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Dicer-mediated miR-200b expression contributes to cell migratory/invasive abilities and cancer stem cells properties of breast cancer cells

Distant metastasis is the leading cause of death in patients with breast cancer. Despite considerable treatment advances, the clinical outcomes of patients with metastatic breast cancer remain poor. CSCs can self-renew, enhancing cancer progression and metastasis. Dicer, a microRNA (miRNA) processin...

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Autores principales: Hsu, Tung-Wei, Chen,, Hsin-An, Liao, Po-Hsiang, Su, Yen-Hao, Chiu, Ching-Feng, Huang, Chih-Yang, Lin, Yu-Jung, Hung, Chih-Chiang, Yeh, Ming-Hsin, Sung, Shian-Ying, Su, Chih-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467414/
https://www.ncbi.nlm.nih.gov/pubmed/35951366
http://dx.doi.org/10.18632/aging.204205
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author Hsu, Tung-Wei
Chen,, Hsin-An
Liao, Po-Hsiang
Su, Yen-Hao
Chiu, Ching-Feng
Huang, Chih-Yang
Lin, Yu-Jung
Hung, Chih-Chiang
Yeh, Ming-Hsin
Sung, Shian-Ying
Su, Chih-Ming
author_facet Hsu, Tung-Wei
Chen,, Hsin-An
Liao, Po-Hsiang
Su, Yen-Hao
Chiu, Ching-Feng
Huang, Chih-Yang
Lin, Yu-Jung
Hung, Chih-Chiang
Yeh, Ming-Hsin
Sung, Shian-Ying
Su, Chih-Ming
author_sort Hsu, Tung-Wei
collection PubMed
description Distant metastasis is the leading cause of death in patients with breast cancer. Despite considerable treatment advances, the clinical outcomes of patients with metastatic breast cancer remain poor. CSCs can self-renew, enhancing cancer progression and metastasis. Dicer, a microRNA (miRNA) processing–related enzyme, is required for miRNA maturation. Imbalanced Dicer expression may be pivotal in cancer progression. However, whether and how Dicer affects the stemness of metastatic breast cancer cells remains unclear. Here, we hypothesized that Dicer regulates the migration, invasion, and stemness of breast cancer cells. We established highly invasive cell lines (MCF-7/I-3 and MDA-MB-231/I-3) and observed that Dicer expression was conspicuously lower in the highly invasive cells than in the parental cells. The silencing of Dicer significantly enhanced the cell migratory/invasive abilities and CSCs properties of the breast cancer cells. Conversely, the overexpression of Dicer in the highly invasive cells reduced their migration, invasion, and CSCs properties. Our bioinformatics analyses demonstrated that low Dicer levels were correlated with increased breast cancer risk. Suppression of Dicer inhibited miR-200b expression, whereas miR-200b suppression recovered Dicer knockdown–induced migration, invasion, and cancer stem cells (CSCs) properties of the breast cancer cells. Thus, our findings reveal that Dicer is a crucial regulator of the migration, invasion, and CSCs properties of breast cancer cells and is significantly associated with poor survival in patients with breast cancer.
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spelling pubmed-94674142022-09-14 Dicer-mediated miR-200b expression contributes to cell migratory/invasive abilities and cancer stem cells properties of breast cancer cells Hsu, Tung-Wei Chen,, Hsin-An Liao, Po-Hsiang Su, Yen-Hao Chiu, Ching-Feng Huang, Chih-Yang Lin, Yu-Jung Hung, Chih-Chiang Yeh, Ming-Hsin Sung, Shian-Ying Su, Chih-Ming Aging (Albany NY) Research Paper Distant metastasis is the leading cause of death in patients with breast cancer. Despite considerable treatment advances, the clinical outcomes of patients with metastatic breast cancer remain poor. CSCs can self-renew, enhancing cancer progression and metastasis. Dicer, a microRNA (miRNA) processing–related enzyme, is required for miRNA maturation. Imbalanced Dicer expression may be pivotal in cancer progression. However, whether and how Dicer affects the stemness of metastatic breast cancer cells remains unclear. Here, we hypothesized that Dicer regulates the migration, invasion, and stemness of breast cancer cells. We established highly invasive cell lines (MCF-7/I-3 and MDA-MB-231/I-3) and observed that Dicer expression was conspicuously lower in the highly invasive cells than in the parental cells. The silencing of Dicer significantly enhanced the cell migratory/invasive abilities and CSCs properties of the breast cancer cells. Conversely, the overexpression of Dicer in the highly invasive cells reduced their migration, invasion, and CSCs properties. Our bioinformatics analyses demonstrated that low Dicer levels were correlated with increased breast cancer risk. Suppression of Dicer inhibited miR-200b expression, whereas miR-200b suppression recovered Dicer knockdown–induced migration, invasion, and cancer stem cells (CSCs) properties of the breast cancer cells. Thus, our findings reveal that Dicer is a crucial regulator of the migration, invasion, and CSCs properties of breast cancer cells and is significantly associated with poor survival in patients with breast cancer. Impact Journals 2022-08-08 /pmc/articles/PMC9467414/ /pubmed/35951366 http://dx.doi.org/10.18632/aging.204205 Text en Copyright: © 2022 Hsu et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Hsu, Tung-Wei
Chen,, Hsin-An
Liao, Po-Hsiang
Su, Yen-Hao
Chiu, Ching-Feng
Huang, Chih-Yang
Lin, Yu-Jung
Hung, Chih-Chiang
Yeh, Ming-Hsin
Sung, Shian-Ying
Su, Chih-Ming
Dicer-mediated miR-200b expression contributes to cell migratory/invasive abilities and cancer stem cells properties of breast cancer cells
title Dicer-mediated miR-200b expression contributes to cell migratory/invasive abilities and cancer stem cells properties of breast cancer cells
title_full Dicer-mediated miR-200b expression contributes to cell migratory/invasive abilities and cancer stem cells properties of breast cancer cells
title_fullStr Dicer-mediated miR-200b expression contributes to cell migratory/invasive abilities and cancer stem cells properties of breast cancer cells
title_full_unstemmed Dicer-mediated miR-200b expression contributes to cell migratory/invasive abilities and cancer stem cells properties of breast cancer cells
title_short Dicer-mediated miR-200b expression contributes to cell migratory/invasive abilities and cancer stem cells properties of breast cancer cells
title_sort dicer-mediated mir-200b expression contributes to cell migratory/invasive abilities and cancer stem cells properties of breast cancer cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467414/
https://www.ncbi.nlm.nih.gov/pubmed/35951366
http://dx.doi.org/10.18632/aging.204205
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