Cargando…

Microporous structures on mineralized collagen mediate osteogenesis by modulating the osteo-immune response of macrophages

It is a new hot pot in tissue engineering and regenerative medicine to study the effects of physicochemical properties of implanted biomaterials on regulating macrophage polarization to promote bone regeneration. In this study, we designed and fabricated mineralized collagen (MC) with different micr...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Jun, Luo, Xin, Lv, Zhao-Yong, Qiang, Hui-Fen, Hou, Cai-Yao, Liu, Kun, Meng, Chun-Xiu, Zhang, Yu-Jue, Liu, Feng-Zhen, Zhang, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9464819/
https://www.ncbi.nlm.nih.gov/pubmed/36105601
http://dx.doi.org/10.3389/fbioe.2022.917655
_version_ 1784787653582913536
author Li, Jun
Luo, Xin
Lv, Zhao-Yong
Qiang, Hui-Fen
Hou, Cai-Yao
Liu, Kun
Meng, Chun-Xiu
Zhang, Yu-Jue
Liu, Feng-Zhen
Zhang, Bin
author_facet Li, Jun
Luo, Xin
Lv, Zhao-Yong
Qiang, Hui-Fen
Hou, Cai-Yao
Liu, Kun
Meng, Chun-Xiu
Zhang, Yu-Jue
Liu, Feng-Zhen
Zhang, Bin
author_sort Li, Jun
collection PubMed
description It is a new hot pot in tissue engineering and regenerative medicine to study the effects of physicochemical properties of implanted biomaterials on regulating macrophage polarization to promote bone regeneration. In this study, we designed and fabricated mineralized collagen (MC) with different microporous structures via in vitro biomimetic mineralization method. The microporous structures, mechanical properties, shore hardness and water contact angle measurements were tested. Live/dead cell staining, CCK-8 assay, phalloidine staining, staining of focal adhesions were used to detect cell behavior. ELISA, qRT-PCR, ALP, and alizarin red staining (ARS) were performed to appraise osteogenic differentiation and investigated macrophage response and their subsequent effects on the osteogenic differentiation. The results showed that RAW264.7 and MC3T3-E1 cells were able to survive on the MC. MC with the microporous structure of approximately 84 μm and 70%–80% porosity could promote M2 macrophage polarization and increase the expression level of TGF-β and VEGF. Moreover, the gene expression of the osteogenic markers ALP, COL-1, and OCN increased. Therefore, MC with different microporous structures mediated osteoimmunomodulation in bone regeneration. These data will provide a new idea of biomaterials inducing bone repair and direct the optimal design of novel immune biomaterials, development, and rational usage.
format Online
Article
Text
id pubmed-9464819
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-94648192022-09-13 Microporous structures on mineralized collagen mediate osteogenesis by modulating the osteo-immune response of macrophages Li, Jun Luo, Xin Lv, Zhao-Yong Qiang, Hui-Fen Hou, Cai-Yao Liu, Kun Meng, Chun-Xiu Zhang, Yu-Jue Liu, Feng-Zhen Zhang, Bin Front Bioeng Biotechnol Bioengineering and Biotechnology It is a new hot pot in tissue engineering and regenerative medicine to study the effects of physicochemical properties of implanted biomaterials on regulating macrophage polarization to promote bone regeneration. In this study, we designed and fabricated mineralized collagen (MC) with different microporous structures via in vitro biomimetic mineralization method. The microporous structures, mechanical properties, shore hardness and water contact angle measurements were tested. Live/dead cell staining, CCK-8 assay, phalloidine staining, staining of focal adhesions were used to detect cell behavior. ELISA, qRT-PCR, ALP, and alizarin red staining (ARS) were performed to appraise osteogenic differentiation and investigated macrophage response and their subsequent effects on the osteogenic differentiation. The results showed that RAW264.7 and MC3T3-E1 cells were able to survive on the MC. MC with the microporous structure of approximately 84 μm and 70%–80% porosity could promote M2 macrophage polarization and increase the expression level of TGF-β and VEGF. Moreover, the gene expression of the osteogenic markers ALP, COL-1, and OCN increased. Therefore, MC with different microporous structures mediated osteoimmunomodulation in bone regeneration. These data will provide a new idea of biomaterials inducing bone repair and direct the optimal design of novel immune biomaterials, development, and rational usage. Frontiers Media S.A. 2022-08-29 /pmc/articles/PMC9464819/ /pubmed/36105601 http://dx.doi.org/10.3389/fbioe.2022.917655 Text en Copyright © 2022 Li, Luo, Lv, Qiang, Hou, Liu, Meng, Zhang, Liu and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Li, Jun
Luo, Xin
Lv, Zhao-Yong
Qiang, Hui-Fen
Hou, Cai-Yao
Liu, Kun
Meng, Chun-Xiu
Zhang, Yu-Jue
Liu, Feng-Zhen
Zhang, Bin
Microporous structures on mineralized collagen mediate osteogenesis by modulating the osteo-immune response of macrophages
title Microporous structures on mineralized collagen mediate osteogenesis by modulating the osteo-immune response of macrophages
title_full Microporous structures on mineralized collagen mediate osteogenesis by modulating the osteo-immune response of macrophages
title_fullStr Microporous structures on mineralized collagen mediate osteogenesis by modulating the osteo-immune response of macrophages
title_full_unstemmed Microporous structures on mineralized collagen mediate osteogenesis by modulating the osteo-immune response of macrophages
title_short Microporous structures on mineralized collagen mediate osteogenesis by modulating the osteo-immune response of macrophages
title_sort microporous structures on mineralized collagen mediate osteogenesis by modulating the osteo-immune response of macrophages
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9464819/
https://www.ncbi.nlm.nih.gov/pubmed/36105601
http://dx.doi.org/10.3389/fbioe.2022.917655
work_keys_str_mv AT lijun microporousstructuresonmineralizedcollagenmediateosteogenesisbymodulatingtheosteoimmuneresponseofmacrophages
AT luoxin microporousstructuresonmineralizedcollagenmediateosteogenesisbymodulatingtheosteoimmuneresponseofmacrophages
AT lvzhaoyong microporousstructuresonmineralizedcollagenmediateosteogenesisbymodulatingtheosteoimmuneresponseofmacrophages
AT qianghuifen microporousstructuresonmineralizedcollagenmediateosteogenesisbymodulatingtheosteoimmuneresponseofmacrophages
AT houcaiyao microporousstructuresonmineralizedcollagenmediateosteogenesisbymodulatingtheosteoimmuneresponseofmacrophages
AT liukun microporousstructuresonmineralizedcollagenmediateosteogenesisbymodulatingtheosteoimmuneresponseofmacrophages
AT mengchunxiu microporousstructuresonmineralizedcollagenmediateosteogenesisbymodulatingtheosteoimmuneresponseofmacrophages
AT zhangyujue microporousstructuresonmineralizedcollagenmediateosteogenesisbymodulatingtheosteoimmuneresponseofmacrophages
AT liufengzhen microporousstructuresonmineralizedcollagenmediateosteogenesisbymodulatingtheosteoimmuneresponseofmacrophages
AT zhangbin microporousstructuresonmineralizedcollagenmediateosteogenesisbymodulatingtheosteoimmuneresponseofmacrophages