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Clinical performance of SARS-CoV-2 detection on the cobas Liat using water gargle samples()

Spring water gargle (SWG) is a suitable, non-invasive, alternative specimen for SARS-CoV-2 detection by RT-PCR. This study sought to evaluate the performance of the cobas Liat point-of-care system for the detection of SARS-CoV-2 in SWG samples. SWG samples and standard oral and nasopharyngeal swab (...

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Autores principales: Veillette, Maxime, Tourangeau, Francine, Fafard, Judith, Dumaresq, Jeannot, Labbé, Annie-Claude
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier Ltd. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9468300/
https://www.ncbi.nlm.nih.gov/pubmed/36117531
http://dx.doi.org/10.1016/j.jcvp.2022.100108
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author Veillette, Maxime
Tourangeau, Francine
Fafard, Judith
Dumaresq, Jeannot
Labbé, Annie-Claude
author_facet Veillette, Maxime
Tourangeau, Francine
Fafard, Judith
Dumaresq, Jeannot
Labbé, Annie-Claude
author_sort Veillette, Maxime
collection PubMed
description Spring water gargle (SWG) is a suitable, non-invasive, alternative specimen for SARS-CoV-2 detection by RT-PCR. This study sought to evaluate the performance of the cobas Liat point-of-care system for the detection of SARS-CoV-2 in SWG samples. SWG samples and standard oral and nasopharyngeal swab (ONPS) were collected simultaneously from participants in a COVID-19 screening clinic, in November and December 2020. Both sample types were analyzed in parallel on the cobas Liat platform and with the Seegene Allplex 2019-nCoV assay. Among the 110 participants, 53% had compatible symptoms and 71% had a contact with a confirmed COVID-19 case. Only two (1.8%) individuals had neither symptoms nor contact. Amongst 110 paired samples, 25 (23%) were positive for SARS-CoV-2 on the cobas Liat for a least one sample type, with a kappa coefficient of 0.92. Agreement between the cobas Liat platform and the Seegene assay was also excellent (kappa coefficient values of 0.94 and 0.95). Two SWG samples failed to provide a positive result when their ONPS pair was positive, but their cycle threshold (Ct) values were >35 on the Seegene assay, reflecting a low viral load. Overall, the performance of the cobas Liat platform is excellent for the detection of SARS-CoV-2 in SWG samples in a high pre-test probability population.
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spelling pubmed-94683002022-09-13 Clinical performance of SARS-CoV-2 detection on the cobas Liat using water gargle samples() Veillette, Maxime Tourangeau, Francine Fafard, Judith Dumaresq, Jeannot Labbé, Annie-Claude J Clin Virol Plus Short Communication Spring water gargle (SWG) is a suitable, non-invasive, alternative specimen for SARS-CoV-2 detection by RT-PCR. This study sought to evaluate the performance of the cobas Liat point-of-care system for the detection of SARS-CoV-2 in SWG samples. SWG samples and standard oral and nasopharyngeal swab (ONPS) were collected simultaneously from participants in a COVID-19 screening clinic, in November and December 2020. Both sample types were analyzed in parallel on the cobas Liat platform and with the Seegene Allplex 2019-nCoV assay. Among the 110 participants, 53% had compatible symptoms and 71% had a contact with a confirmed COVID-19 case. Only two (1.8%) individuals had neither symptoms nor contact. Amongst 110 paired samples, 25 (23%) were positive for SARS-CoV-2 on the cobas Liat for a least one sample type, with a kappa coefficient of 0.92. Agreement between the cobas Liat platform and the Seegene assay was also excellent (kappa coefficient values of 0.94 and 0.95). Two SWG samples failed to provide a positive result when their ONPS pair was positive, but their cycle threshold (Ct) values were >35 on the Seegene assay, reflecting a low viral load. Overall, the performance of the cobas Liat platform is excellent for the detection of SARS-CoV-2 in SWG samples in a high pre-test probability population. The Authors. Published by Elsevier Ltd. 2022-11 2022-09-13 /pmc/articles/PMC9468300/ /pubmed/36117531 http://dx.doi.org/10.1016/j.jcvp.2022.100108 Text en © 2022 The Authors. Published by Elsevier Ltd. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
Veillette, Maxime
Tourangeau, Francine
Fafard, Judith
Dumaresq, Jeannot
Labbé, Annie-Claude
Clinical performance of SARS-CoV-2 detection on the cobas Liat using water gargle samples()
title Clinical performance of SARS-CoV-2 detection on the cobas Liat using water gargle samples()
title_full Clinical performance of SARS-CoV-2 detection on the cobas Liat using water gargle samples()
title_fullStr Clinical performance of SARS-CoV-2 detection on the cobas Liat using water gargle samples()
title_full_unstemmed Clinical performance of SARS-CoV-2 detection on the cobas Liat using water gargle samples()
title_short Clinical performance of SARS-CoV-2 detection on the cobas Liat using water gargle samples()
title_sort clinical performance of sars-cov-2 detection on the cobas liat using water gargle samples()
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9468300/
https://www.ncbi.nlm.nih.gov/pubmed/36117531
http://dx.doi.org/10.1016/j.jcvp.2022.100108
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