Cargando…
Characterization of Chitinolytic and Antifungal Activities in Marine-Derived Trichoderma bissettii Strains
Trichoderma fungi have been intensively studied for mycoparasitism, and the latter is closely related to their cell-wall degrading enzymes including chitinase. Here, we studied marine-derived Trichoderma spp., isolated from distinct sources and locations, for chitinolytic and antifungal activity. Ba...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467547/ https://www.ncbi.nlm.nih.gov/pubmed/36158047 http://dx.doi.org/10.1080/12298093.2022.2105509 |
_version_ | 1784788215878647808 |
---|---|
author | Chung, Dawoon Kwon, Yong Min Lim, Ji Yeon Bae, Seung Sub Choi, Grace Lee, Dae-Sung |
author_facet | Chung, Dawoon Kwon, Yong Min Lim, Ji Yeon Bae, Seung Sub Choi, Grace Lee, Dae-Sung |
author_sort | Chung, Dawoon |
collection | PubMed |
description | Trichoderma fungi have been intensively studied for mycoparasitism, and the latter is closely related to their cell-wall degrading enzymes including chitinase. Here, we studied marine-derived Trichoderma spp., isolated from distinct sources and locations, for chitinolytic and antifungal activity. Based on morphological and phylogenetic analyses, two strains designated GJ-Sp1 and TOP-Co8 (isolated from a marine sponge and a marine alga, respectively) were identified as Trichoderma bissettii. This species has recently been identified as a closely related species to Trichoderma longibrachiatum. The extracellular crude enzymes of GJ-Sp1 and TOP-Co8 showed activities of chitobiosidase and β-N-acetylglucosaminidase (exochitinase) and chitotriosidase (endochitinase). The optimum chitinolytic activity of the crude enzymes was observed at 50 °C, pH 5.0, 0–0.5% NaCl concentrations, and the activities were stable at temperatures ranging from 10 to 40 °C for 2 h. Moreover, the crude enzymes showed inhibitory activity against hyphal growth of two filamentous fungi Aspergillus flavus and Aspergillus niger. To the best of our knowledge, this is the first report of the chitinolytic and antifungal activity of T. bissettii. |
format | Online Article Text |
id | pubmed-9467547 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-94675472022-09-23 Characterization of Chitinolytic and Antifungal Activities in Marine-Derived Trichoderma bissettii Strains Chung, Dawoon Kwon, Yong Min Lim, Ji Yeon Bae, Seung Sub Choi, Grace Lee, Dae-Sung Mycobiology Research Articles Trichoderma fungi have been intensively studied for mycoparasitism, and the latter is closely related to their cell-wall degrading enzymes including chitinase. Here, we studied marine-derived Trichoderma spp., isolated from distinct sources and locations, for chitinolytic and antifungal activity. Based on morphological and phylogenetic analyses, two strains designated GJ-Sp1 and TOP-Co8 (isolated from a marine sponge and a marine alga, respectively) were identified as Trichoderma bissettii. This species has recently been identified as a closely related species to Trichoderma longibrachiatum. The extracellular crude enzymes of GJ-Sp1 and TOP-Co8 showed activities of chitobiosidase and β-N-acetylglucosaminidase (exochitinase) and chitotriosidase (endochitinase). The optimum chitinolytic activity of the crude enzymes was observed at 50 °C, pH 5.0, 0–0.5% NaCl concentrations, and the activities were stable at temperatures ranging from 10 to 40 °C for 2 h. Moreover, the crude enzymes showed inhibitory activity against hyphal growth of two filamentous fungi Aspergillus flavus and Aspergillus niger. To the best of our knowledge, this is the first report of the chitinolytic and antifungal activity of T. bissettii. Taylor & Francis 2022-08-24 /pmc/articles/PMC9467547/ /pubmed/36158047 http://dx.doi.org/10.1080/12298093.2022.2105509 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Chung, Dawoon Kwon, Yong Min Lim, Ji Yeon Bae, Seung Sub Choi, Grace Lee, Dae-Sung Characterization of Chitinolytic and Antifungal Activities in Marine-Derived Trichoderma bissettii Strains |
title | Characterization of Chitinolytic and Antifungal Activities in Marine-Derived Trichoderma bissettii Strains |
title_full | Characterization of Chitinolytic and Antifungal Activities in Marine-Derived Trichoderma bissettii Strains |
title_fullStr | Characterization of Chitinolytic and Antifungal Activities in Marine-Derived Trichoderma bissettii Strains |
title_full_unstemmed | Characterization of Chitinolytic and Antifungal Activities in Marine-Derived Trichoderma bissettii Strains |
title_short | Characterization of Chitinolytic and Antifungal Activities in Marine-Derived Trichoderma bissettii Strains |
title_sort | characterization of chitinolytic and antifungal activities in marine-derived trichoderma bissettii strains |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467547/ https://www.ncbi.nlm.nih.gov/pubmed/36158047 http://dx.doi.org/10.1080/12298093.2022.2105509 |
work_keys_str_mv | AT chungdawoon characterizationofchitinolyticandantifungalactivitiesinmarinederivedtrichodermabissettiistrains AT kwonyongmin characterizationofchitinolyticandantifungalactivitiesinmarinederivedtrichodermabissettiistrains AT limjiyeon characterizationofchitinolyticandantifungalactivitiesinmarinederivedtrichodermabissettiistrains AT baeseungsub characterizationofchitinolyticandantifungalactivitiesinmarinederivedtrichodermabissettiistrains AT choigrace characterizationofchitinolyticandantifungalactivitiesinmarinederivedtrichodermabissettiistrains AT leedaesung characterizationofchitinolyticandantifungalactivitiesinmarinederivedtrichodermabissettiistrains |