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Multi-omics insights reveal the remodeling of gut mycobiome with P. gingivalis

As a keystone periodontal pathogen, Porphyromonas gingivalis (P. gingivalis) was suggested to be involved in the progression of systemic diseases by altering the intestinal microecology. However, studies concerning gut microbiome have focused entirely on the bacterial component, while the fungal com...

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Autores principales: Chen, Si, Niu, ChenGuang, Lv, WanQi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9465408/
https://www.ncbi.nlm.nih.gov/pubmed/36105149
http://dx.doi.org/10.3389/fcimb.2022.937725
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author Chen, Si
Niu, ChenGuang
Lv, WanQi
author_facet Chen, Si
Niu, ChenGuang
Lv, WanQi
author_sort Chen, Si
collection PubMed
description As a keystone periodontal pathogen, Porphyromonas gingivalis (P. gingivalis) was suggested to be involved in the progression of systemic diseases by altering the intestinal microecology. However, studies concerning gut microbiome have focused entirely on the bacterial component, while the fungal community (gut mycobiome) has been overlooked. In this study, we aimed to characterize the alteration of gut mycobiome profile with P. gingivalis administration using mice fecal samples. Metagenomic analysis showed a distinct composition pattern of mycobiome and significant difference of beta diversity between control and the P. gingivalis group. Some fungal species were differentially characterized with P. gingivalis administration, among which Pyricularia pennisetigena and Alternaria alternata showed positive correlation with P. gingivalis. KEGG functional analyses revealed that three pathways, namely, “pentose and glucuronate interconversions”, “metabolic pathways”, and “two-component system”, were statistically enriched with P. gingivalis administration. Moreover, the alteration of gut mycobiome was also closely related with serum metabolites, especially lipid and tryptophan metabolic pathways. Taken together, this study demonstrated the alteration of fungal composition and function with P. gingivalis administration for the first time, and investigated the fungi–bacterial interaction and fungi–metabolite interaction preliminarily, providing a whole insight into gut mycobiome remodeling with oral pathobiont through multi-omics analyses.
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spelling pubmed-94654082022-09-13 Multi-omics insights reveal the remodeling of gut mycobiome with P. gingivalis Chen, Si Niu, ChenGuang Lv, WanQi Front Cell Infect Microbiol Cellular and Infection Microbiology As a keystone periodontal pathogen, Porphyromonas gingivalis (P. gingivalis) was suggested to be involved in the progression of systemic diseases by altering the intestinal microecology. However, studies concerning gut microbiome have focused entirely on the bacterial component, while the fungal community (gut mycobiome) has been overlooked. In this study, we aimed to characterize the alteration of gut mycobiome profile with P. gingivalis administration using mice fecal samples. Metagenomic analysis showed a distinct composition pattern of mycobiome and significant difference of beta diversity between control and the P. gingivalis group. Some fungal species were differentially characterized with P. gingivalis administration, among which Pyricularia pennisetigena and Alternaria alternata showed positive correlation with P. gingivalis. KEGG functional analyses revealed that three pathways, namely, “pentose and glucuronate interconversions”, “metabolic pathways”, and “two-component system”, were statistically enriched with P. gingivalis administration. Moreover, the alteration of gut mycobiome was also closely related with serum metabolites, especially lipid and tryptophan metabolic pathways. Taken together, this study demonstrated the alteration of fungal composition and function with P. gingivalis administration for the first time, and investigated the fungi–bacterial interaction and fungi–metabolite interaction preliminarily, providing a whole insight into gut mycobiome remodeling with oral pathobiont through multi-omics analyses. Frontiers Media S.A. 2022-08-29 /pmc/articles/PMC9465408/ /pubmed/36105149 http://dx.doi.org/10.3389/fcimb.2022.937725 Text en Copyright © 2022 Chen, Niu and Lv https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Chen, Si
Niu, ChenGuang
Lv, WanQi
Multi-omics insights reveal the remodeling of gut mycobiome with P. gingivalis
title Multi-omics insights reveal the remodeling of gut mycobiome with P. gingivalis
title_full Multi-omics insights reveal the remodeling of gut mycobiome with P. gingivalis
title_fullStr Multi-omics insights reveal the remodeling of gut mycobiome with P. gingivalis
title_full_unstemmed Multi-omics insights reveal the remodeling of gut mycobiome with P. gingivalis
title_short Multi-omics insights reveal the remodeling of gut mycobiome with P. gingivalis
title_sort multi-omics insights reveal the remodeling of gut mycobiome with p. gingivalis
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9465408/
https://www.ncbi.nlm.nih.gov/pubmed/36105149
http://dx.doi.org/10.3389/fcimb.2022.937725
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