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Feasibility of membrane ultrafiltration as a single-step clarification and fractionation of microalgal protein hydrolysates

Protein hydrolysates are one of the most valuable products that can be obtained from lipid-extracted microalgae (LEA). The advantages of protein hydrolysates over other protein products encompass enhanced solubility, digestibility, and potential bioactivity. The development of an economically feasib...

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Autores principales: Soto-Sierra, Laura, Nikolov, Zivko L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9468705/
https://www.ncbi.nlm.nih.gov/pubmed/36110323
http://dx.doi.org/10.3389/fbioe.2022.957268
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author Soto-Sierra, Laura
Nikolov, Zivko L.
author_facet Soto-Sierra, Laura
Nikolov, Zivko L.
author_sort Soto-Sierra, Laura
collection PubMed
description Protein hydrolysates are one of the most valuable products that can be obtained from lipid-extracted microalgae (LEA). The advantages of protein hydrolysates over other protein products encompass enhanced solubility, digestibility, and potential bioactivity. The development of an economically feasible process to produce protein hydrolysates depends on maximizing the recovery of hydrolyzed native protein from the lipid-extracted algal biomass and subsequent fractionation of hydrolyzed protein slurry. Previously, we reported a method for fractionation of enzymatically generated protein hydrolysates by acidic precipitation of algal cell debris and unhydrolyzed protein, precipitate wash, centrifugation, and depth filtration. The present study evaluates tangential flow ultrafiltration as a single-step alternative to centrifugation, precipitate wash, and depth filtration. The results demonstrate that the tangential flow ultrafiltration process has a potential that deserves further investigation. First, the membrane diafiltration process uses a single and easily scalable unit operation (tangential flow filtration) to separate and “wash out” hydrolyzed protein from the algal residue. Second, the protein recovery yield achieved with the tangential flow process was >70% compared to 64% previously achieved by centrifugation and depth filtration methods. Finally, protein hydrolysates obtained by membrane ultrafiltration exhibited slightly better heat and pH stability.
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spelling pubmed-94687052022-09-14 Feasibility of membrane ultrafiltration as a single-step clarification and fractionation of microalgal protein hydrolysates Soto-Sierra, Laura Nikolov, Zivko L. Front Bioeng Biotechnol Bioengineering and Biotechnology Protein hydrolysates are one of the most valuable products that can be obtained from lipid-extracted microalgae (LEA). The advantages of protein hydrolysates over other protein products encompass enhanced solubility, digestibility, and potential bioactivity. The development of an economically feasible process to produce protein hydrolysates depends on maximizing the recovery of hydrolyzed native protein from the lipid-extracted algal biomass and subsequent fractionation of hydrolyzed protein slurry. Previously, we reported a method for fractionation of enzymatically generated protein hydrolysates by acidic precipitation of algal cell debris and unhydrolyzed protein, precipitate wash, centrifugation, and depth filtration. The present study evaluates tangential flow ultrafiltration as a single-step alternative to centrifugation, precipitate wash, and depth filtration. The results demonstrate that the tangential flow ultrafiltration process has a potential that deserves further investigation. First, the membrane diafiltration process uses a single and easily scalable unit operation (tangential flow filtration) to separate and “wash out” hydrolyzed protein from the algal residue. Second, the protein recovery yield achieved with the tangential flow process was >70% compared to 64% previously achieved by centrifugation and depth filtration methods. Finally, protein hydrolysates obtained by membrane ultrafiltration exhibited slightly better heat and pH stability. Frontiers Media S.A. 2022-08-30 /pmc/articles/PMC9468705/ /pubmed/36110323 http://dx.doi.org/10.3389/fbioe.2022.957268 Text en Copyright © 2022 Soto-Sierra and Nikolov. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Soto-Sierra, Laura
Nikolov, Zivko L.
Feasibility of membrane ultrafiltration as a single-step clarification and fractionation of microalgal protein hydrolysates
title Feasibility of membrane ultrafiltration as a single-step clarification and fractionation of microalgal protein hydrolysates
title_full Feasibility of membrane ultrafiltration as a single-step clarification and fractionation of microalgal protein hydrolysates
title_fullStr Feasibility of membrane ultrafiltration as a single-step clarification and fractionation of microalgal protein hydrolysates
title_full_unstemmed Feasibility of membrane ultrafiltration as a single-step clarification and fractionation of microalgal protein hydrolysates
title_short Feasibility of membrane ultrafiltration as a single-step clarification and fractionation of microalgal protein hydrolysates
title_sort feasibility of membrane ultrafiltration as a single-step clarification and fractionation of microalgal protein hydrolysates
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9468705/
https://www.ncbi.nlm.nih.gov/pubmed/36110323
http://dx.doi.org/10.3389/fbioe.2022.957268
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