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Role of magnesium-doped calcium sulfate and β-tricalcium phosphate composite ceramics in macrophage polarization and osteo-induction
In the current study, we explored the role of Mg(2+)-doped CaSO(4)/β-TCP composite biopolymer in regulating macrophage polarization and its relation with enhanced osteogenic differentiation of periodontal ligament stem cells. Furthermore, mechanism underling the regulation of macrophage polarization...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Nature Singapore
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9463206/ https://www.ncbi.nlm.nih.gov/pubmed/35653001 http://dx.doi.org/10.1007/s10266-022-00708-6 |
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author | Zhou, Jing Sun, Su He, Yan Yan, Tingting Sun, Jianfeng Pan, Jie Zhu, Shuyu Chen, Liqiong Zhu, Pengfei Xu, Biao Liu, Yan |
author_facet | Zhou, Jing Sun, Su He, Yan Yan, Tingting Sun, Jianfeng Pan, Jie Zhu, Shuyu Chen, Liqiong Zhu, Pengfei Xu, Biao Liu, Yan |
author_sort | Zhou, Jing |
collection | PubMed |
description | In the current study, we explored the role of Mg(2+)-doped CaSO(4)/β-TCP composite biopolymer in regulating macrophage polarization and its relation with enhanced osteogenic differentiation of periodontal ligament stem cells. Furthermore, mechanism underling the regulation of macrophage polarization by CaSO(4)/β-TCP was evaluated. Mg(2+)-doped CaSO(4)/β-TCP composite was characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD). Macrophage polarization was characterized using flow cytometry analysis. Macrophage morphometric analysis was conducted by FITC phalloidin staining. Western blot and qRT-PCR assays were used to assess gene expression levels and miRNAs, respectively. SEM morphology of CaSO(4)/β-TCP ceramic revealed a particle size of 10–50 μm, and XRD spectrum showed that characteristic peak of samples was consistent with that of CaSO(4) and β-TCP. Results from flow cytometry evidenced significant upregulation of M2 macrophage markers after adding ceramic biopolymer, indicating the induction of inactivated M0 macrophage polarization to M2 macrophage. Macrophage morphometric analysis revealed development of lamellar pseudopodia on day 7 in CaSO(4)/β-TCP group. Furthermore, flow cytometry revealed high positivity rate of 90.34% (CD44) and 89.36% (CD146). qRT-PCR results showed that the level of miR-21-5p was significantly decreased in M2 macrophages. Moreover, western blot analysis revealed upregulated expression levels of RUNX2, osterix (Osx), and osteopontin (OPN), and ELISA exhibited increase in cytokine levels (IL-1β, IL-10, TGF-β1, and BMP-2) in the presence of macrophages, indicating the osteogenic differentiation ability of periodontal ligament stem cells. The study evidenced the regulation of macrophage polarization by Mg(2+)-doped CaSO(4)/β-TCP composite ceramic and its mediation through lncRNA PVT1/miR-21-5p/smad2 molecular axis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10266-022-00708-6. |
format | Online Article Text |
id | pubmed-9463206 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Nature Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-94632062022-09-11 Role of magnesium-doped calcium sulfate and β-tricalcium phosphate composite ceramics in macrophage polarization and osteo-induction Zhou, Jing Sun, Su He, Yan Yan, Tingting Sun, Jianfeng Pan, Jie Zhu, Shuyu Chen, Liqiong Zhu, Pengfei Xu, Biao Liu, Yan Odontology Original Article In the current study, we explored the role of Mg(2+)-doped CaSO(4)/β-TCP composite biopolymer in regulating macrophage polarization and its relation with enhanced osteogenic differentiation of periodontal ligament stem cells. Furthermore, mechanism underling the regulation of macrophage polarization by CaSO(4)/β-TCP was evaluated. Mg(2+)-doped CaSO(4)/β-TCP composite was characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD). Macrophage polarization was characterized using flow cytometry analysis. Macrophage morphometric analysis was conducted by FITC phalloidin staining. Western blot and qRT-PCR assays were used to assess gene expression levels and miRNAs, respectively. SEM morphology of CaSO(4)/β-TCP ceramic revealed a particle size of 10–50 μm, and XRD spectrum showed that characteristic peak of samples was consistent with that of CaSO(4) and β-TCP. Results from flow cytometry evidenced significant upregulation of M2 macrophage markers after adding ceramic biopolymer, indicating the induction of inactivated M0 macrophage polarization to M2 macrophage. Macrophage morphometric analysis revealed development of lamellar pseudopodia on day 7 in CaSO(4)/β-TCP group. Furthermore, flow cytometry revealed high positivity rate of 90.34% (CD44) and 89.36% (CD146). qRT-PCR results showed that the level of miR-21-5p was significantly decreased in M2 macrophages. Moreover, western blot analysis revealed upregulated expression levels of RUNX2, osterix (Osx), and osteopontin (OPN), and ELISA exhibited increase in cytokine levels (IL-1β, IL-10, TGF-β1, and BMP-2) in the presence of macrophages, indicating the osteogenic differentiation ability of periodontal ligament stem cells. The study evidenced the regulation of macrophage polarization by Mg(2+)-doped CaSO(4)/β-TCP composite ceramic and its mediation through lncRNA PVT1/miR-21-5p/smad2 molecular axis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10266-022-00708-6. Springer Nature Singapore 2022-06-02 2022 /pmc/articles/PMC9463206/ /pubmed/35653001 http://dx.doi.org/10.1007/s10266-022-00708-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Zhou, Jing Sun, Su He, Yan Yan, Tingting Sun, Jianfeng Pan, Jie Zhu, Shuyu Chen, Liqiong Zhu, Pengfei Xu, Biao Liu, Yan Role of magnesium-doped calcium sulfate and β-tricalcium phosphate composite ceramics in macrophage polarization and osteo-induction |
title | Role of magnesium-doped calcium sulfate and β-tricalcium phosphate composite ceramics in macrophage polarization and osteo-induction |
title_full | Role of magnesium-doped calcium sulfate and β-tricalcium phosphate composite ceramics in macrophage polarization and osteo-induction |
title_fullStr | Role of magnesium-doped calcium sulfate and β-tricalcium phosphate composite ceramics in macrophage polarization and osteo-induction |
title_full_unstemmed | Role of magnesium-doped calcium sulfate and β-tricalcium phosphate composite ceramics in macrophage polarization and osteo-induction |
title_short | Role of magnesium-doped calcium sulfate and β-tricalcium phosphate composite ceramics in macrophage polarization and osteo-induction |
title_sort | role of magnesium-doped calcium sulfate and β-tricalcium phosphate composite ceramics in macrophage polarization and osteo-induction |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9463206/ https://www.ncbi.nlm.nih.gov/pubmed/35653001 http://dx.doi.org/10.1007/s10266-022-00708-6 |
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