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Overexpression of cotton GhNAC072 gene enhances drought and salt stress tolerance in transgenic Arabidopsis

BACKGROUND: Crops face several environmental stresses (biotic and abiotic), thus resulting in severe yield losses. Around the globe abiotic stresses are the main contributors of plant damages, primarily drought and salinity. Many genes and transcription factors are involved in abiotic and biotic str...

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Autores principales: Mehari, Teame Gereziher, Hou, Yuqing, Xu, Yanchao, Umer, Muhammad Jawad, Shiraku, Margaret Linyerera, Wang, Yuhong, Wang, Heng, Peng, Renhai, Wei, Yangyang, Cai, Xiaoyan, Zhou, Zhongli, Liu, Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9469605/
https://www.ncbi.nlm.nih.gov/pubmed/36096725
http://dx.doi.org/10.1186/s12864-022-08876-z
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author Mehari, Teame Gereziher
Hou, Yuqing
Xu, Yanchao
Umer, Muhammad Jawad
Shiraku, Margaret Linyerera
Wang, Yuhong
Wang, Heng
Peng, Renhai
Wei, Yangyang
Cai, Xiaoyan
Zhou, Zhongli
Liu, Fang
author_facet Mehari, Teame Gereziher
Hou, Yuqing
Xu, Yanchao
Umer, Muhammad Jawad
Shiraku, Margaret Linyerera
Wang, Yuhong
Wang, Heng
Peng, Renhai
Wei, Yangyang
Cai, Xiaoyan
Zhou, Zhongli
Liu, Fang
author_sort Mehari, Teame Gereziher
collection PubMed
description BACKGROUND: Crops face several environmental stresses (biotic and abiotic), thus resulting in severe yield losses. Around the globe abiotic stresses are the main contributors of plant damages, primarily drought and salinity. Many genes and transcription factors are involved in abiotic and biotic stress responses. NAC TF (Transcription Factors) improves tolerance to stresses by controlling the physiological and enzyme activities of crops. RESULTS: In current research, GhNAC072 a highly upregulated TF in RNA-Seq was identified as a hub gene in the co-expression network analysis (WGCNA). This gene was transformed to Arabidopsis thaliana to confirm its potential role in drought and salt stress tolerance. Significant variations were observed in the morpho-physiological traits with high relative leaf water contents, chlorophyll contents, higher germination and longer root lengths of the overexpressed lines and low excised leaf loss and ion leakage as compared to the wildtype plants. Besides, overexpressed lines have higher amounts of antioxidants and low oxidant enzyme activities than the wildtype during the period of stress exposure. CONCLUSIONS: In summary, the above analysis showed that GhNAC072 might be the true candidate involved in boosting tolerance mechanisms under drought and salinity stress. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08876-z.
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spelling pubmed-94696052022-09-14 Overexpression of cotton GhNAC072 gene enhances drought and salt stress tolerance in transgenic Arabidopsis Mehari, Teame Gereziher Hou, Yuqing Xu, Yanchao Umer, Muhammad Jawad Shiraku, Margaret Linyerera Wang, Yuhong Wang, Heng Peng, Renhai Wei, Yangyang Cai, Xiaoyan Zhou, Zhongli Liu, Fang BMC Genomics Research BACKGROUND: Crops face several environmental stresses (biotic and abiotic), thus resulting in severe yield losses. Around the globe abiotic stresses are the main contributors of plant damages, primarily drought and salinity. Many genes and transcription factors are involved in abiotic and biotic stress responses. NAC TF (Transcription Factors) improves tolerance to stresses by controlling the physiological and enzyme activities of crops. RESULTS: In current research, GhNAC072 a highly upregulated TF in RNA-Seq was identified as a hub gene in the co-expression network analysis (WGCNA). This gene was transformed to Arabidopsis thaliana to confirm its potential role in drought and salt stress tolerance. Significant variations were observed in the morpho-physiological traits with high relative leaf water contents, chlorophyll contents, higher germination and longer root lengths of the overexpressed lines and low excised leaf loss and ion leakage as compared to the wildtype plants. Besides, overexpressed lines have higher amounts of antioxidants and low oxidant enzyme activities than the wildtype during the period of stress exposure. CONCLUSIONS: In summary, the above analysis showed that GhNAC072 might be the true candidate involved in boosting tolerance mechanisms under drought and salinity stress. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08876-z. BioMed Central 2022-09-12 /pmc/articles/PMC9469605/ /pubmed/36096725 http://dx.doi.org/10.1186/s12864-022-08876-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Mehari, Teame Gereziher
Hou, Yuqing
Xu, Yanchao
Umer, Muhammad Jawad
Shiraku, Margaret Linyerera
Wang, Yuhong
Wang, Heng
Peng, Renhai
Wei, Yangyang
Cai, Xiaoyan
Zhou, Zhongli
Liu, Fang
Overexpression of cotton GhNAC072 gene enhances drought and salt stress tolerance in transgenic Arabidopsis
title Overexpression of cotton GhNAC072 gene enhances drought and salt stress tolerance in transgenic Arabidopsis
title_full Overexpression of cotton GhNAC072 gene enhances drought and salt stress tolerance in transgenic Arabidopsis
title_fullStr Overexpression of cotton GhNAC072 gene enhances drought and salt stress tolerance in transgenic Arabidopsis
title_full_unstemmed Overexpression of cotton GhNAC072 gene enhances drought and salt stress tolerance in transgenic Arabidopsis
title_short Overexpression of cotton GhNAC072 gene enhances drought and salt stress tolerance in transgenic Arabidopsis
title_sort overexpression of cotton ghnac072 gene enhances drought and salt stress tolerance in transgenic arabidopsis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9469605/
https://www.ncbi.nlm.nih.gov/pubmed/36096725
http://dx.doi.org/10.1186/s12864-022-08876-z
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