DNA-PAINT MINFLUX nanoscopy

MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations, has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on–off switching process for single fluorophore separation, initial MINFLUX implementations...

Descripción completa

Detalles Bibliográficos
Autores principales: Ostersehlt, Lynn M., Jans, Daniel C., Wittek, Anna, Keller-Findeisen, Jan, Inamdar, Kaushik, Sahl, Steffen J., Hell, Stefan W., Jakobs, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467913/
https://www.ncbi.nlm.nih.gov/pubmed/36050490
http://dx.doi.org/10.1038/s41592-022-01577-1
_version_ 1784788296601174016
author Ostersehlt, Lynn M.
Jans, Daniel C.
Wittek, Anna
Keller-Findeisen, Jan
Inamdar, Kaushik
Sahl, Steffen J.
Hell, Stefan W.
Jakobs, Stefan
author_facet Ostersehlt, Lynn M.
Jans, Daniel C.
Wittek, Anna
Keller-Findeisen, Jan
Inamdar, Kaushik
Sahl, Steffen J.
Hell, Stefan W.
Jakobs, Stefan
author_sort Ostersehlt, Lynn M.
collection PubMed
description MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations, has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on–off switching process for single fluorophore separation, initial MINFLUX implementations have been limited to two color channels. Here we show that MINFLUX can be effectively combined with sequentially multiplexed DNA-based labeling (DNA-PAINT), expanding MINFLUX nanoscopy to multiple molecular targets. Our method is exemplified with three-color recordings of mitochondria in human cells.
format Online
Article
Text
id pubmed-9467913
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group US
record_format MEDLINE/PubMed
spelling pubmed-94679132022-09-14 DNA-PAINT MINFLUX nanoscopy Ostersehlt, Lynn M. Jans, Daniel C. Wittek, Anna Keller-Findeisen, Jan Inamdar, Kaushik Sahl, Steffen J. Hell, Stefan W. Jakobs, Stefan Nat Methods Brief Communication MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations, has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on–off switching process for single fluorophore separation, initial MINFLUX implementations have been limited to two color channels. Here we show that MINFLUX can be effectively combined with sequentially multiplexed DNA-based labeling (DNA-PAINT), expanding MINFLUX nanoscopy to multiple molecular targets. Our method is exemplified with three-color recordings of mitochondria in human cells. Nature Publishing Group US 2022-09-01 2022 /pmc/articles/PMC9467913/ /pubmed/36050490 http://dx.doi.org/10.1038/s41592-022-01577-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Brief Communication
Ostersehlt, Lynn M.
Jans, Daniel C.
Wittek, Anna
Keller-Findeisen, Jan
Inamdar, Kaushik
Sahl, Steffen J.
Hell, Stefan W.
Jakobs, Stefan
DNA-PAINT MINFLUX nanoscopy
title DNA-PAINT MINFLUX nanoscopy
title_full DNA-PAINT MINFLUX nanoscopy
title_fullStr DNA-PAINT MINFLUX nanoscopy
title_full_unstemmed DNA-PAINT MINFLUX nanoscopy
title_short DNA-PAINT MINFLUX nanoscopy
title_sort dna-paint minflux nanoscopy
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467913/
https://www.ncbi.nlm.nih.gov/pubmed/36050490
http://dx.doi.org/10.1038/s41592-022-01577-1
work_keys_str_mv AT ostersehltlynnm dnapaintminfluxnanoscopy
AT jansdanielc dnapaintminfluxnanoscopy
AT wittekanna dnapaintminfluxnanoscopy
AT kellerfindeisenjan dnapaintminfluxnanoscopy
AT inamdarkaushik dnapaintminfluxnanoscopy
AT sahlsteffenj dnapaintminfluxnanoscopy
AT hellstefanw dnapaintminfluxnanoscopy
AT jakobsstefan dnapaintminfluxnanoscopy

Ejemplares similares