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Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea
To investigate the molecular pathological mechanisms of irritable bowel syndrome with diarrhea (IBS-D) and elucidate the effects of acupuncture on IBS-D colonic mucosa protein abundance in rats, a label-free high-throughput liquid chromatography-tandem mass spectrometry (LC-MS)-based proteomics anal...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467358/ https://www.ncbi.nlm.nih.gov/pubmed/36094925 http://dx.doi.org/10.1371/journal.pone.0273853 |
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author | Liu, Jing Peng, Rui Tan, Qian Li, Bocun Chen, Jianyi Liu, Guangya Wang, Yawen Li, Chao Li, Jia Wang, Hua |
author_facet | Liu, Jing Peng, Rui Tan, Qian Li, Bocun Chen, Jianyi Liu, Guangya Wang, Yawen Li, Chao Li, Jia Wang, Hua |
author_sort | Liu, Jing |
collection | PubMed |
description | To investigate the molecular pathological mechanisms of irritable bowel syndrome with diarrhea (IBS-D) and elucidate the effects of acupuncture on IBS-D colonic mucosa protein abundance in rats, a label-free high-throughput liquid chromatography-tandem mass spectrometry (LC-MS)-based proteomics analysis was used to survey the global changes of colonic mucosa proteins between different groups. Sixteen Sprague-Dawley (SD) male rats were randomly divided into four groups: the control group (C); the IBS-D model group (M); the syndrome differentiation acupuncture group (SD) and the traditional acupuncture group (T). IBS-D model rats were obtained using the CAS (chronic acute combining stress model) method. Comparative bioinformatics analysis of the proteomic data was analyzed using MaxQuant software, Perseus software, online tools DAVID, VENNY and STRING. Functional enrichment and network analyses revealed a close relationship between IBS-D and several biological processes including energy metabolism, muscular excitation/contraction, and both traditional acupuncture and syndrome differentiation acupuncture can reverse the impairments of normal energy metabolism. Moreover, the syndrome differentiation acupuncture can regulate the protein cluster relating inflammation, wound repair and cell protection against oxidative stress which is associated with acupuncture analgesic effect. Differentially expressed proteins Atp5a1 and Bpnt1 were selected as representative proteins and subjected to western blotting. In conclusion, our study provides further insight into the pathological and molecular mechanisms of IBS-D and acupuncture treatments, and serves as an experimental basis for clinical applications. |
format | Online Article Text |
id | pubmed-9467358 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-94673582022-09-13 Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea Liu, Jing Peng, Rui Tan, Qian Li, Bocun Chen, Jianyi Liu, Guangya Wang, Yawen Li, Chao Li, Jia Wang, Hua PLoS One Research Article To investigate the molecular pathological mechanisms of irritable bowel syndrome with diarrhea (IBS-D) and elucidate the effects of acupuncture on IBS-D colonic mucosa protein abundance in rats, a label-free high-throughput liquid chromatography-tandem mass spectrometry (LC-MS)-based proteomics analysis was used to survey the global changes of colonic mucosa proteins between different groups. Sixteen Sprague-Dawley (SD) male rats were randomly divided into four groups: the control group (C); the IBS-D model group (M); the syndrome differentiation acupuncture group (SD) and the traditional acupuncture group (T). IBS-D model rats were obtained using the CAS (chronic acute combining stress model) method. Comparative bioinformatics analysis of the proteomic data was analyzed using MaxQuant software, Perseus software, online tools DAVID, VENNY and STRING. Functional enrichment and network analyses revealed a close relationship between IBS-D and several biological processes including energy metabolism, muscular excitation/contraction, and both traditional acupuncture and syndrome differentiation acupuncture can reverse the impairments of normal energy metabolism. Moreover, the syndrome differentiation acupuncture can regulate the protein cluster relating inflammation, wound repair and cell protection against oxidative stress which is associated with acupuncture analgesic effect. Differentially expressed proteins Atp5a1 and Bpnt1 were selected as representative proteins and subjected to western blotting. In conclusion, our study provides further insight into the pathological and molecular mechanisms of IBS-D and acupuncture treatments, and serves as an experimental basis for clinical applications. Public Library of Science 2022-09-12 /pmc/articles/PMC9467358/ /pubmed/36094925 http://dx.doi.org/10.1371/journal.pone.0273853 Text en © 2022 Liu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Liu, Jing Peng, Rui Tan, Qian Li, Bocun Chen, Jianyi Liu, Guangya Wang, Yawen Li, Chao Li, Jia Wang, Hua Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea |
title | Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea |
title_full | Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea |
title_fullStr | Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea |
title_full_unstemmed | Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea |
title_short | Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea |
title_sort | proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467358/ https://www.ncbi.nlm.nih.gov/pubmed/36094925 http://dx.doi.org/10.1371/journal.pone.0273853 |
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