Cargando…
The transcription factor TaMYB31 regulates the benzoxazinoid biosynthetic pathway in wheat
Benzoxazinoids are specialized metabolites that are highly abundant in staple crops, such as maize and wheat. Although their biosynthesis has been studied for several decades, the regulatory mechanisms of the benzoxazinoid pathway remain unknown. Here, we report that the wheat transcription factor M...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467655/ https://www.ncbi.nlm.nih.gov/pubmed/35554544 http://dx.doi.org/10.1093/jxb/erac204 |
_version_ | 1784788236408717312 |
---|---|
author | Batyrshina, Zhaniya S Shavit, Reut Yaakov, Beery Bocobza, Samuel Tzin, Vered |
author_facet | Batyrshina, Zhaniya S Shavit, Reut Yaakov, Beery Bocobza, Samuel Tzin, Vered |
author_sort | Batyrshina, Zhaniya S |
collection | PubMed |
description | Benzoxazinoids are specialized metabolites that are highly abundant in staple crops, such as maize and wheat. Although their biosynthesis has been studied for several decades, the regulatory mechanisms of the benzoxazinoid pathway remain unknown. Here, we report that the wheat transcription factor MYB31 functions as a regulator of benzoxazinoid biosynthesis genes. A transcriptomic analysis of tetraploid wheat (Triticum turgidum) tissue revealed the up-regulation of two TtMYB31 homoeologous genes upon aphid and caterpillar feeding. TaMYB31 gene silencing in the hexaploid wheat Triticum aestivum significantly reduced benzoxazinoid metabolite levels and led to susceptibility to herbivores. Thus, aphid progeny production, caterpillar body weight gain, and spider mite oviposition significantly increased in TaMYB31-silenced plants. A comprehensive transcriptomic analysis of hexaploid wheat revealed that the TaMYB31 gene is co-expressed with the target benzoxazinoid-encoded Bx genes under several biotic and environmental conditions. Therefore, we analyzed the effect of abiotic stresses on benzoxazinoid levels and discovered a strong accumulation of these compounds in the leaves. The results of a dual fluorescence assay indicated that TaMYB31 binds to the Bx1 and Bx4 gene promoters, thereby activating the transcription of genes involved in the benzoxazinoid pathway. Our finding is the first report of the transcriptional regulation mechanism of the benzoxazinoid pathway in wheat. |
format | Online Article Text |
id | pubmed-9467655 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-94676552022-09-13 The transcription factor TaMYB31 regulates the benzoxazinoid biosynthetic pathway in wheat Batyrshina, Zhaniya S Shavit, Reut Yaakov, Beery Bocobza, Samuel Tzin, Vered J Exp Bot Research Papers Benzoxazinoids are specialized metabolites that are highly abundant in staple crops, such as maize and wheat. Although their biosynthesis has been studied for several decades, the regulatory mechanisms of the benzoxazinoid pathway remain unknown. Here, we report that the wheat transcription factor MYB31 functions as a regulator of benzoxazinoid biosynthesis genes. A transcriptomic analysis of tetraploid wheat (Triticum turgidum) tissue revealed the up-regulation of two TtMYB31 homoeologous genes upon aphid and caterpillar feeding. TaMYB31 gene silencing in the hexaploid wheat Triticum aestivum significantly reduced benzoxazinoid metabolite levels and led to susceptibility to herbivores. Thus, aphid progeny production, caterpillar body weight gain, and spider mite oviposition significantly increased in TaMYB31-silenced plants. A comprehensive transcriptomic analysis of hexaploid wheat revealed that the TaMYB31 gene is co-expressed with the target benzoxazinoid-encoded Bx genes under several biotic and environmental conditions. Therefore, we analyzed the effect of abiotic stresses on benzoxazinoid levels and discovered a strong accumulation of these compounds in the leaves. The results of a dual fluorescence assay indicated that TaMYB31 binds to the Bx1 and Bx4 gene promoters, thereby activating the transcription of genes involved in the benzoxazinoid pathway. Our finding is the first report of the transcriptional regulation mechanism of the benzoxazinoid pathway in wheat. Oxford University Press 2022-05-12 /pmc/articles/PMC9467655/ /pubmed/35554544 http://dx.doi.org/10.1093/jxb/erac204 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Batyrshina, Zhaniya S Shavit, Reut Yaakov, Beery Bocobza, Samuel Tzin, Vered The transcription factor TaMYB31 regulates the benzoxazinoid biosynthetic pathway in wheat |
title | The transcription factor TaMYB31 regulates the benzoxazinoid biosynthetic pathway in wheat |
title_full | The transcription factor TaMYB31 regulates the benzoxazinoid biosynthetic pathway in wheat |
title_fullStr | The transcription factor TaMYB31 regulates the benzoxazinoid biosynthetic pathway in wheat |
title_full_unstemmed | The transcription factor TaMYB31 regulates the benzoxazinoid biosynthetic pathway in wheat |
title_short | The transcription factor TaMYB31 regulates the benzoxazinoid biosynthetic pathway in wheat |
title_sort | transcription factor tamyb31 regulates the benzoxazinoid biosynthetic pathway in wheat |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467655/ https://www.ncbi.nlm.nih.gov/pubmed/35554544 http://dx.doi.org/10.1093/jxb/erac204 |
work_keys_str_mv | AT batyrshinazhaniyas thetranscriptionfactortamyb31regulatesthebenzoxazinoidbiosyntheticpathwayinwheat AT shavitreut thetranscriptionfactortamyb31regulatesthebenzoxazinoidbiosyntheticpathwayinwheat AT yaakovbeery thetranscriptionfactortamyb31regulatesthebenzoxazinoidbiosyntheticpathwayinwheat AT bocobzasamuel thetranscriptionfactortamyb31regulatesthebenzoxazinoidbiosyntheticpathwayinwheat AT tzinvered thetranscriptionfactortamyb31regulatesthebenzoxazinoidbiosyntheticpathwayinwheat AT batyrshinazhaniyas transcriptionfactortamyb31regulatesthebenzoxazinoidbiosyntheticpathwayinwheat AT shavitreut transcriptionfactortamyb31regulatesthebenzoxazinoidbiosyntheticpathwayinwheat AT yaakovbeery transcriptionfactortamyb31regulatesthebenzoxazinoidbiosyntheticpathwayinwheat AT bocobzasamuel transcriptionfactortamyb31regulatesthebenzoxazinoidbiosyntheticpathwayinwheat AT tzinvered transcriptionfactortamyb31regulatesthebenzoxazinoidbiosyntheticpathwayinwheat |