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An In Vitro Comparison of the Effect of Wound Irrigating Solution (0.01% Hypochlorous Acid) and 2.5% Sodium Hypochlorite Against Enterococcus Faecalis

AIM: The aim of this in vitro study was to evaluate and compare the antimicrobial activity of normal saline, 2.5% sodium hypochlorite, and 0.01% hypochlorous acid against Enterococcus faecalis. MATERIALS AND METHODS: Antimicrobial activity was assayed by Kirby–Bauer cup-well agar diffusion method. T...

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Autores principales: Anwar, Saif, Sivalingam, Balaji, Vijayakumar, Nyklesh, Vivek, K, Perumal, Lavanya P. K., Prabha, Esai A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9469257/
https://www.ncbi.nlm.nih.gov/pubmed/36110823
http://dx.doi.org/10.4103/jpbs.jpbs_172_22
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author Anwar, Saif
Sivalingam, Balaji
Vijayakumar, Nyklesh
Vivek, K
Perumal, Lavanya P. K.
Prabha, Esai A.
author_facet Anwar, Saif
Sivalingam, Balaji
Vijayakumar, Nyklesh
Vivek, K
Perumal, Lavanya P. K.
Prabha, Esai A.
author_sort Anwar, Saif
collection PubMed
description AIM: The aim of this in vitro study was to evaluate and compare the antimicrobial activity of normal saline, 2.5% sodium hypochlorite, and 0.01% hypochlorous acid against Enterococcus faecalis. MATERIALS AND METHODS: Antimicrobial activity was assayed by Kirby–Bauer cup-well agar diffusion method. Three cup-wells were bored in each petriplate and were filled with 50 μl of each of the respective test agents. Culture plates were placed in an incubator at 37°C and after 24 hours, the size of the magnification inhibition zone was quantified with a caliper and recorded in respective tables. The experiment was reiterated ten times for each reagent, and the diameter of the magnification inhibition zones caused by the irrigation solutions was recorded. The antimicrobial activity of the three test solutions was determined. RESULTS: The antibacterial property of the three groups was compared by utilizing ANOVA test. The association was found to be statistically significant (P = 0.000). The antibacterial property between 2.5% NaOCl and 0.01% HOCl was further compared with independent t test. This association was however not statistically significant (P = 0.02).
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spelling pubmed-94692572022-09-14 An In Vitro Comparison of the Effect of Wound Irrigating Solution (0.01% Hypochlorous Acid) and 2.5% Sodium Hypochlorite Against Enterococcus Faecalis Anwar, Saif Sivalingam, Balaji Vijayakumar, Nyklesh Vivek, K Perumal, Lavanya P. K. Prabha, Esai A. J Pharm Bioallied Sci Original Article AIM: The aim of this in vitro study was to evaluate and compare the antimicrobial activity of normal saline, 2.5% sodium hypochlorite, and 0.01% hypochlorous acid against Enterococcus faecalis. MATERIALS AND METHODS: Antimicrobial activity was assayed by Kirby–Bauer cup-well agar diffusion method. Three cup-wells were bored in each petriplate and were filled with 50 μl of each of the respective test agents. Culture plates were placed in an incubator at 37°C and after 24 hours, the size of the magnification inhibition zone was quantified with a caliper and recorded in respective tables. The experiment was reiterated ten times for each reagent, and the diameter of the magnification inhibition zones caused by the irrigation solutions was recorded. The antimicrobial activity of the three test solutions was determined. RESULTS: The antibacterial property of the three groups was compared by utilizing ANOVA test. The association was found to be statistically significant (P = 0.000). The antibacterial property between 2.5% NaOCl and 0.01% HOCl was further compared with independent t test. This association was however not statistically significant (P = 0.02). Wolters Kluwer - Medknow 2022-07 2022-07-13 /pmc/articles/PMC9469257/ /pubmed/36110823 http://dx.doi.org/10.4103/jpbs.jpbs_172_22 Text en Copyright: © 2022 Journal of Pharmacy and Bioallied Sciences https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Anwar, Saif
Sivalingam, Balaji
Vijayakumar, Nyklesh
Vivek, K
Perumal, Lavanya P. K.
Prabha, Esai A.
An In Vitro Comparison of the Effect of Wound Irrigating Solution (0.01% Hypochlorous Acid) and 2.5% Sodium Hypochlorite Against Enterococcus Faecalis
title An In Vitro Comparison of the Effect of Wound Irrigating Solution (0.01% Hypochlorous Acid) and 2.5% Sodium Hypochlorite Against Enterococcus Faecalis
title_full An In Vitro Comparison of the Effect of Wound Irrigating Solution (0.01% Hypochlorous Acid) and 2.5% Sodium Hypochlorite Against Enterococcus Faecalis
title_fullStr An In Vitro Comparison of the Effect of Wound Irrigating Solution (0.01% Hypochlorous Acid) and 2.5% Sodium Hypochlorite Against Enterococcus Faecalis
title_full_unstemmed An In Vitro Comparison of the Effect of Wound Irrigating Solution (0.01% Hypochlorous Acid) and 2.5% Sodium Hypochlorite Against Enterococcus Faecalis
title_short An In Vitro Comparison of the Effect of Wound Irrigating Solution (0.01% Hypochlorous Acid) and 2.5% Sodium Hypochlorite Against Enterococcus Faecalis
title_sort in vitro comparison of the effect of wound irrigating solution (0.01% hypochlorous acid) and 2.5% sodium hypochlorite against enterococcus faecalis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9469257/
https://www.ncbi.nlm.nih.gov/pubmed/36110823
http://dx.doi.org/10.4103/jpbs.jpbs_172_22
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