Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022

Anal and urethral samples from confirmed cases of monkeypox were screened for monkeypox virus (MPXV) by real-time PCR. Isolation of the virus was subsequently attempted in cell culture. Actively-replicating virus was demonstrated in 13 of 18 and 11 of 15 PCR-positive anal and urethral swabs, respect...

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Autores principales: Moschese, Davide, Pozza, Giacomo, Mileto, Davide, Giacomelli, Andrea, Cutrera, Miriam, Cossu, Maria Vittoria, Matone, Maddalena, Beltrami, Martina, Salari, Federica, Antinori, Spinello, Lombardi, Alessandra, Rizzardini, Giuliano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2022
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9461308/
https://www.ncbi.nlm.nih.gov/pubmed/36082684
http://dx.doi.org/10.2807/1560-7917.ES.2022.27.36.2200675
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author Moschese, Davide
Pozza, Giacomo
Mileto, Davide
Giacomelli, Andrea
Cutrera, Miriam
Cossu, Maria Vittoria
Matone, Maddalena
Beltrami, Martina
Salari, Federica
Antinori, Spinello
Lombardi, Alessandra
Rizzardini, Giuliano
author_facet Moschese, Davide
Pozza, Giacomo
Mileto, Davide
Giacomelli, Andrea
Cutrera, Miriam
Cossu, Maria Vittoria
Matone, Maddalena
Beltrami, Martina
Salari, Federica
Antinori, Spinello
Lombardi, Alessandra
Rizzardini, Giuliano
author_sort Moschese, Davide
collection PubMed
description Anal and urethral samples from confirmed cases of monkeypox were screened for monkeypox virus (MPXV) by real-time PCR. Isolation of the virus was subsequently attempted in cell culture. Actively-replicating virus was demonstrated in 13 of 18 and 11 of 15 PCR-positive anal and urethral swabs, respectively, collected within 7 days from symptoms onset. Two asymptomatic secondary cases had detectable MPXV genetic material in urethral secretion and for one, MPXV was successfully isolated, supporting a potential MPXV sexual transmission hypothesis.
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spelling pubmed-94613082022-09-23 Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022 Moschese, Davide Pozza, Giacomo Mileto, Davide Giacomelli, Andrea Cutrera, Miriam Cossu, Maria Vittoria Matone, Maddalena Beltrami, Martina Salari, Federica Antinori, Spinello Lombardi, Alessandra Rizzardini, Giuliano Euro Surveill Rapid Communication Anal and urethral samples from confirmed cases of monkeypox were screened for monkeypox virus (MPXV) by real-time PCR. Isolation of the virus was subsequently attempted in cell culture. Actively-replicating virus was demonstrated in 13 of 18 and 11 of 15 PCR-positive anal and urethral swabs, respectively, collected within 7 days from symptoms onset. Two asymptomatic secondary cases had detectable MPXV genetic material in urethral secretion and for one, MPXV was successfully isolated, supporting a potential MPXV sexual transmission hypothesis. European Centre for Disease Prevention and Control (ECDC) 2022-09-08 /pmc/articles/PMC9461308/ /pubmed/36082684 http://dx.doi.org/10.2807/1560-7917.ES.2022.27.36.2200675 Text en This article is copyright of the authors or their affiliated institutions, 2022. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.
spellingShingle Rapid Communication
Moschese, Davide
Pozza, Giacomo
Mileto, Davide
Giacomelli, Andrea
Cutrera, Miriam
Cossu, Maria Vittoria
Matone, Maddalena
Beltrami, Martina
Salari, Federica
Antinori, Spinello
Lombardi, Alessandra
Rizzardini, Giuliano
Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022
title Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022
title_full Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022
title_fullStr Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022
title_full_unstemmed Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022
title_short Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022
title_sort isolation of viable monkeypox virus from anal and urethral swabs, italy, may to july 2022
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9461308/
https://www.ncbi.nlm.nih.gov/pubmed/36082684
http://dx.doi.org/10.2807/1560-7917.ES.2022.27.36.2200675
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