Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants

We designed a protein biosensor that uses thermodynamic coupling for sensitive and rapid detection of neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in serum. The biosensor is a switchable, caged luciferase–receptor-binding domain (RBD) construc...

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Detalles Bibliográficos
Autores principales: Zhang, Jason Z., Yeh, Hsien-Wei, Walls, Alexandra C., Wicky, Basile I. M., Sprouse, Kaitlin R., VanBlargan, Laura A., Treger, Rebecca, Quijano-Rubio, Alfredo, Pham, Minh N., Kraft, John C., Haydon, Ian C., Yang, Wei, DeWitt, Michelle, Bowen, John E., Chow, Cameron M., Carter, Lauren, Ravichandran, Rashmi, Wener, Mark H., Stewart, Lance, Veesler, David, Diamond, Michael S., Greninger, Alexander L., Koelle, David M., Baker, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9463068/
https://www.ncbi.nlm.nih.gov/pubmed/35484405
http://dx.doi.org/10.1038/s41587-022-01280-8
Descripción
Sumario:We designed a protein biosensor that uses thermodynamic coupling for sensitive and rapid detection of neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in serum. The biosensor is a switchable, caged luciferase–receptor-binding domain (RBD) construct that detects serum-antibody interference with the binding of virus RBD to angiotensin-converting enzyme 2 (ACE-2) as a proxy for neutralization. Our coupling approach does not require target modification and can better distinguish sample-to-sample differences in analyte binding affinity and abundance than traditional competition-based assays.

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