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α-Enolase inhibits apoptosis and promotes cell invasion and proliferation of skin cutaneous melanoma
BACKGROUND: The glycolytic enzyme, α-Enolase (ENO1), catalyzes the production of phosphoenolpyruvate from 2-phosphoglycerate, thereby enhancing glycolysis and contributing to tumor progression. In the present study, we aimed to determine the role of ENO1 in skin cutaneous melanoma (SKCM) and the pot...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9463226/ https://www.ncbi.nlm.nih.gov/pubmed/35925486 http://dx.doi.org/10.1007/s11033-022-07540-9 |
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author | Zhang, Kun Tian, Ruoxi Zhang, Wancong Li, Yishuai Zeng, Ning Liang, Yan Tang, Shijie |
author_facet | Zhang, Kun Tian, Ruoxi Zhang, Wancong Li, Yishuai Zeng, Ning Liang, Yan Tang, Shijie |
author_sort | Zhang, Kun |
collection | PubMed |
description | BACKGROUND: The glycolytic enzyme, α-Enolase (ENO1), catalyzes the production of phosphoenolpyruvate from 2-phosphoglycerate, thereby enhancing glycolysis and contributing to tumor progression. In the present study, we aimed to determine the role of ENO1 in skin cutaneous melanoma (SKCM) and the potential underlying mechanism. METHODS: The Sangerbox database was used to analyze the mRNA expression of ENO1 in SKCM. Western blotting was used to assess the levels of ENO1, c-Myc, β-catenin, MMP-9, PGAM1, and MMP-13 in SKCM-derived cell lines or tumor tissues from patients with SKCM. The pCMV-SPORT6-ENO1 and pET-28a-ENO1siRNA plasmids were used to overexpress and knockdown ENO1 in SKCM cells, respectively. To determine the function of ENO1 in the malignant behavior of SKCM cells, we performed a wound-healing assay, cell counting kit 8 assay, and transwell chamber analyses. The production of pyruvate and lactic acid in tumor cells was evaluated using their respective kits. RESULTS: Compared with non-tumor tissues, ENO1 was found to be overexpressed in SKCM tissues. In SKCM cells, ENO1 overexpression promoted invasion, migration, and proliferation of tumor cells; increased pyruvate and lactate production; and increased β-catenin, MMP-9, MMP-13, and c-Myc levels. The opposite effects were observed in SKCM cells silenced for ENO1. CONCLUSIONS: These results indicate that ENO1 is involved in SKCM progression by enhancing the invasion and proliferation of tumor cells. In addition, ENO1 might have an important function in tumor cell glycolysis. Therefore, ENO1 represents a potential therapeutic target for treatment of SKCM. |
format | Online Article Text |
id | pubmed-9463226 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-94632262022-09-11 α-Enolase inhibits apoptosis and promotes cell invasion and proliferation of skin cutaneous melanoma Zhang, Kun Tian, Ruoxi Zhang, Wancong Li, Yishuai Zeng, Ning Liang, Yan Tang, Shijie Mol Biol Rep Original Article BACKGROUND: The glycolytic enzyme, α-Enolase (ENO1), catalyzes the production of phosphoenolpyruvate from 2-phosphoglycerate, thereby enhancing glycolysis and contributing to tumor progression. In the present study, we aimed to determine the role of ENO1 in skin cutaneous melanoma (SKCM) and the potential underlying mechanism. METHODS: The Sangerbox database was used to analyze the mRNA expression of ENO1 in SKCM. Western blotting was used to assess the levels of ENO1, c-Myc, β-catenin, MMP-9, PGAM1, and MMP-13 in SKCM-derived cell lines or tumor tissues from patients with SKCM. The pCMV-SPORT6-ENO1 and pET-28a-ENO1siRNA plasmids were used to overexpress and knockdown ENO1 in SKCM cells, respectively. To determine the function of ENO1 in the malignant behavior of SKCM cells, we performed a wound-healing assay, cell counting kit 8 assay, and transwell chamber analyses. The production of pyruvate and lactic acid in tumor cells was evaluated using their respective kits. RESULTS: Compared with non-tumor tissues, ENO1 was found to be overexpressed in SKCM tissues. In SKCM cells, ENO1 overexpression promoted invasion, migration, and proliferation of tumor cells; increased pyruvate and lactate production; and increased β-catenin, MMP-9, MMP-13, and c-Myc levels. The opposite effects were observed in SKCM cells silenced for ENO1. CONCLUSIONS: These results indicate that ENO1 is involved in SKCM progression by enhancing the invasion and proliferation of tumor cells. In addition, ENO1 might have an important function in tumor cell glycolysis. Therefore, ENO1 represents a potential therapeutic target for treatment of SKCM. Springer Netherlands 2022-08-04 2022 /pmc/articles/PMC9463226/ /pubmed/35925486 http://dx.doi.org/10.1007/s11033-022-07540-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Zhang, Kun Tian, Ruoxi Zhang, Wancong Li, Yishuai Zeng, Ning Liang, Yan Tang, Shijie α-Enolase inhibits apoptosis and promotes cell invasion and proliferation of skin cutaneous melanoma |
title | α-Enolase inhibits apoptosis and promotes cell invasion and proliferation of skin cutaneous melanoma |
title_full | α-Enolase inhibits apoptosis and promotes cell invasion and proliferation of skin cutaneous melanoma |
title_fullStr | α-Enolase inhibits apoptosis and promotes cell invasion and proliferation of skin cutaneous melanoma |
title_full_unstemmed | α-Enolase inhibits apoptosis and promotes cell invasion and proliferation of skin cutaneous melanoma |
title_short | α-Enolase inhibits apoptosis and promotes cell invasion and proliferation of skin cutaneous melanoma |
title_sort | α-enolase inhibits apoptosis and promotes cell invasion and proliferation of skin cutaneous melanoma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9463226/ https://www.ncbi.nlm.nih.gov/pubmed/35925486 http://dx.doi.org/10.1007/s11033-022-07540-9 |
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