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Dose Dependent Effects of Breynia cernua Against the Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: In Vitro, In Vivo and Mechanistic Studies
The aims and objectives of the study were to evaluate the antiParkinson’s (PD) potential of B cernua (BCE). B cernua (Poir.) Müll. Arg. (B cernua) is a member of the Phyllanthaceae family. HPLC revealed the presence of various phytochemicals. Study was conducted for 40 days. After PD induction by pa...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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SAGE Publications
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9465616/ https://www.ncbi.nlm.nih.gov/pubmed/36106058 http://dx.doi.org/10.1177/15593258221125478 |
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author | Saadullah, Malik Arif, Sania Hussain, Liaqat Asif, Muhammad Khurshid, Umair |
author_facet | Saadullah, Malik Arif, Sania Hussain, Liaqat Asif, Muhammad Khurshid, Umair |
author_sort | Saadullah, Malik |
collection | PubMed |
description | The aims and objectives of the study were to evaluate the antiParkinson’s (PD) potential of B cernua (BCE). B cernua (Poir.) Müll. Arg. (B cernua) is a member of the Phyllanthaceae family. HPLC revealed the presence of various phytochemicals. Study was conducted for 40 days. After PD induction by paraquat behavioural studies were carried out. Biochemical parameters such as DPPH, NO-scavenging, Ferrous reducing power, MDA, GSH, CAT, SOD, acetylcholinesterase (AChE), neurotransmitter estimation and TNF-α and IL-6 levels were determined. DPPH, NO-scavenging and Ferrous reducing power assays showed 78.02%, 48.05% and 71.45% inhibitions, respectively. There was significant improvement in motor functions and coordination in a dose-dependent manner (50 < 250 < 500 mg/kg) in PD rat model. Biochemical markers; SOD, CAT, GPx and GSH showed significant restoration (P < .001) while MDA showed significant decrease (P < .05). The AChE level was significantly reduced (P < .05) at 500 mg/kg while neurotransmitters were significantly improved (P < .001) in a dose-dependent fashion. The ELISA results showed significant (P < .001) down-regulation of IL-6 and TNF-α level. In conclusion, it is suggested that BCE has the potential to reduce the symptoms of PD. |
format | Online Article Text |
id | pubmed-9465616 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-94656162022-09-13 Dose Dependent Effects of Breynia cernua Against the Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: In Vitro, In Vivo and Mechanistic Studies Saadullah, Malik Arif, Sania Hussain, Liaqat Asif, Muhammad Khurshid, Umair Dose Response Original Article The aims and objectives of the study were to evaluate the antiParkinson’s (PD) potential of B cernua (BCE). B cernua (Poir.) Müll. Arg. (B cernua) is a member of the Phyllanthaceae family. HPLC revealed the presence of various phytochemicals. Study was conducted for 40 days. After PD induction by paraquat behavioural studies were carried out. Biochemical parameters such as DPPH, NO-scavenging, Ferrous reducing power, MDA, GSH, CAT, SOD, acetylcholinesterase (AChE), neurotransmitter estimation and TNF-α and IL-6 levels were determined. DPPH, NO-scavenging and Ferrous reducing power assays showed 78.02%, 48.05% and 71.45% inhibitions, respectively. There was significant improvement in motor functions and coordination in a dose-dependent manner (50 < 250 < 500 mg/kg) in PD rat model. Biochemical markers; SOD, CAT, GPx and GSH showed significant restoration (P < .001) while MDA showed significant decrease (P < .05). The AChE level was significantly reduced (P < .05) at 500 mg/kg while neurotransmitters were significantly improved (P < .001) in a dose-dependent fashion. The ELISA results showed significant (P < .001) down-regulation of IL-6 and TNF-α level. In conclusion, it is suggested that BCE has the potential to reduce the symptoms of PD. SAGE Publications 2022-09-09 /pmc/articles/PMC9465616/ /pubmed/36106058 http://dx.doi.org/10.1177/15593258221125478 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Saadullah, Malik Arif, Sania Hussain, Liaqat Asif, Muhammad Khurshid, Umair Dose Dependent Effects of Breynia cernua Against the Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: In Vitro, In Vivo and Mechanistic Studies |
title | Dose Dependent Effects of Breynia cernua Against the
Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: In
Vitro, In Vivo and Mechanistic
Studies |
title_full | Dose Dependent Effects of Breynia cernua Against the
Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: In
Vitro, In Vivo and Mechanistic
Studies |
title_fullStr | Dose Dependent Effects of Breynia cernua Against the
Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: In
Vitro, In Vivo and Mechanistic
Studies |
title_full_unstemmed | Dose Dependent Effects of Breynia cernua Against the
Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: In
Vitro, In Vivo and Mechanistic
Studies |
title_short | Dose Dependent Effects of Breynia cernua Against the
Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: In
Vitro, In Vivo and Mechanistic
Studies |
title_sort | dose dependent effects of breynia cernua against the
paraquat induced parkinsonism like symptoms in animals’ model: in
vitro, in vivo and mechanistic
studies |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9465616/ https://www.ncbi.nlm.nih.gov/pubmed/36106058 http://dx.doi.org/10.1177/15593258221125478 |
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