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The number of cytokinesis nodes in mitotic fission yeast scales with cell size

Cytokinesis nodes are assemblies of stoichiometric ratios of proteins associated with the plasma membrane, which serve as precursors for the contractile ring during cytokinesis by fission yeast. The total number of nodes is uncertain, because of the limitations of the methods used previously. Here,...

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Autores principales: Sayyad, Wasim A, Pollard, Thomas D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467510/
https://www.ncbi.nlm.nih.gov/pubmed/36093997
http://dx.doi.org/10.7554/eLife.76249
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author Sayyad, Wasim A
Pollard, Thomas D
author_facet Sayyad, Wasim A
Pollard, Thomas D
author_sort Sayyad, Wasim A
collection PubMed
description Cytokinesis nodes are assemblies of stoichiometric ratios of proteins associated with the plasma membrane, which serve as precursors for the contractile ring during cytokinesis by fission yeast. The total number of nodes is uncertain, because of the limitations of the methods used previously. Here, we used the ~140 nm resolution of Airyscan super-resolution microscopy to measure the fluorescence intensity of small, single cytokinesis nodes marked with Blt1-mEGFP in live fission yeast cells early in mitosis. The ratio of the total Blt1-mEGFP fluorescence in the broad band of cytokinesis nodes to the average fluorescence of a single node gives about 190 single cytokinesis nodes in wild-type fission yeast cells early in mitosis. Most, but not all of these nodes condense into a contractile ring. The number of cytokinesis nodes scales with cell size in four strains tested, although large diameter rga4Δ mutant cells form somewhat fewer cytokinesis nodes than expected from the overall trend. The Pom1 kinase restricts cytokinesis nodes from the ends of cells, but the surface density of Pom1 on the plasma membrane around the equators of cells is similar with a wide range of node numbers, so Pom1 does not control cytokinesis node number. However, when the concentrations of either kinase Pom1 or kinase Cdr2 were varied with the nmt1 promoter, the numbers of cytokinesis nodes increased above a baseline of about ~190 with the total cellular concentration of either kinase.
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spelling pubmed-94675102022-09-13 The number of cytokinesis nodes in mitotic fission yeast scales with cell size Sayyad, Wasim A Pollard, Thomas D eLife Cell Biology Cytokinesis nodes are assemblies of stoichiometric ratios of proteins associated with the plasma membrane, which serve as precursors for the contractile ring during cytokinesis by fission yeast. The total number of nodes is uncertain, because of the limitations of the methods used previously. Here, we used the ~140 nm resolution of Airyscan super-resolution microscopy to measure the fluorescence intensity of small, single cytokinesis nodes marked with Blt1-mEGFP in live fission yeast cells early in mitosis. The ratio of the total Blt1-mEGFP fluorescence in the broad band of cytokinesis nodes to the average fluorescence of a single node gives about 190 single cytokinesis nodes in wild-type fission yeast cells early in mitosis. Most, but not all of these nodes condense into a contractile ring. The number of cytokinesis nodes scales with cell size in four strains tested, although large diameter rga4Δ mutant cells form somewhat fewer cytokinesis nodes than expected from the overall trend. The Pom1 kinase restricts cytokinesis nodes from the ends of cells, but the surface density of Pom1 on the plasma membrane around the equators of cells is similar with a wide range of node numbers, so Pom1 does not control cytokinesis node number. However, when the concentrations of either kinase Pom1 or kinase Cdr2 were varied with the nmt1 promoter, the numbers of cytokinesis nodes increased above a baseline of about ~190 with the total cellular concentration of either kinase. eLife Sciences Publications, Ltd 2022-09-12 /pmc/articles/PMC9467510/ /pubmed/36093997 http://dx.doi.org/10.7554/eLife.76249 Text en © 2022, Sayyad and Pollard https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Sayyad, Wasim A
Pollard, Thomas D
The number of cytokinesis nodes in mitotic fission yeast scales with cell size
title The number of cytokinesis nodes in mitotic fission yeast scales with cell size
title_full The number of cytokinesis nodes in mitotic fission yeast scales with cell size
title_fullStr The number of cytokinesis nodes in mitotic fission yeast scales with cell size
title_full_unstemmed The number of cytokinesis nodes in mitotic fission yeast scales with cell size
title_short The number of cytokinesis nodes in mitotic fission yeast scales with cell size
title_sort number of cytokinesis nodes in mitotic fission yeast scales with cell size
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467510/
https://www.ncbi.nlm.nih.gov/pubmed/36093997
http://dx.doi.org/10.7554/eLife.76249
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