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Carbapenemase- and Colistin Resistant Escherichia coli Strains from Children in China: High Genetic Diversity and First Report of bla(NDM-5), bla(CTX-M-65), bla(OXA-10), bla(TEM-1), and mcr-1.1 Genes Co-Occurrence in E. coli ST156
BACKGROUND: The emergence of carbapenem and colistin co-resistant Escherichia coli poses a huge challenge to infection control. The purpose of this study was to clarify the mechanism of the carbapenems and colistin co-resistance in E. coli strains. METHODS: Antimicrobial susceptibility test was carr...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467903/ https://www.ncbi.nlm.nih.gov/pubmed/36110130 http://dx.doi.org/10.2147/IDR.S378574 |
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author | Zhang, Xiucai Fang, Chao Zhang, Junfeng Hua, Wang He, Rong Zhou, Mingming |
author_facet | Zhang, Xiucai Fang, Chao Zhang, Junfeng Hua, Wang He, Rong Zhou, Mingming |
author_sort | Zhang, Xiucai |
collection | PubMed |
description | BACKGROUND: The emergence of carbapenem and colistin co-resistant Escherichia coli poses a huge challenge to infection control. The purpose of this study was to clarify the mechanism of the carbapenems and colistin co-resistance in E. coli strains. METHODS: Antimicrobial susceptibility test was carried out by agar dilution methods and colistin resistance was confirmed by broth microdilution methods. Whole genome sequencing was carried out, and resistance genes, sequence types and virulence genes of carbapenems and colistin co-resistance E. coli isolates were analyzed. RESULTS: The results showed that among the 176 carbapenem-resistant Enterobacteriaceae strains, 5 multidrug resistant E. coli strains exhibiting coresistance to carbapenem and colistin. The main mechanism of 5 E. coli strains in this study was generating carbapenem. Four E. coli strains were mcr-positive, while one mcr-negative strain had a new MgrB mutation. The blaNDM-5, blaCTX-M-65, blaOXA-10, blaTEM-1 and mcr-1.1 genes were simultaneously detected in E. coli 20IR1127 strain belonging to ST156 lineage. Other antimicrobial resistance genes encoding aminoglycosides-, sulfonamide-, chloramphenicol-, tetracyclines- and macrolides resistance were also detected. CONCLUSION: The main mechanisms of carbapenem and colistin resistance were encoded by bla(NDM) and mcr1.1, meanwhile mgrB mutations also contribute to colistin resistance. To our knowledge, this study is the first to report of E. coli ST156 strain in which the bla(NDM-5), bla(CTX-M-65), bla(OXA-10), bla(TEM-1) and mcr1.1 genes coexist. In addition, there is also an E. coli ST457 strain, which carries bla(TEM-1), bla(NDM-9), bla(CTX-M-199) and is positive for mcr1.1 gene. |
format | Online Article Text |
id | pubmed-9467903 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-94679032022-09-14 Carbapenemase- and Colistin Resistant Escherichia coli Strains from Children in China: High Genetic Diversity and First Report of bla(NDM-5), bla(CTX-M-65), bla(OXA-10), bla(TEM-1), and mcr-1.1 Genes Co-Occurrence in E. coli ST156 Zhang, Xiucai Fang, Chao Zhang, Junfeng Hua, Wang He, Rong Zhou, Mingming Infect Drug Resist Original Research BACKGROUND: The emergence of carbapenem and colistin co-resistant Escherichia coli poses a huge challenge to infection control. The purpose of this study was to clarify the mechanism of the carbapenems and colistin co-resistance in E. coli strains. METHODS: Antimicrobial susceptibility test was carried out by agar dilution methods and colistin resistance was confirmed by broth microdilution methods. Whole genome sequencing was carried out, and resistance genes, sequence types and virulence genes of carbapenems and colistin co-resistance E. coli isolates were analyzed. RESULTS: The results showed that among the 176 carbapenem-resistant Enterobacteriaceae strains, 5 multidrug resistant E. coli strains exhibiting coresistance to carbapenem and colistin. The main mechanism of 5 E. coli strains in this study was generating carbapenem. Four E. coli strains were mcr-positive, while one mcr-negative strain had a new MgrB mutation. The blaNDM-5, blaCTX-M-65, blaOXA-10, blaTEM-1 and mcr-1.1 genes were simultaneously detected in E. coli 20IR1127 strain belonging to ST156 lineage. Other antimicrobial resistance genes encoding aminoglycosides-, sulfonamide-, chloramphenicol-, tetracyclines- and macrolides resistance were also detected. CONCLUSION: The main mechanisms of carbapenem and colistin resistance were encoded by bla(NDM) and mcr1.1, meanwhile mgrB mutations also contribute to colistin resistance. To our knowledge, this study is the first to report of E. coli ST156 strain in which the bla(NDM-5), bla(CTX-M-65), bla(OXA-10), bla(TEM-1) and mcr1.1 genes coexist. In addition, there is also an E. coli ST457 strain, which carries bla(TEM-1), bla(NDM-9), bla(CTX-M-199) and is positive for mcr1.1 gene. Dove 2022-09-08 /pmc/articles/PMC9467903/ /pubmed/36110130 http://dx.doi.org/10.2147/IDR.S378574 Text en © 2022 Zhang et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Zhang, Xiucai Fang, Chao Zhang, Junfeng Hua, Wang He, Rong Zhou, Mingming Carbapenemase- and Colistin Resistant Escherichia coli Strains from Children in China: High Genetic Diversity and First Report of bla(NDM-5), bla(CTX-M-65), bla(OXA-10), bla(TEM-1), and mcr-1.1 Genes Co-Occurrence in E. coli ST156 |
title | Carbapenemase- and Colistin Resistant Escherichia coli Strains from Children in China: High Genetic Diversity and First Report of bla(NDM-5), bla(CTX-M-65), bla(OXA-10), bla(TEM-1), and mcr-1.1 Genes Co-Occurrence in E. coli ST156 |
title_full | Carbapenemase- and Colistin Resistant Escherichia coli Strains from Children in China: High Genetic Diversity and First Report of bla(NDM-5), bla(CTX-M-65), bla(OXA-10), bla(TEM-1), and mcr-1.1 Genes Co-Occurrence in E. coli ST156 |
title_fullStr | Carbapenemase- and Colistin Resistant Escherichia coli Strains from Children in China: High Genetic Diversity and First Report of bla(NDM-5), bla(CTX-M-65), bla(OXA-10), bla(TEM-1), and mcr-1.1 Genes Co-Occurrence in E. coli ST156 |
title_full_unstemmed | Carbapenemase- and Colistin Resistant Escherichia coli Strains from Children in China: High Genetic Diversity and First Report of bla(NDM-5), bla(CTX-M-65), bla(OXA-10), bla(TEM-1), and mcr-1.1 Genes Co-Occurrence in E. coli ST156 |
title_short | Carbapenemase- and Colistin Resistant Escherichia coli Strains from Children in China: High Genetic Diversity and First Report of bla(NDM-5), bla(CTX-M-65), bla(OXA-10), bla(TEM-1), and mcr-1.1 Genes Co-Occurrence in E. coli ST156 |
title_sort | carbapenemase- and colistin resistant escherichia coli strains from children in china: high genetic diversity and first report of bla(ndm-5), bla(ctx-m-65), bla(oxa-10), bla(tem-1), and mcr-1.1 genes co-occurrence in e. coli st156 |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467903/ https://www.ncbi.nlm.nih.gov/pubmed/36110130 http://dx.doi.org/10.2147/IDR.S378574 |
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