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Exploring the mechanisms of neurotoxicity caused by fuzi using network pharmacology and molecular docking
Safety has always been an important issue affecting the development of traditional Chinese medicine industry, especially for toxic medicinal materials, the establishment of risk prevention and control measures for toxic herbs is of great significance to improving the use of traditional Chinese medic...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9468872/ https://www.ncbi.nlm.nih.gov/pubmed/36110545 http://dx.doi.org/10.3389/fphar.2022.961012 |
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author | An, Junsha Fan, Huali Han, Mingyu Peng, Cheng Xie, Jie Peng, Fu |
author_facet | An, Junsha Fan, Huali Han, Mingyu Peng, Cheng Xie, Jie Peng, Fu |
author_sort | An, Junsha |
collection | PubMed |
description | Safety has always been an important issue affecting the development of traditional Chinese medicine industry, especially for toxic medicinal materials, the establishment of risk prevention and control measures for toxic herbs is of great significance to improving the use of traditional Chinese medicine in clinical. Fuzi is a kind of traditional Chinese medicine and its toxicity has become the most important obstacle of limit in clinical using. In this paper, network pharmacology and molecular docking technology were used to analyze the main toxic components of Fuzi, the key targets and the mechanism of neurotoxicity. We carried out CCK-8 and WB assays, and detected LDH release and SDH activity. It was verified that aconitine caused neurotoxicity through a variety of pathways, including MAPK signaling pathway, pathways related to Akt protein, destruction of cell membrane integrity, damage of mitochondrial function affecting energy metabolism and apoptosis. What’s more, this study confirmed that aconitine could produce neurotoxicity by promoting apoptosis of hippocampus neuron and decreasing its quantity through Nissl Staining and TUNEL assay. This paper found and confirmed multiple targets and various pathways causing neurotoxicity of Fuzi, in order to provide reference for clinical application and related research. |
format | Online Article Text |
id | pubmed-9468872 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94688722022-09-14 Exploring the mechanisms of neurotoxicity caused by fuzi using network pharmacology and molecular docking An, Junsha Fan, Huali Han, Mingyu Peng, Cheng Xie, Jie Peng, Fu Front Pharmacol Pharmacology Safety has always been an important issue affecting the development of traditional Chinese medicine industry, especially for toxic medicinal materials, the establishment of risk prevention and control measures for toxic herbs is of great significance to improving the use of traditional Chinese medicine in clinical. Fuzi is a kind of traditional Chinese medicine and its toxicity has become the most important obstacle of limit in clinical using. In this paper, network pharmacology and molecular docking technology were used to analyze the main toxic components of Fuzi, the key targets and the mechanism of neurotoxicity. We carried out CCK-8 and WB assays, and detected LDH release and SDH activity. It was verified that aconitine caused neurotoxicity through a variety of pathways, including MAPK signaling pathway, pathways related to Akt protein, destruction of cell membrane integrity, damage of mitochondrial function affecting energy metabolism and apoptosis. What’s more, this study confirmed that aconitine could produce neurotoxicity by promoting apoptosis of hippocampus neuron and decreasing its quantity through Nissl Staining and TUNEL assay. This paper found and confirmed multiple targets and various pathways causing neurotoxicity of Fuzi, in order to provide reference for clinical application and related research. Frontiers Media S.A. 2022-08-30 /pmc/articles/PMC9468872/ /pubmed/36110545 http://dx.doi.org/10.3389/fphar.2022.961012 Text en Copyright © 2022 An, Fan, Han, Peng, Xie and Peng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology An, Junsha Fan, Huali Han, Mingyu Peng, Cheng Xie, Jie Peng, Fu Exploring the mechanisms of neurotoxicity caused by fuzi using network pharmacology and molecular docking |
title | Exploring the mechanisms of neurotoxicity caused by fuzi using network pharmacology and molecular docking |
title_full | Exploring the mechanisms of neurotoxicity caused by fuzi using network pharmacology and molecular docking |
title_fullStr | Exploring the mechanisms of neurotoxicity caused by fuzi using network pharmacology and molecular docking |
title_full_unstemmed | Exploring the mechanisms of neurotoxicity caused by fuzi using network pharmacology and molecular docking |
title_short | Exploring the mechanisms of neurotoxicity caused by fuzi using network pharmacology and molecular docking |
title_sort | exploring the mechanisms of neurotoxicity caused by fuzi using network pharmacology and molecular docking |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9468872/ https://www.ncbi.nlm.nih.gov/pubmed/36110545 http://dx.doi.org/10.3389/fphar.2022.961012 |
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