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Characteristics of salivary microbiota in children with obstructive sleep apnea: A prospective study with polysomnography

OBJECTIVES: The present study aimed to investigate the characteristics of salivary microbiota of children with obstructive sleep apnea (OSA) and to assess longitudinal alterations in salivary microbiota before and after adenotonsillectomy. METHODS: A set of cross-sectional samples consisted of 36 OS...

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Autores principales: Huang, Xin, Chen, Xuehui, Gong, Xu, Xu, Ying, Xu, Zhifei, Gao, Xuemei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9465092/
https://www.ncbi.nlm.nih.gov/pubmed/36105146
http://dx.doi.org/10.3389/fcimb.2022.945284
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author Huang, Xin
Chen, Xuehui
Gong, Xu
Xu, Ying
Xu, Zhifei
Gao, Xuemei
author_facet Huang, Xin
Chen, Xuehui
Gong, Xu
Xu, Ying
Xu, Zhifei
Gao, Xuemei
author_sort Huang, Xin
collection PubMed
description OBJECTIVES: The present study aimed to investigate the characteristics of salivary microbiota of children with obstructive sleep apnea (OSA) and to assess longitudinal alterations in salivary microbiota before and after adenotonsillectomy. METHODS: A set of cross-sectional samples consisted of 36 OSA children (17 boys and 19 girls, 7.47 ± 2.24 years old) and 22 controls (9 boys and 13 girls, 7.55 ± 2.48 years old) were included in the study, among which eight OSA children (five boys and three girls, 8.8 ± 2.0 years old) who underwent treatment of adenotonsillectomy were followed up after 1 year. Saliva samples were collected, and microbial profiles were analyzed by bioinformatics analysis based on 16S rRNA sequencing. RESULTS: In cross-sectional samples, the OSA group had higher α-diversity as estimated by Chao1, Shannon, Simpson, Pielou_e, and observed species as compared with the control group (p < 0.05). β-Diversity based on the Bray–Curtis dissimilarities (p = 0.004) and Jaccard distances (p = 0.001) revealed a significant separation between the OSA group and control group. Nested cross-validated random forest classifier identified the 10 most important genera (Lactobacillus, Escherichia, Bifidobacterium, Capnocytophaga, Bacteroidetes_[G-7], Parvimonas, Bacteroides, Klebsiella, Lautropia, and Prevotella) that could differentiate OSA children from controls with an area under the curve (AUC) of 0.94. Linear discriminant analysis effect size (LEfSe) analysis revealed a significantly higher abundance of genera such as Prevotella (p = 0.027), Actinomyces (p = 0.015), Bifidobacterium (p < 0.001), Escherichia (p < 0.001), and Lactobacillus (p < 0.001) in the OSA group, among which Prevotella was further corroborated in longitudinal samples. Prevotella sp_HMT_396 was found to be significantly enriched in the OSA group (p = 0.02) with significantly higher levels as OSA severity increased (p = 0.014), and it had a lower abundance in the post-treatment group (p = 0.003) with a decline in each OSA child 1 year after adenotonsillectomy. CONCLUSIONS: A significantly higher microbial diversity and a significant difference in microbial composition and abundance were identified in salivary microbiota of OSA children compared with controls. Meanwhile, some characteristic genera (Prevotella, Actinomyces, Lactobacillus, Escherichia, and Bifidobacterium) were found in OSA children, among which the relationship between Prevotella spp. and OSA is worth further studies.
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spelling pubmed-94650922022-09-13 Characteristics of salivary microbiota in children with obstructive sleep apnea: A prospective study with polysomnography Huang, Xin Chen, Xuehui Gong, Xu Xu, Ying Xu, Zhifei Gao, Xuemei Front Cell Infect Microbiol Cellular and Infection Microbiology OBJECTIVES: The present study aimed to investigate the characteristics of salivary microbiota of children with obstructive sleep apnea (OSA) and to assess longitudinal alterations in salivary microbiota before and after adenotonsillectomy. METHODS: A set of cross-sectional samples consisted of 36 OSA children (17 boys and 19 girls, 7.47 ± 2.24 years old) and 22 controls (9 boys and 13 girls, 7.55 ± 2.48 years old) were included in the study, among which eight OSA children (five boys and three girls, 8.8 ± 2.0 years old) who underwent treatment of adenotonsillectomy were followed up after 1 year. Saliva samples were collected, and microbial profiles were analyzed by bioinformatics analysis based on 16S rRNA sequencing. RESULTS: In cross-sectional samples, the OSA group had higher α-diversity as estimated by Chao1, Shannon, Simpson, Pielou_e, and observed species as compared with the control group (p < 0.05). β-Diversity based on the Bray–Curtis dissimilarities (p = 0.004) and Jaccard distances (p = 0.001) revealed a significant separation between the OSA group and control group. Nested cross-validated random forest classifier identified the 10 most important genera (Lactobacillus, Escherichia, Bifidobacterium, Capnocytophaga, Bacteroidetes_[G-7], Parvimonas, Bacteroides, Klebsiella, Lautropia, and Prevotella) that could differentiate OSA children from controls with an area under the curve (AUC) of 0.94. Linear discriminant analysis effect size (LEfSe) analysis revealed a significantly higher abundance of genera such as Prevotella (p = 0.027), Actinomyces (p = 0.015), Bifidobacterium (p < 0.001), Escherichia (p < 0.001), and Lactobacillus (p < 0.001) in the OSA group, among which Prevotella was further corroborated in longitudinal samples. Prevotella sp_HMT_396 was found to be significantly enriched in the OSA group (p = 0.02) with significantly higher levels as OSA severity increased (p = 0.014), and it had a lower abundance in the post-treatment group (p = 0.003) with a decline in each OSA child 1 year after adenotonsillectomy. CONCLUSIONS: A significantly higher microbial diversity and a significant difference in microbial composition and abundance were identified in salivary microbiota of OSA children compared with controls. Meanwhile, some characteristic genera (Prevotella, Actinomyces, Lactobacillus, Escherichia, and Bifidobacterium) were found in OSA children, among which the relationship between Prevotella spp. and OSA is worth further studies. Frontiers Media S.A. 2022-08-29 /pmc/articles/PMC9465092/ /pubmed/36105146 http://dx.doi.org/10.3389/fcimb.2022.945284 Text en Copyright © 2022 Huang, Chen, Gong, Xu, Xu and Gao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Huang, Xin
Chen, Xuehui
Gong, Xu
Xu, Ying
Xu, Zhifei
Gao, Xuemei
Characteristics of salivary microbiota in children with obstructive sleep apnea: A prospective study with polysomnography
title Characteristics of salivary microbiota in children with obstructive sleep apnea: A prospective study with polysomnography
title_full Characteristics of salivary microbiota in children with obstructive sleep apnea: A prospective study with polysomnography
title_fullStr Characteristics of salivary microbiota in children with obstructive sleep apnea: A prospective study with polysomnography
title_full_unstemmed Characteristics of salivary microbiota in children with obstructive sleep apnea: A prospective study with polysomnography
title_short Characteristics of salivary microbiota in children with obstructive sleep apnea: A prospective study with polysomnography
title_sort characteristics of salivary microbiota in children with obstructive sleep apnea: a prospective study with polysomnography
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9465092/
https://www.ncbi.nlm.nih.gov/pubmed/36105146
http://dx.doi.org/10.3389/fcimb.2022.945284
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