Electrochemical Capillary Driven Immunoassay for Detection of SARS-CoV-2

[Image: see text] The COVID-19 pandemic focused attention on a pressing need for fast, accurate, and low-cost diagnostic tests. This work presents an electrochemical capillary driven immunoassay (eCaDI) developed to detect SARS-CoV-2 nucleocapsid (N) protein. The low-cost flow device is made of poly...

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Detalles Bibliográficos
Autores principales: Clark, Kaylee M., Schenkel, Melissa S., Pittman, Trey W., Samper, Isabelle C., Anderson, Loran B. R., Khamcharoen, Wisarut, Elmegerhi, Suad, Perera, Rushika, Siangproh, Weena, Kennan, Alan J., Geiss, Brian J., Dandy, David S., Henry, Charles S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9469961/
https://www.ncbi.nlm.nih.gov/pubmed/36570470
http://dx.doi.org/10.1021/acsmeasuresciau.2c00037
Descripción
Sumario:[Image: see text] The COVID-19 pandemic focused attention on a pressing need for fast, accurate, and low-cost diagnostic tests. This work presents an electrochemical capillary driven immunoassay (eCaDI) developed to detect SARS-CoV-2 nucleocapsid (N) protein. The low-cost flow device is made of polyethylene terephthalate (PET) and adhesive films. Upon addition of a sample, reagents and washes are sequentially delivered to an integrated screen-printed carbon electrode for detection, thus automating a full sandwich immunoassay with a single end-user step. The modified electrodes are sensitive and selective for SARS-CoV-2 N protein and stable for over 7 weeks. The eCaDI was tested with influenza A and Sindbis virus and proved to be selective. The eCaDI was also successfully applied to detect nine different SARS-CoV-2 variants, including Omicron.

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