Cargando…

Dose Dependent Effects of Breynia cernua Against the Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: In Vitro, In Vivo and Mechanistic Studies

The aims and objectives of the study were to evaluate the antiParkinson’s (PD) potential of B cernua (BCE). B cernua (Poir.) Müll. Arg. (B cernua) is a member of the Phyllanthaceae family. HPLC revealed the presence of various phytochemicals. Study was conducted for 40 days. After PD induction by pa...

Descripción completa

Detalles Bibliográficos
Autores principales: Saadullah, Malik, Arif, Sania, Hussain, Liaqat, Asif, Muhammad, Khurshid, Umair
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9465616/
https://www.ncbi.nlm.nih.gov/pubmed/36106058
http://dx.doi.org/10.1177/15593258221125478
_version_ 1784787838041063424
author Saadullah, Malik
Arif, Sania
Hussain, Liaqat
Asif, Muhammad
Khurshid, Umair
author_facet Saadullah, Malik
Arif, Sania
Hussain, Liaqat
Asif, Muhammad
Khurshid, Umair
author_sort Saadullah, Malik
collection PubMed
description The aims and objectives of the study were to evaluate the antiParkinson’s (PD) potential of B cernua (BCE). B cernua (Poir.) Müll. Arg. (B cernua) is a member of the Phyllanthaceae family. HPLC revealed the presence of various phytochemicals. Study was conducted for 40 days. After PD induction by paraquat behavioural studies were carried out. Biochemical parameters such as DPPH, NO-scavenging, Ferrous reducing power, MDA, GSH, CAT, SOD, acetylcholinesterase (AChE), neurotransmitter estimation and TNF-α and IL-6 levels were determined. DPPH, NO-scavenging and Ferrous reducing power assays showed 78.02%, 48.05% and 71.45% inhibitions, respectively. There was significant improvement in motor functions and coordination in a dose-dependent manner (50 < 250 < 500 mg/kg) in PD rat model. Biochemical markers; SOD, CAT, GPx and GSH showed significant restoration (P < .001) while MDA showed significant decrease (P < .05). The AChE level was significantly reduced (P < .05) at 500 mg/kg while neurotransmitters were significantly improved (P < .001) in a dose-dependent fashion. The ELISA results showed significant (P < .001) down-regulation of IL-6 and TNF-α level. In conclusion, it is suggested that BCE has the potential to reduce the symptoms of PD.
format Online
Article
Text
id pubmed-9465616
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher SAGE Publications
record_format MEDLINE/PubMed
spelling pubmed-94656162022-09-13 Dose Dependent Effects of Breynia cernua Against the Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: In Vitro, In Vivo and Mechanistic Studies Saadullah, Malik Arif, Sania Hussain, Liaqat Asif, Muhammad Khurshid, Umair Dose Response Original Article The aims and objectives of the study were to evaluate the antiParkinson’s (PD) potential of B cernua (BCE). B cernua (Poir.) Müll. Arg. (B cernua) is a member of the Phyllanthaceae family. HPLC revealed the presence of various phytochemicals. Study was conducted for 40 days. After PD induction by paraquat behavioural studies were carried out. Biochemical parameters such as DPPH, NO-scavenging, Ferrous reducing power, MDA, GSH, CAT, SOD, acetylcholinesterase (AChE), neurotransmitter estimation and TNF-α and IL-6 levels were determined. DPPH, NO-scavenging and Ferrous reducing power assays showed 78.02%, 48.05% and 71.45% inhibitions, respectively. There was significant improvement in motor functions and coordination in a dose-dependent manner (50 < 250 < 500 mg/kg) in PD rat model. Biochemical markers; SOD, CAT, GPx and GSH showed significant restoration (P < .001) while MDA showed significant decrease (P < .05). The AChE level was significantly reduced (P < .05) at 500 mg/kg while neurotransmitters were significantly improved (P < .001) in a dose-dependent fashion. The ELISA results showed significant (P < .001) down-regulation of IL-6 and TNF-α level. In conclusion, it is suggested that BCE has the potential to reduce the symptoms of PD. SAGE Publications 2022-09-09 /pmc/articles/PMC9465616/ /pubmed/36106058 http://dx.doi.org/10.1177/15593258221125478 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Saadullah, Malik
Arif, Sania
Hussain, Liaqat
Asif, Muhammad
Khurshid, Umair
Dose Dependent Effects of Breynia cernua Against the Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: In Vitro, In Vivo and Mechanistic Studies
title Dose Dependent Effects of Breynia cernua Against the Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: In Vitro, In Vivo and Mechanistic Studies
title_full Dose Dependent Effects of Breynia cernua Against the Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: In Vitro, In Vivo and Mechanistic Studies
title_fullStr Dose Dependent Effects of Breynia cernua Against the Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: In Vitro, In Vivo and Mechanistic Studies
title_full_unstemmed Dose Dependent Effects of Breynia cernua Against the Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: In Vitro, In Vivo and Mechanistic Studies
title_short Dose Dependent Effects of Breynia cernua Against the Paraquat Induced Parkinsonism like Symptoms in Animals’ Model: In Vitro, In Vivo and Mechanistic Studies
title_sort dose dependent effects of breynia cernua against the paraquat induced parkinsonism like symptoms in animals’ model: in vitro, in vivo and mechanistic studies
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9465616/
https://www.ncbi.nlm.nih.gov/pubmed/36106058
http://dx.doi.org/10.1177/15593258221125478
work_keys_str_mv AT saadullahmalik dosedependenteffectsofbreyniacernuaagainsttheparaquatinducedparkinsonismlikesymptomsinanimalsmodelinvitroinvivoandmechanisticstudies
AT arifsania dosedependenteffectsofbreyniacernuaagainsttheparaquatinducedparkinsonismlikesymptomsinanimalsmodelinvitroinvivoandmechanisticstudies
AT hussainliaqat dosedependenteffectsofbreyniacernuaagainsttheparaquatinducedparkinsonismlikesymptomsinanimalsmodelinvitroinvivoandmechanisticstudies
AT asifmuhammad dosedependenteffectsofbreyniacernuaagainsttheparaquatinducedparkinsonismlikesymptomsinanimalsmodelinvitroinvivoandmechanisticstudies
AT khurshidumair dosedependenteffectsofbreyniacernuaagainsttheparaquatinducedparkinsonismlikesymptomsinanimalsmodelinvitroinvivoandmechanisticstudies