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Plasma vesicular miR-155 as a biomarker of immune activation in antiretroviral treated people living with HIV
People living with HIV (PLWH), despite suppression of viral replication with antiretroviral therapy (ART), have high morbidity and mortality due to immune activation and chronic inflammation. Discovering new biomarkers of immune activation status under ART will be pertinent to improve PLWH quality o...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9464867/ https://www.ncbi.nlm.nih.gov/pubmed/36105810 http://dx.doi.org/10.3389/fimmu.2022.916599 |
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author | Bazié, Wilfried Wenceslas Boucher, Julien Goyer, Benjamin Traoré, Isidore Tiandiogo Kania, Dramane Somé, Diane Yirgnur Alary, Michel Gilbert, Caroline |
author_facet | Bazié, Wilfried Wenceslas Boucher, Julien Goyer, Benjamin Traoré, Isidore Tiandiogo Kania, Dramane Somé, Diane Yirgnur Alary, Michel Gilbert, Caroline |
author_sort | Bazié, Wilfried Wenceslas |
collection | PubMed |
description | People living with HIV (PLWH), despite suppression of viral replication with antiretroviral therapy (ART), have high morbidity and mortality due to immune activation and chronic inflammation. Discovering new biomarkers of immune activation status under ART will be pertinent to improve PLWH quality of life when the majority will be treated. We stipulate that plasma large and small extracellular vesicle (EVs) and their microRNA content could be easily measured biomarkers to monitor immune activation in PLWH. Venous blood samples from n = 128 ART-treated PLWH with suppressed viral load (≤ 20 copies/mL) and n = 60 HIV-uninfected participants were collected at five testing or treatment centers of PLWH in Burkina Faso. Large and small plasma EVs were purified, counted, and the mature miRNAs miR-29a, miR-146a, and miR-155 were quantified by RT-qPCR. Diagnostic performances of large and small EVs miRNAs level were evaluated by receiver operating characteristic (ROC) curve analysis and principal component analysis (PCA). Among the EVs microRNA measured, only large EVs miR-155 copies distinguished PLWH with immune activation, with AUC of 0.75 for CD4/CD8 < 1 (95% CI: 0.58–0.91, P = 0.0212), and 0.77 for CD8 T cells ≥ 500/µL (95% CI: 0.63–0.92, P = 0.0096). In addition, PCA results suggest that large EVs miR-155 copies may be a biomarker of immune activation. Since miR-155 may influence immune cell function, its enrichment in large EV subpopulations could be a functional biomarker of immune activation in PLWH on ART. This measure could help to monitor and diagnose the immune activation with more accuracy. |
format | Online Article Text |
id | pubmed-9464867 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94648672022-09-13 Plasma vesicular miR-155 as a biomarker of immune activation in antiretroviral treated people living with HIV Bazié, Wilfried Wenceslas Boucher, Julien Goyer, Benjamin Traoré, Isidore Tiandiogo Kania, Dramane Somé, Diane Yirgnur Alary, Michel Gilbert, Caroline Front Immunol Immunology People living with HIV (PLWH), despite suppression of viral replication with antiretroviral therapy (ART), have high morbidity and mortality due to immune activation and chronic inflammation. Discovering new biomarkers of immune activation status under ART will be pertinent to improve PLWH quality of life when the majority will be treated. We stipulate that plasma large and small extracellular vesicle (EVs) and their microRNA content could be easily measured biomarkers to monitor immune activation in PLWH. Venous blood samples from n = 128 ART-treated PLWH with suppressed viral load (≤ 20 copies/mL) and n = 60 HIV-uninfected participants were collected at five testing or treatment centers of PLWH in Burkina Faso. Large and small plasma EVs were purified, counted, and the mature miRNAs miR-29a, miR-146a, and miR-155 were quantified by RT-qPCR. Diagnostic performances of large and small EVs miRNAs level were evaluated by receiver operating characteristic (ROC) curve analysis and principal component analysis (PCA). Among the EVs microRNA measured, only large EVs miR-155 copies distinguished PLWH with immune activation, with AUC of 0.75 for CD4/CD8 < 1 (95% CI: 0.58–0.91, P = 0.0212), and 0.77 for CD8 T cells ≥ 500/µL (95% CI: 0.63–0.92, P = 0.0096). In addition, PCA results suggest that large EVs miR-155 copies may be a biomarker of immune activation. Since miR-155 may influence immune cell function, its enrichment in large EV subpopulations could be a functional biomarker of immune activation in PLWH on ART. This measure could help to monitor and diagnose the immune activation with more accuracy. Frontiers Media S.A. 2022-08-29 /pmc/articles/PMC9464867/ /pubmed/36105810 http://dx.doi.org/10.3389/fimmu.2022.916599 Text en Copyright © 2022 Bazié, Boucher, Goyer, Traoré, Kania, Somé, Alary and Gilbert https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Bazié, Wilfried Wenceslas Boucher, Julien Goyer, Benjamin Traoré, Isidore Tiandiogo Kania, Dramane Somé, Diane Yirgnur Alary, Michel Gilbert, Caroline Plasma vesicular miR-155 as a biomarker of immune activation in antiretroviral treated people living with HIV |
title | Plasma vesicular miR-155 as a biomarker of immune activation in antiretroviral treated people living with HIV |
title_full | Plasma vesicular miR-155 as a biomarker of immune activation in antiretroviral treated people living with HIV |
title_fullStr | Plasma vesicular miR-155 as a biomarker of immune activation in antiretroviral treated people living with HIV |
title_full_unstemmed | Plasma vesicular miR-155 as a biomarker of immune activation in antiretroviral treated people living with HIV |
title_short | Plasma vesicular miR-155 as a biomarker of immune activation in antiretroviral treated people living with HIV |
title_sort | plasma vesicular mir-155 as a biomarker of immune activation in antiretroviral treated people living with hiv |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9464867/ https://www.ncbi.nlm.nih.gov/pubmed/36105810 http://dx.doi.org/10.3389/fimmu.2022.916599 |
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