Rizzardini, Giuliano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2022
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9461308/
https://www.ncbi.nlm.nih.gov/pubmed/36082684
http://dx.doi.org/10.2807/1560-7917.ES.2022.27.36.2200675
Descripción
Sumario:Anal and urethral samples from confirmed cases of monkeypox were screened for monkeypox virus (MPXV) by real-time PCR. Isolation of the virus was subsequently attempted in cell culture. Actively-replicating virus was demonstrated in 13 of 18 and 11 of 15 PCR-positive anal and urethral swabs, respectively, collected within 7 days from symptoms onset. Two asymptomatic secondary cases had detectable MPXV genetic material in urethral secretion and for one, MPXV was successfully isolated, supporting a potential MPXV sexual transmission hypothesis.

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