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Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea

To investigate the molecular pathological mechanisms of irritable bowel syndrome with diarrhea (IBS-D) and elucidate the effects of acupuncture on IBS-D colonic mucosa protein abundance in rats, a label-free high-throughput liquid chromatography-tandem mass spectrometry (LC-MS)-based proteomics anal...

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Autores principales: Liu, Jing, Peng, Rui, Tan, Qian, Li, Bocun, Chen, Jianyi, Liu, Guangya, Wang, Yawen, Li, Chao, Li, Jia, Wang, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467358/
https://www.ncbi.nlm.nih.gov/pubmed/36094925
http://dx.doi.org/10.1371/journal.pone.0273853
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author Liu, Jing
Peng, Rui
Tan, Qian
Li, Bocun
Chen, Jianyi
Liu, Guangya
Wang, Yawen
Li, Chao
Li, Jia
Wang, Hua
author_facet Liu, Jing
Peng, Rui
Tan, Qian
Li, Bocun
Chen, Jianyi
Liu, Guangya
Wang, Yawen
Li, Chao
Li, Jia
Wang, Hua
author_sort Liu, Jing
collection PubMed
description To investigate the molecular pathological mechanisms of irritable bowel syndrome with diarrhea (IBS-D) and elucidate the effects of acupuncture on IBS-D colonic mucosa protein abundance in rats, a label-free high-throughput liquid chromatography-tandem mass spectrometry (LC-MS)-based proteomics analysis was used to survey the global changes of colonic mucosa proteins between different groups. Sixteen Sprague-Dawley (SD) male rats were randomly divided into four groups: the control group (C); the IBS-D model group (M); the syndrome differentiation acupuncture group (SD) and the traditional acupuncture group (T). IBS-D model rats were obtained using the CAS (chronic acute combining stress model) method. Comparative bioinformatics analysis of the proteomic data was analyzed using MaxQuant software, Perseus software, online tools DAVID, VENNY and STRING. Functional enrichment and network analyses revealed a close relationship between IBS-D and several biological processes including energy metabolism, muscular excitation/contraction, and both traditional acupuncture and syndrome differentiation acupuncture can reverse the impairments of normal energy metabolism. Moreover, the syndrome differentiation acupuncture can regulate the protein cluster relating inflammation, wound repair and cell protection against oxidative stress which is associated with acupuncture analgesic effect. Differentially expressed proteins Atp5a1 and Bpnt1 were selected as representative proteins and subjected to western blotting. In conclusion, our study provides further insight into the pathological and molecular mechanisms of IBS-D and acupuncture treatments, and serves as an experimental basis for clinical applications.
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spelling pubmed-94673582022-09-13 Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea Liu, Jing Peng, Rui Tan, Qian Li, Bocun Chen, Jianyi Liu, Guangya Wang, Yawen Li, Chao Li, Jia Wang, Hua PLoS One Research Article To investigate the molecular pathological mechanisms of irritable bowel syndrome with diarrhea (IBS-D) and elucidate the effects of acupuncture on IBS-D colonic mucosa protein abundance in rats, a label-free high-throughput liquid chromatography-tandem mass spectrometry (LC-MS)-based proteomics analysis was used to survey the global changes of colonic mucosa proteins between different groups. Sixteen Sprague-Dawley (SD) male rats were randomly divided into four groups: the control group (C); the IBS-D model group (M); the syndrome differentiation acupuncture group (SD) and the traditional acupuncture group (T). IBS-D model rats were obtained using the CAS (chronic acute combining stress model) method. Comparative bioinformatics analysis of the proteomic data was analyzed using MaxQuant software, Perseus software, online tools DAVID, VENNY and STRING. Functional enrichment and network analyses revealed a close relationship between IBS-D and several biological processes including energy metabolism, muscular excitation/contraction, and both traditional acupuncture and syndrome differentiation acupuncture can reverse the impairments of normal energy metabolism. Moreover, the syndrome differentiation acupuncture can regulate the protein cluster relating inflammation, wound repair and cell protection against oxidative stress which is associated with acupuncture analgesic effect. Differentially expressed proteins Atp5a1 and Bpnt1 were selected as representative proteins and subjected to western blotting. In conclusion, our study provides further insight into the pathological and molecular mechanisms of IBS-D and acupuncture treatments, and serves as an experimental basis for clinical applications. Public Library of Science 2022-09-12 /pmc/articles/PMC9467358/ /pubmed/36094925 http://dx.doi.org/10.1371/journal.pone.0273853 Text en © 2022 Liu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Liu, Jing
Peng, Rui
Tan, Qian
Li, Bocun
Chen, Jianyi
Liu, Guangya
Wang, Yawen
Li, Chao
Li, Jia
Wang, Hua
Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea
title Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea
title_full Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea
title_fullStr Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea
title_full_unstemmed Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea
title_short Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea
title_sort proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467358/
https://www.ncbi.nlm.nih.gov/pubmed/36094925
http://dx.doi.org/10.1371/journal.pone.0273853
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