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A Potential Anticancer Mechanism of Finger Root (Boesenbergia rotunda) Extracts against a Breast Cancer Cell Line

Breast cancer is the most common type of cancer women suffer from worldwide in 2020 and the 4th leading cause of cancer death. Boesenbergia rotunda is an herb with high potential as an anticancer agent. This study explores the potential bioactive compounds in B. rotunda as anti-breast cancer agents...

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Autores principales: Widyananda, Muhammad Hermawan, Wicaksono, Septian Tri, Rahmawati, Kurnia, Puspitarini, Sapti, Ulfa, Siti Mariyah, Jatmiko, Yoga Dwi, Masruri, Masruri, Widodo, Nashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467824/
https://www.ncbi.nlm.nih.gov/pubmed/36106139
http://dx.doi.org/10.1155/2022/9130252
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author Widyananda, Muhammad Hermawan
Wicaksono, Septian Tri
Rahmawati, Kurnia
Puspitarini, Sapti
Ulfa, Siti Mariyah
Jatmiko, Yoga Dwi
Masruri, Masruri
Widodo, Nashi
author_facet Widyananda, Muhammad Hermawan
Wicaksono, Septian Tri
Rahmawati, Kurnia
Puspitarini, Sapti
Ulfa, Siti Mariyah
Jatmiko, Yoga Dwi
Masruri, Masruri
Widodo, Nashi
author_sort Widyananda, Muhammad Hermawan
collection PubMed
description Breast cancer is the most common type of cancer women suffer from worldwide in 2020 and the 4th leading cause of cancer death. Boesenbergia rotunda is an herb with high potential as an anticancer agent. This study explores the potential bioactive compounds in B. rotunda as anti-breast cancer agents using in silico and in vitro approaches. The in silico study was used for active compound analysis, selection of anticancer compound candidates, prediction of target protein, functional annotation, molecular docking, and molecular dynamics simulation, respectively. The in vitro study was conducted by measurement toxicity, rhodamine 123, and apoptosis assays on T47D cells. Based on the KNApSAcK database, B. rotunda contained 20 metabolites, which are dominated by chalcone and flavonoid groups. Seven of them were predicted to have anticancer activity, namely, sakuranetin, cardamonin, alpinetin, 2S-pinocembrin, 7.4′-dihydroxy-5-methoxyflavanone, 5.6-dehydrokawain, and pinostrobin chalcone. These compounds targeted proteins related to cancer progression pathways such as the PI3K/Akt, FOXO, JAK/STAT, and estrogen signaling pathways. Therefore, these compounds are predicted to inhibit growth and induce apoptosis of cancer cells through their interactions with MMP12, MMP13, CDK4, JAK3, VEGFR1, VEGFR2, and KCNA3. Anticancer activity of B. rotunda through in vitro study confirmed that B. rotunda extract is strong cytotoxic and induces apoptosis of breast cancer cell lines. This study concludes that Boesenbergia rotunda has potency as an anticancer candidate.
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spelling pubmed-94678242022-09-13 A Potential Anticancer Mechanism of Finger Root (Boesenbergia rotunda) Extracts against a Breast Cancer Cell Line Widyananda, Muhammad Hermawan Wicaksono, Septian Tri Rahmawati, Kurnia Puspitarini, Sapti Ulfa, Siti Mariyah Jatmiko, Yoga Dwi Masruri, Masruri Widodo, Nashi Scientifica (Cairo) Research Article Breast cancer is the most common type of cancer women suffer from worldwide in 2020 and the 4th leading cause of cancer death. Boesenbergia rotunda is an herb with high potential as an anticancer agent. This study explores the potential bioactive compounds in B. rotunda as anti-breast cancer agents using in silico and in vitro approaches. The in silico study was used for active compound analysis, selection of anticancer compound candidates, prediction of target protein, functional annotation, molecular docking, and molecular dynamics simulation, respectively. The in vitro study was conducted by measurement toxicity, rhodamine 123, and apoptosis assays on T47D cells. Based on the KNApSAcK database, B. rotunda contained 20 metabolites, which are dominated by chalcone and flavonoid groups. Seven of them were predicted to have anticancer activity, namely, sakuranetin, cardamonin, alpinetin, 2S-pinocembrin, 7.4′-dihydroxy-5-methoxyflavanone, 5.6-dehydrokawain, and pinostrobin chalcone. These compounds targeted proteins related to cancer progression pathways such as the PI3K/Akt, FOXO, JAK/STAT, and estrogen signaling pathways. Therefore, these compounds are predicted to inhibit growth and induce apoptosis of cancer cells through their interactions with MMP12, MMP13, CDK4, JAK3, VEGFR1, VEGFR2, and KCNA3. Anticancer activity of B. rotunda through in vitro study confirmed that B. rotunda extract is strong cytotoxic and induces apoptosis of breast cancer cell lines. This study concludes that Boesenbergia rotunda has potency as an anticancer candidate. Hindawi 2022-09-05 /pmc/articles/PMC9467824/ /pubmed/36106139 http://dx.doi.org/10.1155/2022/9130252 Text en Copyright © 2022 Muhammad Hermawan Widyananda et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Widyananda, Muhammad Hermawan
Wicaksono, Septian Tri
Rahmawati, Kurnia
Puspitarini, Sapti
Ulfa, Siti Mariyah
Jatmiko, Yoga Dwi
Masruri, Masruri
Widodo, Nashi
A Potential Anticancer Mechanism of Finger Root (Boesenbergia rotunda) Extracts against a Breast Cancer Cell Line
title A Potential Anticancer Mechanism of Finger Root (Boesenbergia rotunda) Extracts against a Breast Cancer Cell Line
title_full A Potential Anticancer Mechanism of Finger Root (Boesenbergia rotunda) Extracts against a Breast Cancer Cell Line
title_fullStr A Potential Anticancer Mechanism of Finger Root (Boesenbergia rotunda) Extracts against a Breast Cancer Cell Line
title_full_unstemmed A Potential Anticancer Mechanism of Finger Root (Boesenbergia rotunda) Extracts against a Breast Cancer Cell Line
title_short A Potential Anticancer Mechanism of Finger Root (Boesenbergia rotunda) Extracts against a Breast Cancer Cell Line
title_sort potential anticancer mechanism of finger root (boesenbergia rotunda) extracts against a breast cancer cell line
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467824/
https://www.ncbi.nlm.nih.gov/pubmed/36106139
http://dx.doi.org/10.1155/2022/9130252
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