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Sporophytic control of pollen meiotic progression is mediated by tapetum expression of ABORTED MICROSPORES

Pollen development is dependent on the tapetum, a sporophytic anther cell layer surrounding the microspores that functions in pollen wall formation but is also essential for meiosis-associated development. There is clear evidence of crosstalk and co-regulation between the tapetum and microspores, bu...

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Autores principales: Tidy, Alison C, Ferjentsikova, Ivana, Vizcay-Barrena, Gema, Liu, Bing, Yin, Wenzhe, Higgins, James D, Xu, Jie, Zhang, Dabing, Geelen, Danny, Wilson, Zoe A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467646/
https://www.ncbi.nlm.nih.gov/pubmed/35617147
http://dx.doi.org/10.1093/jxb/erac225
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author Tidy, Alison C
Ferjentsikova, Ivana
Vizcay-Barrena, Gema
Liu, Bing
Yin, Wenzhe
Higgins, James D
Xu, Jie
Zhang, Dabing
Geelen, Danny
Wilson, Zoe A
author_facet Tidy, Alison C
Ferjentsikova, Ivana
Vizcay-Barrena, Gema
Liu, Bing
Yin, Wenzhe
Higgins, James D
Xu, Jie
Zhang, Dabing
Geelen, Danny
Wilson, Zoe A
author_sort Tidy, Alison C
collection PubMed
description Pollen development is dependent on the tapetum, a sporophytic anther cell layer surrounding the microspores that functions in pollen wall formation but is also essential for meiosis-associated development. There is clear evidence of crosstalk and co-regulation between the tapetum and microspores, but how this is achieved is currently not characterized. ABORTED MICROSPORES (AMS), a tapetum transcription factor, is important for pollen wall formation, but also has an undefined role in early pollen development. We conducted a detailed investigation of chromosome behaviour, cytokinesis, radial microtubule array (RMA) organization, and callose formation in the ams mutant. Early meiosis initiates normally in ams, shows delayed progression after the pachytene stage, and then fails during late meiosis, with disorganized RMA, defective cytokinesis, abnormal callose formation, and microspore degeneration, alongside abnormal tapetum development. Here, we show that selected meiosis-associated genes are directly repressed by AMS, and that AMS is essential for late meiosis progression. Our findings indicate that AMS has a dual function in tapetum–meiocyte crosstalk by playing an important regulatory role during late meiosis, in addition to its previously characterized role in pollen wall formation. AMS is critical for RMA organization, callose deposition, and therefore cytokinesis, and is involved in the crosstalk between the gametophyte and sporophytic tissues, which enables synchronous development of tapetum and microspores.
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spelling pubmed-94676462022-09-13 Sporophytic control of pollen meiotic progression is mediated by tapetum expression of ABORTED MICROSPORES Tidy, Alison C Ferjentsikova, Ivana Vizcay-Barrena, Gema Liu, Bing Yin, Wenzhe Higgins, James D Xu, Jie Zhang, Dabing Geelen, Danny Wilson, Zoe A J Exp Bot Research Papers Pollen development is dependent on the tapetum, a sporophytic anther cell layer surrounding the microspores that functions in pollen wall formation but is also essential for meiosis-associated development. There is clear evidence of crosstalk and co-regulation between the tapetum and microspores, but how this is achieved is currently not characterized. ABORTED MICROSPORES (AMS), a tapetum transcription factor, is important for pollen wall formation, but also has an undefined role in early pollen development. We conducted a detailed investigation of chromosome behaviour, cytokinesis, radial microtubule array (RMA) organization, and callose formation in the ams mutant. Early meiosis initiates normally in ams, shows delayed progression after the pachytene stage, and then fails during late meiosis, with disorganized RMA, defective cytokinesis, abnormal callose formation, and microspore degeneration, alongside abnormal tapetum development. Here, we show that selected meiosis-associated genes are directly repressed by AMS, and that AMS is essential for late meiosis progression. Our findings indicate that AMS has a dual function in tapetum–meiocyte crosstalk by playing an important regulatory role during late meiosis, in addition to its previously characterized role in pollen wall formation. AMS is critical for RMA organization, callose deposition, and therefore cytokinesis, and is involved in the crosstalk between the gametophyte and sporophytic tissues, which enables synchronous development of tapetum and microspores. Oxford University Press 2022-05-25 /pmc/articles/PMC9467646/ /pubmed/35617147 http://dx.doi.org/10.1093/jxb/erac225 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Tidy, Alison C
Ferjentsikova, Ivana
Vizcay-Barrena, Gema
Liu, Bing
Yin, Wenzhe
Higgins, James D
Xu, Jie
Zhang, Dabing
Geelen, Danny
Wilson, Zoe A
Sporophytic control of pollen meiotic progression is mediated by tapetum expression of ABORTED MICROSPORES
title Sporophytic control of pollen meiotic progression is mediated by tapetum expression of ABORTED MICROSPORES
title_full Sporophytic control of pollen meiotic progression is mediated by tapetum expression of ABORTED MICROSPORES
title_fullStr Sporophytic control of pollen meiotic progression is mediated by tapetum expression of ABORTED MICROSPORES
title_full_unstemmed Sporophytic control of pollen meiotic progression is mediated by tapetum expression of ABORTED MICROSPORES
title_short Sporophytic control of pollen meiotic progression is mediated by tapetum expression of ABORTED MICROSPORES
title_sort sporophytic control of pollen meiotic progression is mediated by tapetum expression of aborted microspores
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467646/
https://www.ncbi.nlm.nih.gov/pubmed/35617147
http://dx.doi.org/10.1093/jxb/erac225
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