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Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential

Remaining challenges in auricular cartilage tissue engineering include acquiring sufficient amounts of regeneration-competent cells and subsequent production of high-quality neocartilage. Progenitor cells are a resident subpopulation of native cartilage, displaying a high proliferative and cartilage...

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Autores principales: Otto, Iris A., Bernal, Paulina Nuñez, Rikkers, Margot, van Rijen, Mattie H.P., Mensinga, Anneloes, Kon, Moshe, Breugem, Corstiaan C., Levato, Riccardo, Malda, Jos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9464889/
https://www.ncbi.nlm.nih.gov/pubmed/36105583
http://dx.doi.org/10.1016/j.isci.2022.104979
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author Otto, Iris A.
Bernal, Paulina Nuñez
Rikkers, Margot
van Rijen, Mattie H.P.
Mensinga, Anneloes
Kon, Moshe
Breugem, Corstiaan C.
Levato, Riccardo
Malda, Jos
author_facet Otto, Iris A.
Bernal, Paulina Nuñez
Rikkers, Margot
van Rijen, Mattie H.P.
Mensinga, Anneloes
Kon, Moshe
Breugem, Corstiaan C.
Levato, Riccardo
Malda, Jos
author_sort Otto, Iris A.
collection PubMed
description Remaining challenges in auricular cartilage tissue engineering include acquiring sufficient amounts of regeneration-competent cells and subsequent production of high-quality neocartilage. Progenitor cells are a resident subpopulation of native cartilage, displaying a high proliferative and cartilage-forming capacity, yet their potential for regenerative medicine is vastly understudied. In this study, human auricular cartilage progenitor cells were newly identified in healthy cartilage and, importantly, in microtia-impaired chondral remnants. Their cartilage repair potential was assessed via in vitro 3D culture upon encapsulation in a gelatin-based hydrogel, and subsequent biochemical, mechanical, and histological analyses. Auricular cartilage progenitor cells demonstrate a potent ability to proliferate without losing their multipotent differentiation ability and to produce cartilage-like matrix in 3D culture. As these cells can be easily obtained through a non-deforming biopsy of the healthy ear or from the otherwise redundant microtia remnant, they can provide an important solution for long-existing challenges in auricular cartilage tissue engineering.
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spelling pubmed-94648892022-09-13 Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential Otto, Iris A. Bernal, Paulina Nuñez Rikkers, Margot van Rijen, Mattie H.P. Mensinga, Anneloes Kon, Moshe Breugem, Corstiaan C. Levato, Riccardo Malda, Jos iScience Article Remaining challenges in auricular cartilage tissue engineering include acquiring sufficient amounts of regeneration-competent cells and subsequent production of high-quality neocartilage. Progenitor cells are a resident subpopulation of native cartilage, displaying a high proliferative and cartilage-forming capacity, yet their potential for regenerative medicine is vastly understudied. In this study, human auricular cartilage progenitor cells were newly identified in healthy cartilage and, importantly, in microtia-impaired chondral remnants. Their cartilage repair potential was assessed via in vitro 3D culture upon encapsulation in a gelatin-based hydrogel, and subsequent biochemical, mechanical, and histological analyses. Auricular cartilage progenitor cells demonstrate a potent ability to proliferate without losing their multipotent differentiation ability and to produce cartilage-like matrix in 3D culture. As these cells can be easily obtained through a non-deforming biopsy of the healthy ear or from the otherwise redundant microtia remnant, they can provide an important solution for long-existing challenges in auricular cartilage tissue engineering. Elsevier 2022-08-18 /pmc/articles/PMC9464889/ /pubmed/36105583 http://dx.doi.org/10.1016/j.isci.2022.104979 Text en © 2022. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Otto, Iris A.
Bernal, Paulina Nuñez
Rikkers, Margot
van Rijen, Mattie H.P.
Mensinga, Anneloes
Kon, Moshe
Breugem, Corstiaan C.
Levato, Riccardo
Malda, Jos
Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential
title Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential
title_full Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential
title_fullStr Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential
title_full_unstemmed Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential
title_short Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential
title_sort human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9464889/
https://www.ncbi.nlm.nih.gov/pubmed/36105583
http://dx.doi.org/10.1016/j.isci.2022.104979
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