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Alterations of gut microbial pathways and virulence factors in hemodialysis patients

Alterations in gut microbiota might contribute to uremic toxicity and immune dysregulation in patients with end-stage renal disease. Hemodialysis patients are prone to infection and higher mortality following sepsis. The virulence factors in the gut metagenome have not been well studied in hemodialy...

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Autores principales: Shi, Xiaochun, Gao, Bei, Srivastava, Anvesha, Izzi, Zahra, Abdalla, Yoosif, Shen, Weishou, Raj, Dominic
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9461950/
https://www.ncbi.nlm.nih.gov/pubmed/36093194
http://dx.doi.org/10.3389/fcimb.2022.904284
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author Shi, Xiaochun
Gao, Bei
Srivastava, Anvesha
Izzi, Zahra
Abdalla, Yoosif
Shen, Weishou
Raj, Dominic
author_facet Shi, Xiaochun
Gao, Bei
Srivastava, Anvesha
Izzi, Zahra
Abdalla, Yoosif
Shen, Weishou
Raj, Dominic
author_sort Shi, Xiaochun
collection PubMed
description Alterations in gut microbiota might contribute to uremic toxicity and immune dysregulation in patients with end-stage renal disease. Hemodialysis patients are prone to infection and higher mortality following sepsis. The virulence factors in the gut metagenome have not been well studied in hemodialysis patients, which could be employed by microorganisms to successfully thrive and flourish in their hosts. In this study, we performed shotgun metagenomics sequencing on fecal DNA collected from 16 control subjects and 24 hemodialysis patients. Our analysis shows that a number of microbial species, metabolic pathways, antibiotic resistance, and virulence factors were significantly altered in hemodialysis patients compared with controls. In particular, erythromycin resistance methylase, pyridoxamine 5-phosphate oxidase, and streptothricin-acetyl-transferase were significantly increased in hemodialysis patients. The findings in our study laid a valuable foundation to further elucidate the causative role of virulence factors in predisposing HD patients to infection and to develop treatment strategies to reduce the genetic capacities of antibiotic resistance and virulence factors in HD patients.
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spelling pubmed-94619502022-09-10 Alterations of gut microbial pathways and virulence factors in hemodialysis patients Shi, Xiaochun Gao, Bei Srivastava, Anvesha Izzi, Zahra Abdalla, Yoosif Shen, Weishou Raj, Dominic Front Cell Infect Microbiol Cellular and Infection Microbiology Alterations in gut microbiota might contribute to uremic toxicity and immune dysregulation in patients with end-stage renal disease. Hemodialysis patients are prone to infection and higher mortality following sepsis. The virulence factors in the gut metagenome have not been well studied in hemodialysis patients, which could be employed by microorganisms to successfully thrive and flourish in their hosts. In this study, we performed shotgun metagenomics sequencing on fecal DNA collected from 16 control subjects and 24 hemodialysis patients. Our analysis shows that a number of microbial species, metabolic pathways, antibiotic resistance, and virulence factors were significantly altered in hemodialysis patients compared with controls. In particular, erythromycin resistance methylase, pyridoxamine 5-phosphate oxidase, and streptothricin-acetyl-transferase were significantly increased in hemodialysis patients. The findings in our study laid a valuable foundation to further elucidate the causative role of virulence factors in predisposing HD patients to infection and to develop treatment strategies to reduce the genetic capacities of antibiotic resistance and virulence factors in HD patients. Frontiers Media S.A. 2022-08-26 /pmc/articles/PMC9461950/ /pubmed/36093194 http://dx.doi.org/10.3389/fcimb.2022.904284 Text en Copyright © 2022 Shi, Gao, Srivastava, Izzi, Abdalla, Shen and Raj https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Shi, Xiaochun
Gao, Bei
Srivastava, Anvesha
Izzi, Zahra
Abdalla, Yoosif
Shen, Weishou
Raj, Dominic
Alterations of gut microbial pathways and virulence factors in hemodialysis patients
title Alterations of gut microbial pathways and virulence factors in hemodialysis patients
title_full Alterations of gut microbial pathways and virulence factors in hemodialysis patients
title_fullStr Alterations of gut microbial pathways and virulence factors in hemodialysis patients
title_full_unstemmed Alterations of gut microbial pathways and virulence factors in hemodialysis patients
title_short Alterations of gut microbial pathways and virulence factors in hemodialysis patients
title_sort alterations of gut microbial pathways and virulence factors in hemodialysis patients
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9461950/
https://www.ncbi.nlm.nih.gov/pubmed/36093194
http://dx.doi.org/10.3389/fcimb.2022.904284
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