Protocol for affordable and efficient profiling of nascent RNAs in bread wheat using GRO-seq

Exorbitant sequencing cost is one of the main obstacles limiting the widespread application of Global Run-On sequencing (GRO-seq) to detect transcriptional activity. Here, we describe a more efficient and affordable protocol for GRO-seq that incorporates an rRNA removal step after nuclear RNA isolat...

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Detalles Bibliográficos
Autores principales: Chen, Yan, Zhu, Jiafu, Xie, Yilin, Li, Zijuan, Zhang, Yijing, Liu, Min, Dong, Zhicheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9463444/
https://www.ncbi.nlm.nih.gov/pubmed/36097381
http://dx.doi.org/10.1016/j.xpro.2022.101657

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