Generation of self-assembling cardiac organoids using hiPSC-derived cardiomyocytes

The combination of the human induced pluripotent stem cell (hiPSC) and organoid technology enables the generation of human 3D culture systems, providing the opportunity to model human tissue-like structures in vitro. This protocol offers the details to generate and characterize self-assembling 3D ca...

Descripción completa

Detalles Bibliográficos
Autores principales: Künzel, Karolina, Künzel, Stephan R., Guan, Kaomei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9469666/
https://www.ncbi.nlm.nih.gov/pubmed/36110234
http://dx.doi.org/10.1016/j.heliyon.2022.e10365
_version_ 1784788691196051456
author Künzel, Karolina
Künzel, Stephan R.
Guan, Kaomei
author_facet Künzel, Karolina
Künzel, Stephan R.
Guan, Kaomei
author_sort Künzel, Karolina
collection PubMed
description The combination of the human induced pluripotent stem cell (hiPSC) and organoid technology enables the generation of human 3D culture systems, providing the opportunity to model human tissue-like structures in vitro. This protocol offers the details to generate and characterize self-assembling 3D cardiac organoids in a controlled and efficient manner based on hiPSC-derived cardiomyocytes. Cardiac organoids can be used as 3D-based assay systems and offer a wide range of applications in pharmacological and toxicological research as well as an alternative to animal experiments.
format Online
Article
Text
id pubmed-9469666
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-94696662022-09-14 Generation of self-assembling cardiac organoids using hiPSC-derived cardiomyocytes Künzel, Karolina Künzel, Stephan R. Guan, Kaomei Heliyon Research Article The combination of the human induced pluripotent stem cell (hiPSC) and organoid technology enables the generation of human 3D culture systems, providing the opportunity to model human tissue-like structures in vitro. This protocol offers the details to generate and characterize self-assembling 3D cardiac organoids in a controlled and efficient manner based on hiPSC-derived cardiomyocytes. Cardiac organoids can be used as 3D-based assay systems and offer a wide range of applications in pharmacological and toxicological research as well as an alternative to animal experiments. Elsevier 2022-08-28 /pmc/articles/PMC9469666/ /pubmed/36110234 http://dx.doi.org/10.1016/j.heliyon.2022.e10365 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Künzel, Karolina
Künzel, Stephan R.
Guan, Kaomei
Generation of self-assembling cardiac organoids using hiPSC-derived cardiomyocytes
title Generation of self-assembling cardiac organoids using hiPSC-derived cardiomyocytes
title_full Generation of self-assembling cardiac organoids using hiPSC-derived cardiomyocytes
title_fullStr Generation of self-assembling cardiac organoids using hiPSC-derived cardiomyocytes
title_full_unstemmed Generation of self-assembling cardiac organoids using hiPSC-derived cardiomyocytes
title_short Generation of self-assembling cardiac organoids using hiPSC-derived cardiomyocytes
title_sort generation of self-assembling cardiac organoids using hipsc-derived cardiomyocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9469666/
https://www.ncbi.nlm.nih.gov/pubmed/36110234
http://dx.doi.org/10.1016/j.heliyon.2022.e10365
work_keys_str_mv AT kunzelkarolina generationofselfassemblingcardiacorganoidsusinghipscderivedcardiomyocytes
AT kunzelstephanr generationofselfassemblingcardiacorganoidsusinghipscderivedcardiomyocytes
AT guankaomei generationofselfassemblingcardiacorganoidsusinghipscderivedcardiomyocytes

Ejemplares similares